Addgene

We narrowed to 6,544 results for: tev

Showing: 4841 - 4860 of 6544 results

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pGEX4T3-GST-PolB(K72A)

Plasmid

#177132

Purpose

Bacterial vector for expression of an N-terminal GST fusion of PolB(K72A) with a TEV protease site located between the GST tag and PolB(K72A)

Depositor

Robert Sobol

Article

Fang et al Nucleic Acids Res. 2019 Jul 9;47(12):626

Insert

PolB (POLB Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

Tac

Available since

July 6, 2022

Availability

Academic Institutions and Nonprofits only

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pJCC_162 SpCas9 KES(1107-1109)GG

Plasmid

#179526

Purpose

For bacterial expression of SpCas9 KES(1107-1109)GG (phosphate lock loop mutant) with an N-terminal His-MBP tag

Depositor

Jennifer Doudna

Article

Cofsky et al Nat Struct Mol Biol. 2022 Apr;29(4):395-

Insert

SpCas9 KES(1107-1109)GG

Use

Tags

10X His, TEV protease cleavage site, and maltose-…

Expression

Bacterial

Mutation

replaced KES (residues 1107-1109) with GG

Promoter

Available since

April 19, 2022

Availability

Academic Institutions and Nonprofits only

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pJCC_163 SpCas9 K233A/K234A/K253A/K263A

Plasmid

#179527

Purpose

For bacterial expression of SpCas9 K233A/K234A/K253A/K263A (helix-rolling basic patch mutant) with an N-terminal His-MBP tag

Depositor

Jennifer Doudna

Article

Cofsky et al Nat Struct Mol Biol. 2022 Apr;29(4):395-

Insert

SpCas9 K233A/K234A/K253A/K263A

Use

Tags

10X His, TEV protease cleavage site, and maltose-…

Expression

Bacterial

Mutation

changed lysines 233, 234, 253, and 263 to alanine

Promoter

Available since

April 19, 2022

Availability

Academic Institutions and Nonprofits only

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pGEX4T3-GST-PolB(K206A/K244A)

Plasmid

#177135

Purpose

Bacterial vector for expression of an N-terminal GST fusion of PolB(K206A/K244A) with a TEV protease site located between the GST tag and PolB(K206A/K244A)

Depositor

Robert Sobol

Article

Fang et al Nucleic Acids Res. 2019 Jul 9;47(12):626

Insert

PolB

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

Tac

Available since

Dec. 16, 2021

Availability

Academic Institutions and Nonprofits only

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EcRfaHKOW-L1L2NusG

Plasmid

#113126

Purpose

Expresses C-terminus of E. coli RfaH with L1 and L2 loops replaced with NusG residues

Depositor

James Berger

Article

Lawson et al Mol Cell. 2018 Sep 20;71(6):911-922.e4.

Insert

RfaH (rfaH Escherichia coli)

Use

Tags

His6-MBP-N10-TEV

Expression

Bacterial

Mutation

Deleted residues 1-107, replaced L1 and L2 loops …

Promoter

T7

Available since

Oct. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pCDF11-SFPQ(214-535)

Plasmid

#135439

Purpose

Expresses SFPQ 214-535 with TEV-cleavable hexahistidine tag

Depositor

Charles Bond

Article

Lee et al Nucleic Acids Res. 2015 Apr 20;43(7):382

Insert

splicing factor proline and glutamine rich (SFPQ Human)

Use

Tags

Hexahistidine

Expression

Bacterial

Mutation

Promoter

T7

Available since

May 15, 2020

Availability

Academic Institutions and Nonprofits only

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pDDGFP-Leu2d-GgKDELR2 R159K

Plasmid

#123626

Purpose

Expresses GgKDELR2 with Arg159Lys mutation in S. cerevisiae cells using Galactose induction, -URA and -Leu selection

Depositor

Simon Newstead

Article

Brauer et al Science. 2019 Mar 8;363(6431):1103-1107.

Insert

KDELR2 (KDELR2 Chicken)

Use

Tags

TEV-GFP-His

Expression

Yeast

Mutation

Arg159Lys

Promoter

Available since

May 20, 2019

Availability

Academic Institutions and Nonprofits only

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pDDGFP-Leu2d-GgKDELR2 H12A

Plasmid

#123619

Purpose

Expresses GgKDELR2 with His12Ala mutation in S. cerevisiae cells using Galactose induction, -URA and -Leu selection

Depositor

Simon Newstead

Article

Brauer et al Science. 2019 Mar 8;363(6431):1103-1107.

Insert

KDELR2 (KDELR2 Chicken)

Use

Tags

TEV-GFP-His

Expression

Yeast

Mutation

His12Ala

Promoter

Available since

May 20, 2019

Availability

Academic Institutions and Nonprofits only

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pDDGFP-Leu2d-GgKDELR2 R47A

Plasmid

#123620

Purpose

Expresses GgKDELR2 with Arg47Ala mutation in S. cerevisiae cells using Galactose induction, -URA and -Leu selection

Depositor

Simon Newstead

Article

Brauer et al Science. 2019 Mar 8;363(6431):1103-1107.

Insert

KDELR2 (KDELR2 Chicken)

Use

Tags

TEV-GFP-His

Expression

Yeast

Mutation

Arg47Ala

Promoter

Available since

May 20, 2019

Availability

Academic Institutions and Nonprofits only

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pDDGFP-Leu2d-GgKDELR2 R47K

Plasmid

#123621

Purpose

Expresses GgKDELR2 with Arg47Lys mutation in S. cerevisiae cells using Galactose induction, -URA and -Leu selection

Depositor

Simon Newstead

Article

Brauer et al Science. 2019 Mar 8;363(6431):1103-1107.

Insert

KDELR2 (KDELR2 Chicken)

Use

Tags

TEV-GFP-His

Expression

Yeast

Mutation

Arg47Lys

Promoter

Available since

May 20, 2019

Availability

Academic Institutions and Nonprofits only

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pDDGFP-Leu2d-GgKDELR2 E127A

Plasmid

#123622

Purpose

Expresses GgKDELR2 with Glu127Ala mutation in S. cerevisiae cells using Galactose induction, -URA and -Leu selection

Depositor

Simon Newstead

Article

Brauer et al Science. 2019 Mar 8;363(6431):1103-1107.

Insert

KDELR2 (KDELR2 Chicken)

Use

Tags

TEV-GFP-His

Expression

Yeast

Mutation

Glu127Ala

Promoter

Available since

May 20, 2019

Availability

Academic Institutions and Nonprofits only

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pDDGFP-Leu2d-GgKDELR2 E127Q

Plasmid

#123623

Purpose

Expresses GgKDELR2 with Glu127Gln mutation in S. cerevisiae cells using Galactose induction, -URA and -Leu selection

Depositor

Simon Newstead

Article

Brauer et al Science. 2019 Mar 8;363(6431):1103-1107.

Insert

KDELR2 (KDELR2 Chicken)

Use

Tags

TEV-GFP-His

Expression

Yeast

Mutation

Glu127Gln

Promoter

Available since

May 20, 2019

Availability

Academic Institutions and Nonprofits only

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pDDGFP-Leu2d-GgKDELR2 Y158F

Plasmid

#123624

Purpose

Expresses GgKDELR2 with Tyr158Phe mutation in S. cerevisiae cells using Galactose induction, -URA and -Leu selection

Depositor

Simon Newstead

Article

Brauer et al Science. 2019 Mar 8;363(6431):1103-1107.

Insert

KDELR2 (KDELR2 Chicken)

Use

Tags

TEV-GFP-His

Expression

Yeast

Mutation

Tyr158Phe

Promoter

Available since

May 20, 2019

Availability

Academic Institutions and Nonprofits only

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pDDGFP-Leu2d-GgKDELR2 R159A

Plasmid

#123625

Purpose

Expresses GgKDELR2 with Arg159Ala mutation in S. cerevisiae cells using Galactose induction, -URA and -Leu selection

Depositor

Simon Newstead

Article

Brauer et al Science. 2019 Mar 8;363(6431):1103-1107.

Insert

KDELR2 (KDELR2 Chicken)

Use

Tags

TEV-GFP-His

Expression

Yeast

Mutation

Arg159Ala

Promoter

Available since

May 20, 2019

Availability

Academic Institutions and Nonprofits only

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pLAP-BUBR1-3A

Plasmid

#114054

Purpose

transfection in mammalian cells, combination of fluorescent protein fusion and tandem affinity purification

Depositor

Geert Kops

Article

Suijkerbuijk et al Dev Cell. 2012 Oct 16;23(4):745-55. doi:

Insert

BUBR1-3A (BUB1B Human)

Use

Tags

YFP-TEV-S- Human LAP

Expression

Mammalian

Mutation

C2823A and G2826A (siRNA-resistance), BUBR1-S670A…

Promoter

Available since

Jan. 23, 2019

Availability

Academic Institutions and Nonprofits only

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pLAP-BUBR1-3D

Plasmid

#114055

Purpose

transfection in mammalian cells, combination of fluorescent protein fusion and tandem affinity purification

Depositor

Geert Kops

Article

Suijkerbuijk et al Dev Cell. 2012 Oct 16;23(4):745-55. doi:

Insert

BUBR1-3D (BUB1B Human)

Use

Tags

YFP-TEV-S- Human LAP

Expression

Mammalian

Mutation

C2823A and G2826A (siRNA-resistance), BUBR1-S670D…

Promoter

Available since

Jan. 23, 2019

Availability

Academic Institutions and Nonprofits only

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pLAP-MIS12-KARD-3D

Plasmid

#114058

Purpose

transfection in mammalian cells, combination of fluorescent protein fusion and tandem affinity purification

Depositor

Geert Kops

Article

Suijkerbuijk et al Dev Cell. 2012 Oct 16;23(4):745-55. doi:

Insert

MIS12-KARD-3D (BUB1B Human)

Use

Tags

YFP-TEV-S- Human LAP

Expression

Mammalian

Mutation

BUBR1-S670D, S676D and T680D

Promoter

Available since

Sept. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pLAP-MIS12-KARD-3A

Plasmid

#114059

Purpose

transfection in mammalian cells, combination of fluorescent protein fusion and tandem affinity purification

Depositor

Geert Kops

Article

Suijkerbuijk et al Dev Cell. 2012 Oct 16;23(4):745-55. doi:

Insert

MIS12-KARD-3A (BUB1B Human)

Use

Tags

YFP-TEV-S- Human LAP

Expression

Mammalian

Mutation

BUBR1-S670A, S676A and T680A

Promoter

Available since

Sept. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pJSC119 - Bacterial expression plasmid for SpCas9 + N497A/R661A/Q695A variant

Plasmid

#101207

Purpose

Bacterial expression plasmid for SpCas9 + N497A/R661A/Q695A variant

Depositor

Keith Joung, Jennifer Doudna

Article

Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

Insert

SpCas9 variant N497A/R661A/Q695A

Use

Tags

10x His, MBP, and TEV site

Expression

Bacterial

Mutation

N497A, R661A and Q695A

Promoter

T7

Available since

Nov. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pJSC052 - Bacterial expression plasmid for SpCas9, REC3 FRET variant

Plasmid

#101201

Purpose

Bacterial expression plasmid for SpCas9, REC3 FRET variant

Depositor

Keith Joung, Jennifer Doudna

Article

Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

Insert

SpCas9 variant C80S/C574S/S701C/S960C

Use

Tags

10x His, MBP, and TEV site

Expression

Bacterial

Mutation

C80S, C574S, S701C and S960C

Promoter

T7

Available since

Oct. 30, 2017

Availability

Academic Institutions and Nonprofits only

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