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Plasmid#42242DepositorInsertgRNA-drd3 (drd3 Zebrafish)
UseCRISPR; Zebrafish expressionTagsExpressionMutationPromoterT7Available sinceJan. 31, 2013AvailabilityAcademic Institutions and Nonprofits only -
p415 δCLD2α
Plasmid#73874PurposeδCLD2α - His tagged (μHD deleted)DepositorInsertArcn1/ δCLD2α (ARCN1 Bovine)
UseTagsHisExpressionYeastMutationPromoterMet25Available sinceDec. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3 T7 Jip1(1-470)
Plasmid#51724Purpose" JIP-1 may suppress signal transduction by the JNK pathway"DepositorInsertJnk interacting protein 1 (Mapk8ip1 Mouse)
UseTagsT7ExpressionMammalianMutationdeleted 471-660PromoterAvailable sinceMarch 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3 T7 Jip1(471-660)
Plasmid#51766Purpose" JIP-1 may suppress signal transduction by the JNK pathway"DepositorInsertJnk interacting protein 1 (Mapk8ip1 Mouse)
UseTagsT7ExpressionMammalianMutationdeleted 1-470PromoterAvailable sinceMarch 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcdna3 T7Jip1(1-126)
Plasmid#51702Purpose" JIP-1 may suppress signal transduction by the JNK pathway"DepositorInsertJnk interacting protein 1 (Mapk8ip1 Mouse)
UseTagsT7ExpressionMammalianMutationdeleted 127-660PromoterAvailable sinceMarch 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3 T7 Jip1(1-281)
Plasmid#51700Purpose" JIP-1 may suppress signal transduction by the JNK pathway"DepositorInsertJnk interacting protein 1 (Mapk8ip1 Mouse)
UseTagsT7ExpressionMammalianMutationdeleted 282-660PromoterAvailable sinceMarch 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
pNS20-SpCas9-SNAP
Plasmid#113717PurposeBacterial vector for expression of Snap-tagged Streptococcus pyogenes Cas9DepositorInsertCas9
UseCRISPRTagsHA-2xNLS-SNAP-NLS and His6-MBP-TEVExpressionBacterialMutationPromoterAvailable sinceSept. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pNS38-SadCas9-SNAP
Plasmid#113718PurposeBacterial vector for expression of Snap-tagged Staphylococcus aureus dCas9DepositorInsertCas9
UseCRISPRTagsHA-2xNLS-SNAP-NLS and His6-MBP-TEVExpressionBacterialMutationD10A, N580APromoterAvailable sinceSept. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLKO5d.SFFV.eGFP-miR30n
Plasmid#90333PurposemiR30 based shMir knockdownDepositorInsertseGFP
na
SFFV
miR30n
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMarch 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
CD 44 pGL4basic
Plasmid#78135Purposeluciferase reporter for miRNA activityDepositorInsertCD 44 (CD44 Human)
UseLuciferaseTagsluciferaseExpressionMammalianMutationPromoterCD44Available sinceMay 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pNS19-LV-mutRFP-2A-GFP
Plasmid#113716PurposeLentiviral vector for fluorescent reporter system: expresses a green fluorescent protein that is preceded by an inactive version of a red fluorescent protein (2 ntd substitution at the fluorophore)DepositorInsertmutRFP-2A-GFP-IRES-Puro
UseLentiviral; Reporter systemTagsExpressionMammalianMutationY64L (mutRFP)PromoterAvailable sinceAug. 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLKO5d.SFFV.dTomato-miR30n
Plasmid#90334PurposemiR30 based shMir knockdownDepositorInsertsdTomato
na
SFFV
miR30n
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMarch 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
Zebrafish-gRNA-0004
Plasmid#42244DepositorInsertgRNA-fh site #2 (fh Zebrafish)
UseCRISPR; Zebrafish expressionTagsExpressionMutationPromoterT7Available sinceJan. 31, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3 T7 Jip1(delta JBD)
Plasmid#51767Purpose" JIP-1 may suppress signal transduction by the JNK pathway"DepositorInsertJnk interacting protein 1 (Mapk8ip1 Mouse)
UseTagsT7ExpressionMammalianMutationdeleted 126-263PromoterAvailable sinceMarch 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
373_ESR1 in pmiRGlo
Plasmid#78134Purposeluciferase reporter for miRNA activityDepositorInsertESR1 (ESR1 Human)
UseLuciferaseTagsluciferaseExpressionMammalianMutation3'UTR containing miRNA binding sitesPromoterPGKAvailable sinceMay 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
Zebrafish-gRNA-0003
Plasmid#42243DepositorInsertgRNA-fh site #1 (fh Zebrafish)
UseCRISPR; Zebrafish expressionTagsExpressionMutationPromoterT7Available sinceJan. 31, 2013AvailabilityAcademic Institutions and Nonprofits only -
pDONOR-PARK6e5-I368N
Plasmid#86154PurposeDonor plasmid for PARK6 exon5 I368N sequence. Also contains TagBFP and dTomatoDepositorInsertPINK1 exon 5 homology arms (PINK1 Human)
UseTagsExpressionBacterial and MammalianMutationPromoterAvailable sinceJune 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcdna3 T7Jip1(282-470)
Plasmid#51703Purpose" JIP-1 may suppress signal transduction by the JNK pathway"DepositorInsertJnk interacting protein 1 (Mapk8ip1 Mouse)
UseTagsT7ExpressionMammalianMutationdeleted 1-281 and 471-660PromoterAvailable sinceMarch 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLKO5d.SFFV.dCas9-KRAB.P2A.BSD
Plasmid#90332PurposeCRISPR interferenceDepositorInsertsdCas9
na
SFFV
P2A-BSD
UseLentiviralTagsHAExpressionMutationPromoterAvailable sinceMarch 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pETM6-G6-vioABE
Plasmid#73438PurposeProdeoxyviolacein pathway (vioABE) in monocistronic configuration, transcriptionally driven by orthogonal T7-lac promoter variant G6.DepositorInsertPG6-vioABE
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterPG6 (orthogonal T7-lac variant)Available sinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits only