Addgene

We narrowed to 8,628 results for: CAG

Showing: 4921 - 4940 of 8628 results

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MAP2K4 gRNA (BRDN0001145028)

Plasmid

#76650

Purpose

3rd generation lentiviral gRNA plasmid targeting human MAP2K4

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

MAP2K4 (MAP2K4 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pORANGE GFP-Arpc5 KI

Plasmid

#131503

Purpose

Endogenous tagging of Arp2/3 complex subunit 5: C-terminal (amino acid position: STOP codon)

Depositor

Harold MacGillavry

Article

Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

Insert

gRNA and GFP donor (Arpc5, Arpc5 Mouse, Rat)

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6 and Cbh

Available since

Sept. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pU6-pegRNA-HEK3-CTTins-px330-scaffold

Plasmid

#180017

Purpose

Transiently expressing a pegRNA to introduce HEK3 CTT insertion in human cells. It has a sgRNA scaffold from px330.

Depositor

Dirk Hockemeyer

Article

Li et al Elife. 2022 Sep 7;11:e79208. doi: 10.755

Insert

Prime editing pegRNA for HEK3-CTTins, with px330 scaffold (EPHA8 Synthetic)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Human U6

Available since

Sept. 8, 2022

Availability

Academic Institutions and Nonprofits only

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sgTP53(B)

Plasmid

#85560

Purpose

Expresses sgRNA targeting human TP53 around codon F109

Depositor

Luca Grumolato

Article

Guernet et al Mol Cell. 2016 Aug 4;63(3):526-38. doi:

Insert

tumor protein p53 (TP53 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 8, 2017

Availability

Academic Institutions and Nonprofits only
MSCV-Neo-GFP/Foxa1

Plasmid

#105506

Purpose

MSCV-driven retroviral Foxa1 expression

Depositor

Christopher Vakoc

Article

Roe et al Cell. 2017 Aug 24;170(5):875-888.e20. do

Insert

Foxa1 (Foxa1 Mouse)

Use

Retroviral

Tags

Expression

Mutation

Promoter

Available since

Feb. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pLKO.1-puro-shRFP

Plasmid

#125778

Purpose

constitutive expression of a short-hairpin RNA targeting RFP

Depositor

Francisca Vazquez

Article

Chan et al Nature. 2019 Apr 10. pii: 10.1038/s41586

Insert

shRFP

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

June 4, 2019

Availability

Academic Institutions and Nonprofits only

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pT2-shATRX

Plasmid

#124258

Purpose

Expresses shRNA targeting ATRX. Construct has inverted repeats to be used in Sleeping beauty system.

Depositor

Maria Castro

Article

Nunez et al Sci Transl Med. 2019 Feb 13;11(479). pii

Insert

shATRX

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 8, 2019

Availability

Academic Institutions and Nonprofits only

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shERK2a-mlpx-puro

Plasmid

#65229

Purpose

encodes a shRNA against ERK2

Depositor

Gerardo Ferbeyre

Article

Deschenes-Simard et al Genes Dev. 2013 Apr 15;27(8):900-15. doi

Insert

shRNA against ERK2 (MAPK1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 12, 2015

Availability

Academic Institutions and Nonprofits only

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pLKO.puro_shGLS

Plasmid

#110335

Purpose

shGLS (Target CAACTGGCCAAATTCAGTC), silence human GLS gene, puromycin selection

Depositor

Sandra Martha Gomes Dias

Article

Dias et al Oncogene. 2020 Jan;39(3):690-702. doi: 1

Insert

GLS glutaminase (GLS Synthetic, Human)

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

U6 (RNA PolIII)

Available since

Feb. 23, 2021

Availability

Academic Institutions and Nonprofits only

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CL20_mEGFP_SMARCA2_ZNF384

Plasmid

#205922

Purpose

Express mEGFP-tagged fusion protein, SMARCA2_ZNF384 from patient-derived sequence

Depositor

Richard Kriwacki

Article

Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

Insert

SMARCA2_ZNF384 (SMARCA2, ZNF384 Human)

Use

Tags

mEGFP

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

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MSCV-Neo-GFP/Flag-Gata5

Plasmid

#105508

Purpose

MSCV-driven retroviral Gata5 expression (Flag-tagged)

Depositor

Christopher Vakoc

Article

Roe et al Cell. 2017 Aug 24;170(5):875-888.e20. do

Insert

Gata5 (Gata5 Mouse)

Use

Retroviral

Tags

Flag

Expression

Mutation

Promoter

Available since

May 17, 2018

Availability

Academic Institutions and Nonprofits only

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AURKB gRNA (BRDN0001146837)

Plasmid

#77619

Purpose

3rd generation lentiviral gRNA plasmid targeting human AURKB

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

AURKB (AURKB Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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px335 Mettl14 sgRNA #1

Plasmid

#61515

Purpose

encodes sgRNA sequence for targeting mouse Mettl14 locus (Cas9-Nickase strategy)

Depositor

Jacob Hanna

Article

Geula et al Science. 2015 Feb 27;347(6225):1002-6. d

Insert

gcggcagctcctagctcagc

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 6, 2015

Availability

Academic Institutions and Nonprofits only
pCDNA3 ATP5O(TISU)-FF

Plasmid

#85489

Purpose

Firefly luciferase under the control of ATP5O TISU

Depositor

Ivan Topisirovic

Article

Gandin et al Genome Res. 2016 May;26(5):636-48. doi:

Insert

Full TISU element of ATP5O (ATP5PO Human)

Use

Luciferase

Tags

Firefly luciferase

Expression

Mammalian

Mutation

2nd and 3rd codon of luciferase were modified fro…

Promoter

Available since

Jan. 4, 2017

Availability

Academic Institutions and Nonprofits only

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pCDNA3 ATP5O(5’UTR)-SL-FF

Plasmid

#85491

Purpose

Firefly luciferase under the control of ATP5O 5'UTR followed by a stem-loop

Depositor

Ivan Topisirovic

Article

Gandin et al Genome Res. 2016 May;26(5):636-48. doi:

Insert

5'UTR of ATP5O followed by stem loop (ATP5PO Human)

Use

Luciferase

Tags

Firefly luciferase

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 13, 2017

Availability

Academic Institutions and Nonprofits only

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pLKO.1-TRC.mKO2_shGLS

Plasmid

#110319

Purpose

shGLS (Target CAACTGGCCAAATTCAGTC), silence human GLS gene and express monomeric Kusabira-Orange2

Depositor

Sandra Martha Gomes Dias

Article

Quintero et al BMC Cancer. 2017 Nov 7;17(1):727. doi: 1

Insert

GLS Glutaminase (GLS Human)

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

RNA polymerase III promoter for human U6 snRNA fo…

Available since

Nov. 6, 2018

Availability

Academic Institutions and Nonprofits only

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PX458-gR2A_ISO

Plasmid

#124808

Purpose

Vector for expression Cas9 with gRNA specific for rat IgG2a heavy chain locus. Use in combination with pHybr_r2a> plasmids to convert expression of rat IgG2a hybridomas to isotype of choice.

Depositor

Martijn Verdoes, Ferenc Scheeren

Article

van der Schoot et al Sci Adv. 2019 Aug 28;5(8):eaaw1822. doi:

Insert

hSPCas9

Use

Tags

3XFLAG and GFP

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 9, 2019

Availability

Academic Institutions and Nonprofits only

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MSCV-Neo-GFP/V5-Foxa1

Plasmid

#105507

Purpose

MSCV-driven retroviral Foxa1 expression (V5-tagged)

Depositor

Christopher Vakoc

Article

Roe et al Cell. 2017 Aug 24;170(5):875-888.e20. do

Insert

Foxa1 (Foxa1 Mouse)

Use

Retroviral

Tags

V5

Expression

Mutation

Promoter

Available since

Feb. 9, 2018

Availability

Academic Institutions and Nonprofits only
pLenti-multi-CRISPR-sgRipk1_#1-puro

Plasmid

#231984

Purpose

Knockout mouse Ripk1

Depositor

Brian Ruffell

Article

Hanggi et al Cancer Cell. 2024 Nov 13:S1535-6108(24)0

Insert

sgRNA with Cas9 with puromycin resistance (Ripk1 Mouse)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Feb. 19, 2025

Availability

Academic Institutions and Nonprofits only

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SpyTag-RBD Omicron XBB.1.5

Plasmid

#214724

Purpose

Expresses SARS-CoV-2 Omicron XBB.1.5 RBD (receptor-binding domain from Spike) with N-terminal SpyTag fusion in mammalian cells

Depositor

Mark Howarth

Article

Hills et al Nat Nanotechnol. 2024 May 6. doi: 10.103

Insert

Receptor-Binding Domain (S SARS-CoV-2)

Use

Tags

SpyTag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 17, 2024

Availability

Academic Institutions and Nonprofits only

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