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Plasmid#196668PurposeExpression of C-terminally distrep tag II-mClover3-tagged WzyE from Pectobacterium atrosepticum in E.coliDepositorInsertWzyE
UseTagsTEV-cleavable di-strep tag II and mClover3ExpressionBacterialMutationPromoteraraBADAvailable sinceJuly 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28_human-pro-IL-18_DeltaN24
Plasmid#214327PurposeProtein overexpression in bacteriaDepositorInsertIL18 (IL18 Human)
UseTagsHis-TEVExpressionBacterialMutationDeletion of first 24 amino acidsPromoterT7Available sinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuet-1-Cdc45
Plasmid#208796PurposeXenopus laevis FLAG5-His10-TEV-Cdc45DepositorInsertCdc45 (cdc45.L Frog)
UseTagsFLAG and HisExpressionBacterialMutationPromoterT7Available sinceFeb. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
His-ATG3-K62D/K64D
Plasmid#202786PurposeATG3 mutant overexpression in E.Coli. 6His-TEVsite-ATG3-K62D/K64D-stopDepositorInsertATG3 (ATG3 Human)
UseTags6xHis, TEVExpressionBacterialMutationK62D, K64DPromoterAvailable sinceAug. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
His-ATG3-H262A
Plasmid#202782PurposeATG3 mutant overexpression in E.Coli. 6His-TEVsite-ATG3-H262A-stopDepositorInsertATG3 (ATG3 Human)
UseTags6xHis, TEVExpressionBacterialMutationH262APromoterAvailable sinceAug. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
His-ATG3-T244A
Plasmid#202781PurposeATG3 mutant overexpression in E.Coli. 6His-TEVsite-ATG3-T244A-stopDepositorInsertATG3 (ATG3 Human)
UseTags6xHis, TEVExpressionBacterialMutationT244APromoterAvailable sinceAug. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
His-ATG3-Y209A
Plasmid#202779PurposeATG3 mutant overexpression in E.Coli. 6His-TEVsite-ATG3-Y209A-stopDepositorInsertATG3 (ATG3 Human)
UseTags6xHis, TEVExpressionBacterialMutationY209APromoterAvailable sinceAug. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
His-ATG3-Y210A
Plasmid#202780PurposeATG3 mutant overexpression in E.Coli. 6His-TEVsite-ATG3-Y210A-stopDepositorInsertATG3 (ATG3 Human)
UseTags6xHis, TEVExpressionBacterialMutationY210APromoterAvailable sinceAug. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
His-ATG3-K208D
Plasmid#202785PurposeATG3 mutant overexpression in E.Coli. 6His-TEVsite-ATG3-K208D-stopDepositorInsertATG3 (ATG3 Human)
UseTags6xHis, TEVExpressionBacterialMutationK208DPromoterAvailable sinceJuly 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
His-ATG3-C264A
Plasmid#202778PurposeATG3 mutant overexpression in E.Coli. 6His-TEVsite-ATG3-C264A-stopDepositorInsertATG3 (ATG3 Human)
UseTags6xHis, TEVExpressionBacterialMutationC264APromoterAvailable sinceJuly 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
His-ATG3-R265A
Plasmid#202783PurposeATG3 mutant overexpression in E.Coli. 6His-TEVsite-ATG3-R265A-stopDepositorInsertATG3 (ATG3 Human)
UseTags6xHis, TEVExpressionBacterialMutationR265APromoterAvailable sinceJuly 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
His-ATG3-H266A
Plasmid#202784PurposeATG3 mutant overexpression in E.Coli. 6His-TEVsite-ATG3-H266A-stopDepositorInsertATG3 (ATG3 Human)
UseTags6xHis, TEVExpressionBacterialMutationH266APromoterAvailable sinceJuly 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
hTln1-R1R2
Plasmid#191440PurposeExpresses the human TLN1 R1R2 domains in bacteriaDepositorInsertHuman Talin1 R1R2 domains (TLN1 Human)
UseTagsHis-tag, TEV cleavage siteExpressionBacterialMutationPromoterAvailable sinceJune 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
gZiPro W5F
Plasmid#198443PurposeActivity/inhibition assays, 19F-NMR assignmentDepositorInsertglycine linked Zika virus NS2B-NS3
UseTagsHis6-TEVExpressionBacterialMutationR95*A, W5FPromoterT7 promoterAvailable sinceApril 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
gZiPro W89F
Plasmid#198447PurposeActivity/inhibition assays, 19F-NMR assignmentDepositorInsertglycine linked Zika virus NS2B-NS3
UseTagsHis6-TEVExpressionBacterialMutationR95*A, W89FPromoterT7 promoterAvailable sinceMarch 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
gZiPro W83F
Plasmid#198446PurposeActivity/inhibition assays, 19F-NMR assignmentDepositorInsertglycine linked Zika virus NS2B-NS3
UseTagsHis6-TEVExpressionBacterialMutationR95*A, W83FPromoterT7 promoterAvailable sinceMarch 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
gZiPro W69F
Plasmid#198445PurposeActivity/inhibition assays, 19F-NMR assignmentDepositorInsertglycine linked Zika virus NS2B-NS3
UseTagsHis6-TEVExpressionBacterialMutationR95*A, W69FPromoterT7 promoterAvailable sinceMarch 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
eZiPro
Plasmid#198434PurposeActivity/inhibition assays, 19F-NMRDepositorInsertenzymatically cleavable linked Zika virus NS2B-NS3
UseTagsHis6-TEVExpressionBacterialMutationPromoterT7 promoterAvailable sinceMarch 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
gZiPro W50F
Plasmid#198444PurposeActivity/inhibition assays, 19F-NMR assignmentDepositorInsertglycine linked Zika virus NS2B-NS3
UseTagsHis6-TEVExpressionBacterialMutationR95*A, W50FPromoterT7 promoterAvailable sinceMarch 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEX4T3-GST-PolB(T304I)
Plasmid#177133PurposeBacterial vector for expression of an N-terminal GST fusion of PolB(T304I) with a TEV protease site located between the GST tag and PolB(T304I)DepositorInsertPolB (POLB Human)
UseTagsGSTExpressionBacterialMutationPromoterTacAvailable sinceSept. 14, 2022AvailabilityAcademic Institutions and Nonprofits only