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Plasmid#175020PurposeExpresses influenza D virus trimeric hemagglutinin esterase fusion protein fused to a sfGFPDepositorInsertD/bovine/Nebraska/9-5/2012
UseTagsGCN4-TEV-sfGFP-TwinStrepExpressionMammalianMutations57a esterase knock out mutantPromoterCMVAvailable sinceJan. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSHS273 - Bacterial expression plasmid for SpCas9∆REC3 variant
Plasmid#101202PurposeBacterial expression plasmid for SpCas9∆REC3 variantDepositorInsertSpCas9 variant M1–N497,GGS,V713–D1368
UseTags10x His, MBP, and TEV siteExpressionBacterialMutationM1–N497, GGS, V713–D1368PromoterT7Available sinceOct. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
TIRAP-TAP_IRES_GFP
Plasmid#52740PurposeRetroviral vector expresses hTIRAP-3xHA and a target site for the biotin ligase BirA upstream of IRES and GFPDepositorInsertToll/IL-1 Receptor adaptor protein (TIRAP Human)
UseRetroviralTags3x HA, 3x TEV, and BirA target siteExpressionMammalianMutationPromoterAvailable sinceJuly 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
pJSC272 - Bacterial expression plasmid for SpCas9 Cluster 5 + Q926A variant
Plasmid#101236PurposeBacterial expression plasmid for SpCas9 Cluster 5 + Q926A variantDepositorInsertSpCas9 variant K918A/V922A/R925A/Q926A
UseTags10x His, MBP, and TEV siteExpressionBacterialMutationK918A, V922A, R925A and Q926APromoterT7Available sinceOct. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET-22HT-MSI1(7-103)
Plasmid#60357PurposeExpress the RRM1 of mouse MSI1 in bacterial cells for protein purificationDepositorInsertmouse MSI1 (7-103) (Msi1 Mouse)
UseTagsHis and TEV protease siteExpressionBacterialMutationcontains amino acids 7-103 of Msi1PromoterT7Available sinceNov. 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCDF11-SFPQ(369-598)
Plasmid#135438PurposeExpresses SFPQ 369-598 with TEV-cleavable hexahistidine tagDepositorInsertsplicing factor proline and glutamine rich (SFPQ Human)
UseTagsHexahistidineExpressionBacterialMutationPromoterT7Available sinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJSC236 - Bacterial expression plasmid for SpCas9 Cluster 4 + Q926A variant
Plasmid#101235PurposeBacterial expression plasmid for SpCas9 Cluster 4 + Q926A variantDepositorInsertSpCas9 variant F491A/M495A/T496A/N497A/Q926A
UseTags10x His, MBP, and TEV siteExpressionBacterialMutationF491A, M495A, T496A, N497A and Q926APromoterT7Available sinceNov. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJSC239 - Bacterial expression plasmid for SpCas9 Cluster 3 + Q926A variant
Plasmid#101234PurposeBacterial expression plasmid for SpCas9 Cluster 3 + Q926A variantDepositorInsertSpCas9 variant T657A/G658A/W659A/R661A/Q926A
UseTags10x His, MBP, and TEV siteExpressionBacterialMutationT657A, G658A, W659A, R661A and Q926APromoterT7Available sinceNov. 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJSC269 - Bacterial expression plasmid for SpCas9 Cluster 1 + Q926A variant
Plasmid#101220PurposeBacterial expression plasmid for SpCas9 Cluster 1 + Q926A variantDepositorInsertSpCas9 variant N692A/M694A/Q695A/H698A/Q926A
UseTags10x His, MBP, and TEV siteExpressionBacterialMutationN692A, M694A, Q695A, H698A and Q926APromoterT7Available sinceOct. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEXP5-NT-CuSeCat EE
Plasmid#42569DepositorInsertmutated calmodulin gene
UseTagsPolyhistidine (6×His) region, TEV recognition sit…ExpressionBacterialMutationchanged Phenylalanine 92 to Glutamine acid, Valin…PromoterT7Available sinceMarch 4, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET-22HT-MSI1(7-192)
Plasmid#60245PurposeExpress the RBD of mouse MSI1 in bacterial cells for protein purificationDepositorInsertmouse MSI1 (7-192) (Msi1 Mouse)
UseTagsHis and TEV protease siteExpressionBacterialMutationcontains aa7-192 of Msi1PromoterT7Available sinceNov. 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET-22HT-MSI1(7-192)R61E
Plasmid#60353PurposeR61E mutant of the mouse MSI1 RBDDepositorInsertmouse MSI1 (7-192) (Msi1 Mouse)
UseTagsHis and TEV protease siteExpressionBacterialMutationR61E; contains amino acids 7-192 of Msi1PromoterT7Available sinceOct. 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
pJExpress401-KDAC4 CD H976Y
Plasmid#224250PurposeExpresses human KDAC4 (HDAC4) catalytic domain H976Y in E. coliDepositorInsertKDAC4 (HDAC4 Human)
UseTagsTEV-cleavable His6ExpressionBacterialMutationOnly includes residues 648-1083 (catalytic domain…PromoterT5Available sinceOct. 22, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLV_TRE3G_hRTN3E-Halo
Plasmid#216252PurposeTet-inducible hRTN3E (long form) expression vector, expresses hRTN3E-TEV-Halo, Lentiviral vectorDepositorInsertreticulon-3 isoform e (RTN3 Human)
UseLentiviralTagsHaloTagExpressionMutationPromoterTRE3GAvailable sinceAug. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHis-WzzE
Plasmid#196661PurposeExpression of N-terminally His6-tagged WzzE from Pectobacterium atrosepticum in E.coliDepositorInsertWzzE
UseTagsTEV-cleavable His6ExpressionBacterialMutationPromoterT7Available sinceJuly 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGST-WzzE
Plasmid#196662PurposeExpression of N-terminally GST-tagged WzzE from Pectobacterium atrosepticum in E.coliDepositorInsertWzzE
UseTagsTEV-cleavable GSTExpressionBacterialMutationValine insertion at position 2.PromoterT7Available sinceJuly 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPglL-strep-mCherry
Plasmid#196664PurposeExpression of C-terminally distrep tag II-mCherry-tagged PglL from Neisseria meningitidis in E.coliDepositorInsertPglL
UseTagsTEV-cleavable distrep tag II and mCherryExpressionBacterialMutationPromoterT7Available sinceJuly 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPglL-strep-mClover3
Plasmid#196665PurposeExpression of C-terminally distrep tag II-mClover3-tagged PglL from Neisseria meningitidis in E.coliDepositorInsertPglL
UseTagsTEV-cleavable di-strep tag II and mClover3ExpressionBacterialMutationPromoteraraBADAvailable sinceJuly 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pWzyE-GST-distrepII
Plasmid#196666PurposeExpression of C-terminally GST-distrep tag II-tagged WzyE from Pectobacterium atrosepticum in E.coliDepositorInsertWzyE
UseTagsGST and TEV-cleavable di-strep tag IIExpressionBacterialMutationPromoterT7Available sinceJuly 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pWzyE-distrepII
Plasmid#196667PurposeExpression of C-terminally distrep tag II-tagged WzyE from Pectobacterium atrosepticum in E.coliDepositorInsertWzyE
UseTagsTEV-cleavable di-strep tag IIExpressionBacterialMutationPromoterT7Available sinceJuly 19, 2024AvailabilityAcademic Institutions and Nonprofits only