Addgene

We narrowed to 5,051 results for: pcdna3.1

Showing: 4881 - 4900 of 5051 results

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Myc-Nix-S212A

Plasmid

#197514

Purpose

Mammalian expression of myc-tagged human Nix with an alanine substitution of serine-212. Based on Myc-Nix (#100795)

Depositor

Joseph Gordon

Article

da Silva Rosa et al Autophagy. 2021 Sep;17(9):2257-2272. doi

Insert

BCL2 interacting protein 3 like (BNIP3L Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

Serine 212 changed to alanine

Promoter

CMV

Available since

March 1, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV_GFP11-14-SEPT7

Plasmid

#180342

Purpose

mammalian expression of human SEPT7 fused to GFP11

Depositor

Manos Mavrakis

Article

Martins et al J Cell Biol. 2023 Mar 6;222(3):e20220301

Insert

SEPTIN7 (SEPTIN7 Human)

Use

Tags

GFP11

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV_SEPT9_i1-14-GFP11

Plasmid

#180344

Purpose

mammalian expression of human SEPT9_i1 fused to GFP11

Depositor

Manos Mavrakis

Article

Martins et al J Cell Biol. 2023 Mar 6;222(3):e20220301

Insert

SEPTIN9_v1 (SEPTIN9 Human)

Use

Tags

GFP11

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV_SEPT9_i3-14-GFP10

Plasmid

#180345

Purpose

mammalian expression of human SEPT9_i3 fused to GFP10

Depositor

Manos Mavrakis

Article

Martins et al J Cell Biol. 2023 Mar 6;222(3):e20220301

Insert

SEPTIN9_v3 (SEPTIN9 Human)

Use

Tags

GFP10

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV_SEPT9_i3-14-GFP11

Plasmid

#180346

Purpose

mammalian expression of human SEPT9_i3 fused to GFP11

Depositor

Manos Mavrakis

Article

Martins et al J Cell Biol. 2023 Mar 6;222(3):e20220301

Insert

SEPTIN9_v3 (SEPTIN9 Human)

Use

Tags

GFP11

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only

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Homer1L/S scFv [L113/130]

Plasmid

#190500

Purpose

Mammalian Expression of Homer1L/S scFV. Derived from hybridoma L113/130.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

Homer1L/S (Mus musculus) recombinant scFV (Homer1 Mouse)

Use

Tags

Sortase, 6xHis

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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NPY/Neuropeptide Y scFv [L115/13]

Plasmid

#190505

Purpose

Mammalian Expression of NPY/Neuropeptide Y scFV. Derived from hybridoma L115/13.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

NPY/Neuropeptide Y (Homo sapiens) recombinant scFV (NPY Mouse)

Use

Tags

HA, Sortase, 6xHis

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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Kv2.1 K+ channel scFv [K89/34]

Plasmid

#190494

Purpose

Mammalian Expression of Kv2.1 K+ channel scFV. Derived from hybridoma K89/34.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

Kv2.1 K+ channel (Rattus norvegicus) recombinant scFV (Kcnb1 Mouse)

Use

Tags

HA, Sortase, 6xHis

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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Kv1.1 K+ channel scFv [K20/78]

Plasmid

#190486

Purpose

Mammalian Expression of Kv1.1 K+ channel scFV. Derived from hybridoma K20/78.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

Kv1.1 K+ channel (Rattus norvegicus) recombinant scFV (Kcna1 Mouse)

Use

Tags

HA, Sortase, 6xHis

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
GABA(A)R, Gamma2L/S scFv [N452/73]

Plasmid

#190555

Purpose

Mammalian Expression of GABA(A)R, Gamma2L/S scFV. Derived from hybridoma N452/73.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

GABA(A)R, Gamma2L/S (Homo sapiens) recombinant scFV (GABRG2 Mouse)

Use

Tags

HA, Sortase, 6xHis

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
GABA(A)R, Gamma2L/S scFv [N452/81]

Plasmid

#190556

Purpose

Mammalian Expression of GABA(A)R, Gamma2L/S scFV. Derived from hybridoma N452/81.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

GABA(A)R, Gamma2L/S (Homo sapiens) recombinant scFV (GABRG2 Mouse)

Use

Tags

HA, Sortase, 6xHis

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-T7-NLS(SV40)-anCas(FCA)-6xHis-NLS(SV40)

Plasmid

#185701

Purpose

CMV and T7 promoter expression plasmid for human codon usage anCas(FCA) with an N-terminal NLS and C-terminal NLS

Depositor

Raul Perez-Jimenez

Article

Alonso-Lerma et al Nat Microbiol. 2023 Jan;8(1):77-90. doi:

Insert

anCas(FCA)

Use

Tags

6xHis-NLS and NLS

Expression

Mammalian

Mutation

optimized for human codon usage

Promoter

CMV and T7

Available since

June 7, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-T7-NLS(SV40)-anCas(PDCA)-6xHis-NLS(SV40)

Plasmid

#185705

Purpose

CMV and T7 promoter expression plasmid for human codon usage anCas(PDCA) with an N-terminal NLS and C-terminal NLS

Depositor

Raul Perez-Jimenez

Article

Alonso-Lerma et al Nat Microbiol. 2023 Jan;8(1):77-90. doi:

Insert

anCas(PDCA)

Use

Tags

6xHis-NLS and NLS

Expression

Mammalian

Mutation

optimized for human codon usage

Promoter

CMV and T7

Available since

June 7, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-T7-NLS(SV40)-anCas(SCA)-6xHis-NLS(SV40)

Plasmid

#185703

Purpose

CMV and T7 promoter expression plasmid for human codon usage anCas(SCA) with an N-terminal NLS and C-terminal NLS

Depositor

Raul Perez-Jimenez

Article

Alonso-Lerma et al Nat Microbiol. 2023 Jan;8(1):77-90. doi:

Insert

anCas(SCA)

Use

Tags

6xHis-NLS and NLS

Expression

Mammalian

Mutation

optimized for human codon usage

Promoter

CMV and T7

Available since

June 7, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-T7-NLS(SV40)-anCas(PCA)-6xHis-NLS(SV40)

Plasmid

#185704

Purpose

CMV and T7 promoter expression plasmid for human codon usage anCas(PCA) with an N-terminal NLS and C-terminal NLS

Depositor

Raul Perez-Jimenez

Article

Alonso-Lerma et al Nat Microbiol. 2023 Jan;8(1):77-90. doi:

Insert

anCas(PCA)

Use

Tags

6xHis-NLS and NLS

Expression

Mammalian

Mutation

optimized for human codon usage

Promoter

CMV and T7

Available since

June 7, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-T7-NLS(SV40)-anCas(BCA)-6xHis-NLS(SV40)

Plasmid

#185702

Purpose

CMV and T7 promoter expression plasmid for human codon usage anCas(BCA) with an N-terminal NLS and C-terminal NLS

Depositor

Raul Perez-Jimenez

Article

Alonso-Lerma et al Nat Microbiol. 2023 Jan;8(1):77-90. doi:

Insert

anCas(BCA)

Use

Tags

6xHis-NLS and NLS

Expression

Mammalian

Mutation

optimized for human codon usage

Promoter

CMV and T7

Available since

June 7, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
Kv1.2 K+ channel scFv [K14/16]

Plasmid

#182064

Purpose

Mammalian Expression of Kv1.2 K+ channel scFv. Derived from hybridoma K14/16.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

recombinant mouse scFv targeting Kv1.2 K+ channel (Homo sapiens) (KCNA2 Mouse)

Use

Tags

HA, His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 25, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3-hPC1_MTS_L4139P-eGFP-3HA

Plasmid

#108404

Purpose

Express putative mitochondrial targeting sequence of human PC1 from amino acid 4129 to 4157 with a likely pathogenic point mutation.

Depositor

Gregory Germino

Article

Lin et al Sci Rep. 2018 Feb 9;8(1):2743. doi: 10.1

Insert

PKD1 (PKD1 Human)

Use

Tags

3xHA and eGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3-mPC1_4119-eGFP

Plasmid

#108394

Purpose

Genomic region, including intron 45, to express cytoplasmic tail mouse PC1 from amino acid 4119 to 4293

Depositor

Gregory Germino

Article

Lin et al Sci Rep. 2018 Feb 9;8(1):2743. doi: 10.1

Insert

PKD1 (PKD1 Mouse)

Use

Tags

eGFP

Expression

Mammalian

Mutation

variant ID rs13468401 missense variant R4237Q

Promoter

CMV

Available since

Aug. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3-hPC1_MTS_V4146I-eGFP

Plasmid

#108405

Purpose

Express putative mitochondrial targeting sequence of human PC1 from amino acid 4129 to 4157 with a likely non-pathogenic variant.

Depositor

Gregory Germino

Article

Lin et al Sci Rep. 2018 Feb 9;8(1):2743. doi: 10.1

Insert

PKD1 (PKD1 Mouse)

Use

Tags

3xHA and eGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 2, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge