Addgene

We narrowed to 39,352 results for: c-myc

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pcDNA3.1-Nup88 (551-741)

Plasmid

#64252

Purpose

Mammalian expression of C-terminal domain of Nup88

Depositor

Birthe Fahrenkrog

Article

Lussi et al Mol Biol Cell. 2011 Apr;22(7):1080-90. d

Insert

Nup88 (NUP88 Human)

Use

Tags

his and myc

Expression

Mammalian

Mutation

C-terminal domain (551-741)

Promoter

CMV

Available since

April 23, 2015

Availability

Academic Institutions and Nonprofits only
pPS835

Plasmid

#8933

Depositor

Pamela Silver

Article

Schlenstedt et al Proc Natl Acad Sci U S A 1995 Jan 3;92(1

Insert

GSP1 (GSP1 Budding Yeast)

Use

Tags

myc

Expression

Yeast

Mutation

G21V, stabilizes the GTP-bound form

Promoter

Available since

Aug. 19, 2005

Availability

Academic Institutions and Nonprofits only
pEGFP-N1-FUS/TLS-FLAGC

Plasmid

#60362

Purpose

Plasmid for expression of FLAG-GFP tagged human FUS/TLS (C-terminal tag). Confers resistance to G418.

Depositor

Sébastien Britton, Patrick Calsou

Article

Britton et al Nucleic Acids Res. 2014 Oct 1;42(14):904

Insert

FUS (FUS Human)

Use

Tags

FLAG and GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 8, 2015

Availability

Academic Institutions and Nonprofits only

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MSCV

Plasmid

#24828

Depositor

Lin He

Article

Olive et al Genes Dev. 2009 Dec 15. 23(24):2839-49.

Type

Empty backbone

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 10, 2010

Availability

Academic Institutions and Nonprofits only

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pMN20

Plasmid

#11573

Depositor

Detlef Weigel

Article

Weigel et al Plant Physiol. 2000 Apr . 122(4):1003-13

Insert

CaMV 35S enhancers

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

May 31, 2006

Availability

Academic Institutions and Nonprofits only

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pMN19

Plasmid

#11572

Depositor

Detlef Weigel

Article

Weigel et al Plant Physiol. 2000 Apr . 122(4):1003-13

Insert

CaMV 35S enhancers

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

May 31, 2006

Availability

Academic Institutions and Nonprofits only

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pCFJ2089 - [1-2] ENTR - Tag - SNAP(PATC, no_atg, no_stop)

Plasmid

#159874

Purpose

Three-fragment Gateway compatible protein tag for expression in C. elegans

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

[1-2] ENTR - Tag - SNAP(PATC, no_atg, no_stop)

Use

Tags

Expression

Worm

Mutation

Not applicable

Promoter

Available since

Dec. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pCFJ2211 - [1-2] ENTR - Fluor - mMaple3(intron, PATC, no_atg, no_stop)

Plasmid

#159866

Purpose

Three-fragment Gateway compatible flourophore for expression in C. elegans

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

[1-2] ENTR - Fluor - mMaple3(intron, PATC, no_atg, no_stop)

Use

Tags

Expression

Worm

Mutation

Not applicable

Promoter

Available since

Dec. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pHyg-CUbo(K48R/K63R)-His6

Plasmid

#212780

Purpose

C-terminal PCR-based tagging with CUbo(K48R/K63R)-His6 in budding yeast with HygR selection

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Type

Empty backbone

Use

Pcr-based tagging

Tags

Expression

Mutation

Promoter

Available since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pHyg-CUbo(K48R/K63R)-yeGFP

Plasmid

#212769

Purpose

C-terminal PCR-based tagging with CUbo(K48R/K63R)-yeGFP in budding yeast with HygR selection

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Type

Empty backbone

Use

Pcr-based tagging

Tags

Expression

Mutation

Promoter

Available since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pHyg-CUbo(K48R)-yeGFP-His6

Plasmid

#212783

Purpose

C-terminal PCR-based tagging with CUbo(K48R)-yeGFP-His6 in budding yeast with HygR selection

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Type

Empty backbone

Use

Pcr-based tagging

Tags

Expression

Mutation

Promoter

Available since

Jan. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pHyg-CUbo(K63R)-yeGFP-His6

Plasmid

#212784

Purpose

C-terminal PCR-based tagging with CUbo(K63R)-yeGFP-His6 in budding yeast with HygR selection

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Type

Empty backbone

Use

Pcr-based tagging

Tags

Expression

Mutation

Promoter

Available since

Jan. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pCFJ1972 - [1-2] ENTR - Flour - TagBFP2(NLS(2), no_atg, no_stop)

Plasmid

#159844

Purpose

Three-fragment Gateway compatible flourophore for expression in C. elegans

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

[1-2] ENTR - Flour - TagBFP2(NLS(2), no_atg, no_stop)

Use

Tags

Expression

Worm

Mutation

Not applicable

Promoter

Available since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pBiT3.1-C_FBXW7:1-707

Plasmid

#211104

Purpose

Mammalian expression of full-length human FBXW7. C-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

FBXW7:M1-K707 (FBXW7 Human)

Use

Tags

GSSGGSSGVSGWRLFKKIS

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

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pBiT3.1-C_DDB1:1-1140

Plasmid

#211188

Purpose

Mammalian expression of full-length human DDB1. loop deletion amino acids I396-D689. C-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

DDB1:M1-H1140 (DDB1 Human)

Use

Tags

GSSGGSSGVSGWRLFKKIS

Expression

Mammalian

Mutation

loop deletion I396-D689

Promoter

CMV

Available since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

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pBiT3.1-C_LRRK2:2142-2527

Plasmid

#211151

Purpose

Mammalian expression of human LRRK2 fragment R2142 to E2527. C-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

LRRK2:R2142-E2527 (LRRK2 Human)

Use

Tags

GSSGGSSGVSGWRLFKKIS

Expression

Mammalian

Mutation

M2397T natural variant ( VAR_024965)

Promoter

CMV

Available since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

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pBiT3.1-C_EIF3B:186-685

Plasmid

#211279

Purpose

Mammalian expression of human EIF3B fragment V186 to T685. C-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

EIF3B:V186-T685 (EIF3B Human)

Use

Tags

GSSGGSSGVSGWRLFKKIS

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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PCDH-METTL14-R298P

Plasmid

#141113

Purpose

Overexpression of METTL14 mutant in mammalian cells.

Depositor

Jianjun Chen

Article

Weng et al Cell Stem Cell. 2018 Feb 1;22(2):191-205

Insert

HMETTL14 (METTL14 Human)

Use

Tags

Expression

Mammalian

Mutation

Substitution - Missense, position 298, R➞P

Promoter

Available since

June 26, 2020

Availability

Academic Institutions and Nonprofits only

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MSCV-PIG-METTL14-WT

Plasmid

#141118

Purpose

Retroviral overexpression of WT METTL14 in mammalian cells.

Depositor

Jianjun Chen

Article

Weng et al Cell Stem Cell. 2018 Feb 1;22(2):191-205

Insert

METTL14 (METTL14 Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

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MSCV-PIG-METTL14-R298P

Plasmid

#141119

Purpose

Retroviral overexpression of mutant METTL14 in mammalian cells.

Depositor

Jianjun Chen

Article

Weng et al Cell Stem Cell. 2018 Feb 1;22(2):191-205

Insert

METTL14 (METTL14 Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Substitution - Missense, position 298, R➞P

Promoter

Available since

June 26, 2020

Availability

Academic Institutions and Nonprofits only

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