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pLKO.1-shGBP1.2.mKO2
Plasmid#85210PurposeTRCN0000116120 (Target TGAGACGACGAAAGGCATGTA) for silencing GBP1 gene and express monomeric Kusabira-Orange2.DepositorInsertGBP1 (guanylate binding protein 1) (GBP1 Human)
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterRNA polymerase III promoter for human U6 snRNA fo…Available sinceOct. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pWZLblasti-HA-MEK5 CBM-E
Plasmid#53169PurposeExpresses HA tagged human MEK5 L280AH281AM323AQ332A from blasticidin resistance retroviral vectorDepositorInsertMEK5 (MAP2K5 Human)
UseRetroviralTagsHAExpressionMammalianMutationL280A/H281A/M323A/Q332APromoterAvailable sinceSept. 19, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBABEpuro-HA-MEK5 CBM-E
Plasmid#53199PurposeExpresses HA tagged human MEK5 L280A/H281A/M323A/Q332A from puromycin resistance retroviral vectorDepositorInsertMEK5 (MAP2K5 Human)
UseRetroviralTagsHAExpressionMammalianMutationL280A/H281A/M323A/Q332APromoterAvailable sinceSept. 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
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Mode #2 Library (14-3-3σ)
Pooled Library#111706PurposeEncodes 14-3-3σ and human phosphopeptides fused to split mCherry for Hi-PDepositorExpressionBacterialSpeciesHomo sapiensAvailable sinceDec. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
Mode #2 Library (14-3-3β)
Pooled Library#111705PurposeEncodes 14-3-3β and human phosphopeptides fused to split mCherry for Hi-PDepositorExpressionBacterialSpeciesHomo sapiensAvailable sinceDec. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
Mode #1 Library
Pooled Library#111704PurposeEncodes GST-fused human phosphopeptidesDepositorExpressionBacterialSpeciesHomo sapiensAvailable sinceDec. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV-GenEPi
Plasmid#140236PurposeMammalian expression of Piezo1-based fluorescent force sensor GenEPiDepositorInsertGenEPi (Piezo1-based fluorescent force sensor) (PIEZO1 Human)
UseTagsGCaMP 6s RS1 EF4ExpressionMammalianMutationPromotersimian CMV IE94Available sinceJuly 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCS2+MLS-HyPer7
Plasmid#136470PurposeMammalian expression of mitochondrial matrix targeted ultrasensitive hydrogen peroxide indicator HyPer7 for optical imagingDepositorInsertHyPer7
UseTagsTandem mitochondrial targeting signal of cytochro…ExpressionMammalianMutationPromoterCMV, SP6Available sinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAVS1-NC-CRISPRi (Gen3)
Plasmid#73499PurposeConstitutive CRISPR interference (CRISPRi) knock in construct into the AAVS1 locusDepositorInsertdCas9-KRAB
UseCRISPRTagsHA, KRAB, and NLSExpressionMammalianMutationD10A, H840A (catalytically deactivated Cas9)PromoterCAGAvailable sinceDec. 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
CamKII-LGI1-pHluorin
Plasmid#185537PurposeExpresses LGI1-pHluorin under the CamKII promoterDepositorInsertLGI1 (Lgi1 Rat)
UseLentiviralTagspHluorinExpressionMammalianMutationPromoterCamKIIAvailable sinceMay 3, 2024AvailabilityAcademic Institutions and Nonprofits only
