Addgene

We narrowed to 5,447 results for: arl

Showing: 4961 - 4980 of 5447 results

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pJW1947

Plasmid

#154339

Purpose

Enhancer reporter

Depositor

Jordan Ward

Article

Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

Insert

pes-10delta::SV40 NLS::mScarlet_I(dpi)::-tbb-2 3'UTR

Use

Tags

Expression

Worm

Mutation

Silent mutations to remove piRNA sites

Promoter

Available since

Aug. 19, 2020

Availability

Academic Institutions and Nonprofits only

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G-GECO1.2-Orai1Y80E

Plasmid

#73565

Purpose

Green fluorescent reporter of mutant Orai1Y80E-associated calcium influx

Depositor

Michael Cahalan

Article

Dynes et al Proc Natl Acad Sci U S A. 2016 Jan 12;11

Insert

G-GECO1.2-Orai1Y80E (ORAI1 Synthetic, Human)

Use

Tags

G-GECO1.2

Expression

Mammalian

Mutation

changed tyrosine 80 of Orai1 to glutamate

Promoter

CMV Immediate Early

Available since

March 11, 2016

Availability

Academic Institutions and Nonprofits only

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pRVdGL-1Cre

Plasmid

#98038

Purpose

Second-generation rabies viral vector genome

Depositor

Ian Wickersham

Article

Chatterjee et al Nat Neurosci. 2018 Apr;21(4):638-646. do

Insert

mCre

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 6, 2018

Availability

Academic Institutions and Nonprofits only

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pRVdGL-4EGFP

Plasmid

#98037

Purpose

Second-generation rabies viral vector genome

Depositor

Ian Wickersham

Article

Chatterjee et al Nat Neurosci. 2018 Apr;21(4):638-646. do

Insert

EGFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 6, 2018

Availability

Academic Institutions and Nonprofits only

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pRVdG-4GCaMP6f

Plasmid

#98035

Purpose

First-generation rabies viral vector genome

Depositor

Ian Wickersham

Article

Chatterjee et al Nat Neurosci. 2018 Apr;21(4):638-646. do

Insert

GCaMP6f

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 6, 2018

Availability

Academic Institutions and Nonprofits only

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pAG247

Plasmid

#33200

Depositor

David Bartel

Article

Garcia et al Nat Struct Mol Biol. 2011 Sep 11;18(10):

Insert

LRIG1 (LRIG1 Human)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Mutation

Promoter

SV40 early enhancer/promoter

Available since

Jan. 10, 2012

Availability

Academic Institutions and Nonprofits only
DF-UAS-sspa-GCaMP6f

Plasmid

#67569

Purpose

Zebrafish expression of a short photoactivatable fluorescent calcium reporter

Depositor

Ehud Isacoff

Article

Berlin et al Nat Methods. 2015 Jul 13. doi: 10.1038/n

Insert

sspa-GCaMP6f

Use

Zebrafish transgenesis (see :a spinal opsin contr…

Tags

Expression

Mutation

K65T, I83V, G87S, Y92D, V115H, K118V, S187R, Y196…

Promoter

Available since

Aug. 13, 2015

Availability

Academic Institutions and Nonprofits only
pLV_mU6-sgNGN3_hU6-sgRNA_hUbC-PuroR-P2A-GFP

Plasmid

#162336

Purpose

Lentiviral expression of sgNGN3 paired with a second S. pyogenes sgRNA with a GFP-P2A-PuroR selection marker

Depositor

Charles Gersbach

Article

Black et al Cell Rep. 2020 Dec 1;33(9):108460. doi:

Insert

S. pyogenes sgRNA

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6; mU6

Available since

Feb. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pFLAG-CMV2 MAML LHHLL

Plasmid

#87681

Purpose

Mammalian expression vector for Maml1 with LHHLL mutation

Depositor

Karl Munger

Article

Meyers et al PLoS Pathog. 2017 Jan 20;13(1):e1006171.

Insert

MAML1 (MAML1 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Mutate 1010,1011 from DD to HH

Promoter

CMV

Available since

March 30, 2017

Availability

Academic Institutions and Nonprofits only

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pSG5 EDD C2768A

Plasmid

#37192

Depositor

Darren Saunders, Charles Watts

Article

Henderson et al J Biol Chem. 2002 Jul 19;277(29):26468-7

Insert

EDD C2768A (UBR5 Human)

Use

Tags

6x His Tag and FLAG

Expression

Mammalian

Mutation

Changed Cysteine 2768 to Alanine; K503R (please s…

Promoter

SV40

Available since

Aug. 2, 2012

Availability

Academic Institutions and Nonprofits only

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pCMV dCas9-MC (eGFP)

Plasmid

#89931

Purpose

Expresses the dCas9-MC fragment only of the sMTase system for targeted DNA methylation in mammalian cells. Contains a eGFP marker expressed off separate promoter.

Depositor

Carl Novina

Article

Xiong et al Sci Rep. 2017 Jul 27;7(1):6732. doi: 10.

Insert

dCas9-MC

Use

Tags

Flag

Expression

Mammalian

Mutation

deactivated Cas9, fragment of M.SssI (residues 27…

Promoter

CMV

Available since

Sept. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pRVdGL-1EGFP

Plasmid

#98036

Purpose

Second-generation rabies viral vector genome

Depositor

Ian Wickersham

Article

Chatterjee et al Nat Neurosci. 2018 Apr;21(4):638-646. do

Insert

EGFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 6, 2018

Availability

Academic Institutions and Nonprofits only

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pENTR CMVie-Lifeact-iRFP670 L1-R5

Plasmid

#84390

Purpose

Multisite Gateway entry vector for expression of iRFP670-tagged Lifeact in cells including neurons

Depositor

Ghassan Mouneimne

Article

Padilla-Rodriguez et al Nat Commun. 2018 Jul 30;9(1):2980. doi:

Insert

Lifeact

Use

Gateway

Tags

iRFP670

Expression

Mammalian

Mutation

Promoter

CMV immediate early

Available since

Jan. 5, 2017

Availability

Academic Institutions and Nonprofits only

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pRVdG-4Cre

Plasmid

#98034

Purpose

First-generation rabies viral vector genome

Depositor

Ian Wickersham

Article

Chatterjee et al Nat Neurosci. 2018 Apr;21(4):638-646. do

Insert

mCre

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 6, 2018

Availability

Academic Institutions and Nonprofits only

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actc1b-mKate2-EEA1

Plasmid

#109508

Purpose

Muscle specific zebrafish expression of mKate2 fused to early endosome associated 1

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

EEA1 (eea1 Zebrafish)

Use

Zebrafish plasmids

Tags

mKate2

Expression

Mutation

Promoter

Available since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pJW1886

Plasmid

#154333

Purpose

Multi-cassette for SapTrap

Depositor

Jordan Ward

Article

Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

Insert

30aa linker::mScarlet-I^SEC (Lox511I)^TEV::degron::3xFLAG

Use

CRISPR and Cre/Lox

Tags

Expression

Worm

Mutation

Promoter

Available since

Aug. 19, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA-Flag-EcoCas6

Plasmid

#106274

Purpose

Encodes E.coli CRISPR Type I-E CasE with N-terminal fusion of Flag tag epitope and nuclear localization signal driven by CMV promoter

Depositor

Charles Gersbach

Article

Pickar-Oliver et al Nat Biotechnol. 2019 Sep 23. pii: 10.103

Insert

EcoCasE

Use

CRISPR

Tags

Flag, NLS

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pLenti-LmoCas6-KRAB-2a-BlastR

Plasmid

#126492

Purpose

Encodes lentivirus expression of L. monocytogenes CRISPR Type I-B Flag-Cas6-KRAB-2a-BlastR driven by hUbC promoter

Depositor

Charles Gersbach

Article

Pickar-Oliver et al Nat Biotechnol. 2019 Sep 23. pii: 10.103

Insert

LmoCas6-KRAB-2a-BlastR

Use

CRISPR and Lentiviral

Tags

Flag, NLS

Expression

Mammalian

Mutation

Promoter

hUbC

Available since

Sept. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA-Flag-EcoCas8e

Plasmid

#106270

Purpose

Encodes human codon optimized E.coli CRISPR Type I-E CasA with N-terminal fusion of Flag tag epitope and nuclear localization signal driven by CMV promoter

Depositor

Charles Gersbach

Article

Pickar-Oliver et al Nat Biotechnol. 2019 Sep 23. pii: 10.103

Insert

EcoCasA

Use

CRISPR

Tags

Flag, NLS

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA-Flag-EcoCse2

Plasmid

#106271

Purpose

Encodes human codon optimized E.coli CRISPR Type I-E CasB with N-terminal fusion of Flag tag epitope and nuclear localization signal driven by CMV promoter

Depositor

Charles Gersbach

Article

Pickar-Oliver et al Nat Biotechnol. 2019 Sep 23. pii: 10.103

Insert

EcoCasB

Use

CRISPR

Tags

Flag, NLS

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 30, 2019

Availability

Academic Institutions and Nonprofits only

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