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pMAL-c5X-Dros16

Plasmid

#67004

Purpose

Expresses malE-Dros16 in E. coli

Depositor

Ashutosh Chilkoti

Article

Tang et al Nat Mater. 2016 Jan 4. doi: 10.1038/nmat

Insert

malE-Dros16

Use

Tags

His6 and malE-Factor Xa

Expression

Bacterial

Mutation

Promoter

tac

Available since

Jan. 5, 2016

Availability

Academic Institutions and Nonprofits only

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pGBW-m4133669

Plasmid

#152930

Purpose

Mammalian expression plasmid for SARS-CoV-2 ORF3a protein

Depositor

Benjie Chen, Ginkgo Bioworks

Insert

SARS-CoV-2 ORF3a protein (ORF3a Severe acute respiratory syndrome coronavirus 2)

Use

Tags

Cleavable TEV;6xHis-003

Expression

Mammalian

Mutation

wt

Promoter

CMV

Available since

July 30, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pGBW-m4133532

Plasmid

#152151

Purpose

Mammalian expression plasmid for SARS-CoV-2 ORF8 protein

Depositor

Benjie Chen, Ginkgo Bioworks

Insert

SARS-CoV-2 ORF8 protein (ORF8 Severe acute respiratory syndrome coronavirus 2)

Use

Tags

Cleavable TEV;6xHis-003

Expression

Mammalian

Mutation

wt

Promoter

CMV

Available since

July 30, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pGBW-m4133668

Plasmid

#153800

Purpose

Mammalian expression plasmid for SARS-CoV-2 E (envelope)

Depositor

Benjie Chen, Ginkgo Bioworks

Insert

SARS-CoV-2 E (envelope) (E Severe acute respiratory syndrome coronavirus 2)

Use

Tags

Cleavable TEV;6xHis-003

Expression

Mammalian

Mutation

wt

Promoter

CMV

Available since

June 12, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pGBW-m4133249

Plasmid

#152128

Purpose

Mammalian expression plasmid for SARS-CoV-2 ORF8 protein

Depositor

Benjie Chen, Ginkgo Bioworks

Insert

SARS-CoV-2 ORF8 protein (ORF8 Severe acute respiratory syndrome coronavirus 2)

Use

Tags

Cleavable TEV;6xHis-003

Expression

Mammalian

Mutation

wt

Promoter

CMV

Available since

May 20, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pGBW-m4258315

Plasmid

#154809

Purpose

Mammalian expression plasmid for SARS-CoV-2 ORF7b

Depositor

Benjie Chen, Ginkgo Bioworks

Insert

SARS-CoV-2 ORF7b (ORF7b Severe acute respiratory syndrome coronavirus 2)

Use

Tags

Cleavable TEV;V5

Expression

Mammalian

Mutation

wt

Promoter

CMV

Available since

July 30, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pGBW-m4134218

Plasmid

#152588

Purpose

Mammalian expression plasmid for SARS-CoV-2 ORF8 protein

Depositor

Benjie Chen, Ginkgo Bioworks

Insert

SARS-CoV-2 ORF8 protein (ORF8 Severe acute respiratory syndrome coronavirus 2)

Use

Tags

Cleavable TEV;CBP

Expression

Mammalian

Mutation

wt

Promoter

CMV

Available since

July 30, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pGBW-m4133586

Plasmid

#152328

Purpose

Mammalian expression plasmid for SARS-CoV-2 3C-like proteinase

Depositor

Benjie Chen, Ginkgo Bioworks

Insert

SARS-CoV-2 3C-like proteinase (ORF1ab Severe acute respiratory syndrome coronavirus 2)

Use

Tags

Cleavable TEV;6xHis-003

Expression

Mammalian

Mutation

codon optimized

Promoter

CMV

Available since

July 30, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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Tol2-ftyrp>TRAP

Plasmid

#42299

Depositor

Stephen L Johnson

Article

Tryon et al Genesis. 2012 Dec 19. doi: 10.1002/dvg.2

Insert

EGFP-Rpl10a (rpl10a Zebrafish)

Use

Tags

EGFP

Expression

Mutation

Promoter

Takifugu rubripes tyrosine related protein 1 (tyr…

Available since

March 7, 2013

Availability

Academic Institutions and Nonprofits only

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pGBW-m4252808

Plasmid

#153634

Purpose

Mammalian expression plasmid for SARS-CoV-2 nsp11

Depositor

Benjie Chen, Ginkgo Bioworks

Insert

SARS-CoV-2 nsp11 (ORF1ab Severe acute respiratory syndrome coronavirus 2)

Use

Tags

Cleavable TEV;3xFlag

Expression

Mammalian

Mutation

wt

Promoter

CMV

Available since

June 12, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pMLS297

Plasmid

#73737

Purpose

SapTrap STOP codon + let-858 UTR N-terminal connector donor plasmid

Depositor

Erik Jorgensen

Article

Schwartz et al Genetics. 2016 Feb 2. pii: genetics.115.

Insert

STOP codon + let-858 3'-UTR

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

March 25, 2016

Availability

Academic Institutions and Nonprofits only

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pGBW-m4133244

Plasmid

#153695

Purpose

Mammalian expression plasmid for SARS-CoV-2 E (envelope)

Depositor

Benjie Chen, Ginkgo Bioworks

Insert

SARS-CoV-2 E (envelope) (E Severe acute respiratory syndrome coronavirus 2)

Use

Tags

Cleavable TEV;6xHis-003

Expression

Mammalian

Mutation

wt

Promoter

CMV

Available since

June 12, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pCri-7b

Plasmid

#61330

Purpose

Protein expression.

Depositor

F Xavier Gomis-Ruth

Article

Goulas et al PLoS One. 2014 Nov 11;9(11):e112643. doi

Insert

Green fluorescent protein

Use

Tags

His-tag

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 9, 2015

Availability

Academic Institutions and Nonprofits only
pGBW-m4134312

Plasmid

#152550

Purpose

Mammalian expression plasmid for SARS-CoV-2 N (nucleocapsid)

Depositor

Benjie Chen, Ginkgo Bioworks

Insert

SARS-CoV-2 N (nucleocapsid) (N Severe acute respiratory syndrome coronavirus 2)

Use

Tags

Cleavable TEV;CBP

Expression

Mammalian

Mutation

wt

Promoter

CMV

Available since

July 30, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pGBW-m4046495

Plasmid

#148983

Purpose

Yeast Expression plasmid for SARS-CoV-2 nsp8

Depositor

Benjie Chen, Ginkgo Bioworks

Insert

SARS-CoV-2 nsp8 (ORF1ab Severe acute respiratory syndrome coronavirus 2, Budding Yeast)

Use

Tags

Cleavable thrombin;MBP

Expression

Yeast

Mutation

wt

Promoter

pTEF1

Available since

May 14, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pIBG-FTet

Plasmid

#33361

Depositor

Doug Mortlock

Article

Chandler et al Mol Cell Biol. 2007 Apr;27(8):2934-51. E

Inserts

IRES-Bgeo
TetR cassette

Use

Mouse Targeting

Tags

FRT site

Expression

Mutation

Promoter

Available since

Feb. 22, 2012

Availability

Academic Institutions and Nonprofits only

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pLNC Wnt-5bHA

Plasmid

#18034

Depositor

Jan Kitajewski

Article

Shimizu et al Cell Growth Differ. 1997 Dec . 8(12):134

Insert

Wnt-5b (Wnt5b Mouse)

Use

Retroviral

Tags

HA

Expression

Mutation

Promoter

Available since

July 7, 2008

Availability

Academic Institutions and Nonprofits only
pGBW-m4252823

Plasmid

#153623

Purpose

Mammalian expression plasmid for SARS-CoV-2 ORF6 protein

Depositor

Benjie Chen, Ginkgo Bioworks

Insert

SARS-CoV-2 ORF6 protein (ORF6 Severe acute respiratory syndrome coronavirus 2)

Use

Tags

Cleavable TEV;3xFlag

Expression

Mammalian

Mutation

wt

Promoter

CMV

Available since

June 12, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pGBW-m4252859

Plasmid

#153613

Purpose

Mammalian expression plasmid for SARS-CoV-2 ORF7b

Depositor

Benjie Chen, Ginkgo Bioworks

Insert

SARS-CoV-2 ORF7b (ORF7b Severe acute respiratory syndrome coronavirus 2)

Use

Tags

Cleavable TEV;3xFlag

Expression

Mammalian

Mutation

wt

Promoter

CMV

Available since

June 12, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pTHSSd_34

Plasmid

#59960

Purpose

PTac GFP expression

Depositor

Christopher Voigt

Article

Segall-Shapiro et al Mol Syst Biol. 2014 Jul 30;10(7):742.

Insert

sfGFP

Use

Tags

Expression

Bacterial

Mutation

N/A

Promoter

PTac

Available since

Sept. 17, 2014

Availability

Academic Institutions and Nonprofits only

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