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Plasmid#178062PurposeExpression of WLS-HA protein (Y392C mutant)DepositorInsertWLS (WLS Human)
UseTagsHA and IRES-GFPExpressionMammalianMutationY392CPromoterAvailable sinceJan. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-I531T-WLS-HA-IRES-GFP
Plasmid#178064PurposeExpression of WLS-HA protein (I531T mutant)DepositorInsertWLS (WLS Human)
UseTagsHA and IRES-GFPExpressionMammalianMutationI531TPromoterAvailable sinceJan. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
TRUPATH Triple GaZ
Plasmid#196053PurposeEncodes a G alpha subunit (GNAZ) with RLuc8, a G gamma subunit (GNG1) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteinsDepositorUseLuciferaseTagsGFP2 and GSAG linkerExpressionMammalianMutationRLuc8 and flanking SGGGGS linkers have been inser…PromoterCMVAvailable sinceMarch 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
TRUPATH Triple Ga15
Plasmid#196060PurposeEncodes a G alpha subunit (GNA15) with RLuc8, a G gamma subunit (GNG13) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteinsDepositorUseLuciferaseTagsGFP2 and GSAG linkerExpressionMammalianMutationRLuc8 and flanking SGGGGS linkers have been inser…PromoterCMVAvailable sinceFeb. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
TRUPATH Triple Gai2
Plasmid#196049PurposeEncodes a G alpha subunit (GNAl2) with RLuc8, a G gamma subunit (GNG8) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteinsDepositorUseLuciferaseTagsGFP2 and GSAG linkerExpressionMammalianMutationRLuc8 and flanking SGGGGS linkers have been inser…PromoterCMVAvailable sinceFeb. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
TRUPATH Triple Ga11
Plasmid#196059PurposeEncodes a G alpha subunit (GNA11) with RLuc8, a G gamma subunit (GNG13) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteinsDepositorUseLuciferaseTagsGFP2 and GSAG linkerExpressionMammalianMutationRLuc8 and flanking SGGGGS linkers have been inser…PromoterCMVAvailable sinceFeb. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
peGFP NOVA2 op
Plasmid#224354PurposeExpress GFP-tagged human NOVA2 (codon optimized)DepositorInsertNOVA2 (NOVA2 Human)
UseTagseGFPExpressionMammalianMutationcodon optimized for expression in human cellsPromoterCMV and T7Available sinceSept. 10, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pcDNA3_N-DYK_MTHFD1-NLS
Plasmid#133023PurposeNLS sequence was fused to MTHFD1-wild type through site-directed mutagenesis (Q5 Site-Directed Mutagenesis Kit, New England Biolabs)DepositorInsertMTHFD1 (MTHFD1 Human)
UseTagsNLSExpressionMammalianMutationPromoterCMVAvailable sinceAug. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3_N-DYK_MTHFD1-NES
Plasmid#133022PurposeNES sequence was fused to MTHFD1-wild type through site-directed mutagenesis (Q5 Site-Directed Mutagenesis Kit, New England Biolabs)DepositorInsertMTHFD1 (MTHFD1 Human)
UseTagsNESExpressionMammalianMutationPromoterCMVAvailable sinceAug. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 V5 hHR23B
Plasmid#13054DepositorInserthHR23B (RAD23B Human)
UseTagsHis and V5ExpressionMammalianMutationPromoterAvailable sinceOct. 5, 2006AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 V5 hHR23A
Plasmid#13053DepositorInserthHR23A (RAD23A Human)
UseTagsHis and V5ExpressionMammalianMutationPromoterAvailable sinceOct. 5, 2006AvailabilityAcademic Institutions and Nonprofits only -
GFP-A96 ELP
Plasmid#67856PurposeGFP Fluorescent Elastin-like Polypeptide(VPGAG) with 96 ELP repeats containing Alanine guest residueDepositorInsertGFP fused to ELP (VPGAG) repeated 96 times
UseTagsGFP fused to N-terminal of ELPExpressionMammalianMutationPromoterT7Available sinceOct. 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
GFP-I48 ELP
Plasmid#67857PurposeGFP Fluorescent Elastin-like Polypeptide(VPGIG) with 48 repeats containing Isoleucine guest residuesDepositorInsertGFP fused to ELP (VPGIG) repeated 48 times
UseTagsGFP fused to N-terminal of ELPExpressionMammalianMutationPromoterT7Available sinceOct. 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
CAGGS-AsCpf1(D908A)-2A-GFP-U6-sgRNA-cloning vector
Plasmid#194726PurposeCAGGS-AsCpf1(D908A)-2A-GFP-U6-sgRNA-cloning vectorDepositorArticleInsertAsCpf1(D908A)
UseCRISPRTagsExpressionMammalianMutationD908APromoterAvailable sinceJan. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
CAGGS-AsCpf1(D908A)-2A-GFP-U6-AAVS1-sgRNA
Plasmid#194727PurposeCAGGS-AsCpf1(D908A)-2A-GFP-U6-sgRNA-cloning vector, with guide RNA array targeting hAAVS1.DepositorArticleInsertAsCpf1(D908A) (AAVS1 Acidaminococcus sp.)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
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pcDNA3_2FLAG-AKAP79 WT
Plasmid#183450PurposeExpression of human wild-type AKAP79DepositorInsertAKAP5 (AKAP5 Human)
UseTags2xFLAGExpressionMammalianMutationPromoterCMVAvailable sinceJune 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
GFP-V96 ELP
Plasmid#67853PurposeGFP Fluorescent Elastin-like Polypeptide(VPGVG) with 96 repeats containing Valine guest residuesDepositorInsertGFP fused to ELP (VPGVG) repeated 96 times
UseTagsGFP fused to N-terminal of ELPExpressionMammalianMutationPromoterT7Available sinceOct. 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
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pcDNA3_2FLAG-AKAP79ΔPKA
Plasmid#183452PurposeExpression of human AKAP79 lacking the PKA binding site (Δ391-400)DepositorInsertAKAP5 (AKAP5 Human)
UseTags2xFLAGExpressionMammalianMutationaa391-400 deletedPromoterCMVAvailable sinceJune 21, 2022AvailabilityAcademic Institutions and Nonprofits only