-
Plasmid#86384PurposeVector to measure enhancer responsiveness of candidates from a genomic library (cloned instead of the core promoter) by determining the abundance of transcripts originating from each candidate.DepositorInsertD. melanogaster sgl enhancer
UseStap-seq screening vectorTagsExpressionMutationPromoterAvailable sinceMarch 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSTAP-seq_fly-ncm
Plasmid#86385PurposeVector to measure enhancer responsiveness of candidates from a genomic library (cloned instead of the core promoter) by determining the abundance of transcripts originating from each candidate.DepositorInsertD. melanogaster ncm enhancer
UseStap-seq screening vectorTagsExpressionMutationPromoterAvailable sinceMarch 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSTAP-seq_fly-zfh1_spikeIn-2
Plasmid#86388PurposeExpression vector for a transcript origination from a D. pse core promoter, which is used as trasfection control (normalization), i.e. STAP-seq screening vector containing one specific core promoterDepositorInsertD. melanogaster zfh1 enhancer & D. pseudoobscura CG32369 core promoter
UseStap-seq spike in vectorTagsExpressionMutationPromoterAvailable sinceMarch 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSTARR-seq_fly-x16
Plasmid#71505PurposeflySTARR-seq screening vector with x16 core promoterDepositorTypeEmpty backboneUseFlystarr-seq screening vectorTagssgGFP (SuperGlo, Qbiogene, Inc)ExpressionInsectMutationPromoterAvailable sinceFeb. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSTARR-seq_fly-eEF1delta
Plasmid#71501PurposeflySTARR-seq screening vector with eEF1delta core promoterDepositorTypeEmpty backboneUseFlystarr-seq screening vectorTagssgGFP (SuperGlo, Qbiogene, Inc)ExpressionInsectMutationPromoterAvailable sinceDec. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
Notothenia angustata cryaa
Plasmid#47666PurposeRecombinant protein productionDepositorInsertAlpha A-crystallin
UseTagsExpressionBacterialMutationPromoterT7Available sinceOct. 16, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGSTag BimL(AAA)
Plasmid#25063DepositorInsertBimL (AAA) (BCL2L11 Human)
UseTagsGstExpressionBacterialMutationChanged serine 44 to alanine Changed threonine 5…PromoterAvailable sinceAug. 13, 2010AvailabilityAcademic Institutions and Nonprofits only -
pGSTag BimL (S44A)
Plasmid#24252DepositorInsertBimL(S44A) (BCL2L11 Human)
UseTagsGstExpressionBacterialMutationChanged Serine 44 to AlaninePromoterAvailable sinceJune 1, 2010AvailabilityAcademic Institutions and Nonprofits only -
pGSTag BimL (T56A)
Plasmid#24253DepositorInsertBimL(T56A) (BCL2L11 Human)
UseTagsGSTExpressionBacterialMutationChanged threonine 56 to alaninePromoterAvailable sinceJune 1, 2010AvailabilityAcademic Institutions and Nonprofits only -
pGSTag BimL (S58A)
Plasmid#24254DepositorInsertBimL(S58A) (BCL2L11 Human)
UseTagsGstExpressionBacterialMutationChanged serine 58 to alaninePromoterAvailable sinceJune 1, 2010AvailabilityAcademic Institutions and Nonprofits only -
pGSTag BimEL (3SA)
Plasmid#23100DepositorInsertBim EL (3SA) (Bcl2l11 Mouse)
UseTagsGSTExpressionBacterialMutationchanged Serine 55/65/73 to alaninePromoterAvailable sinceFeb. 24, 2010AvailabilityAcademic Institutions and Nonprofits only -
qTAG-C-Solo-mStayGold-VIM
Plasmid#227302PurposeDonor template for mStayGold insertion into the C-terminus of the VIM locus. For intermediate filament visualization. To be co-transfected with sgRNA plasmid px330-PITCh-VIM (Addgene #227301)DepositorInsertVIM Homology Arms flanking a mStayGold Tag (VIM Human)
UseCRISPR; Donor templateTagsExpressionMammalianMutationPromoterPromoterlessAvailable sinceNov. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET21a(+)-Histag-mCherry (527)
Plasmid#70719PurposeExpression vector for mCherry purificationDepositorInsertmCherry
UseTagsExpressionMutationPromoterAvailable sinceFeb. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-10His-mStayGold (E138D)
Plasmid#211363PurposeMammalian expression of the bright and photostable monomeric StayGold fluorescent protein (E138D). Contains a N-terminal 10xHis tag.DepositorInsertmStayGold
UseTags10xHis-tagExpressionMammalianMutationE138DPromoterCMVAvailable sinceDec. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
siRNA-resistant SigmaR1-mCherry
Plasmid#226567PurposeEncodes siRNA resistant SigmaR1 protein labeled with mCherryDepositorInsertSigmaR1 (Sigma-1 Receptor) (SIGMAR1 Human)
UseTagsmCherryExpressionMammalianMutationPromoterAvailable sinceNov. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
His-CL7_MBP_1xNLS_iGeoCas9(C2)_2xNLS(star)
Plasmid#222991PurposeBacterial expression of iGeoCas9(C2) constructDepositorInsertHis-CL7_MBP_1xNLS_iGeoCas9(C2)_2xNLS(star)
UseCRISPRTagsCL7, His, and MBPExpressionBacterialMutationPromotertacAvailable sinceJuly 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-smURFP-StagRFP-EKAR-EV
Plasmid#173019PurposeRed/far-red FRET-based ERK activity reporter for multiplexed imaging applications.DepositorInsertsmURFP-StagRFP-EKAR-EV
UseTags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianMutationPromoterCMVAvailable sinceOct. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUASTattB-2xFlag-mCherry-Msp300KASH
Plasmid#170807PurposeExpresses Msp300 KASH domain fused to 2xFLAG and mCherry under Gal4/UAS control for Drosophila transgenesisDepositorInsert2xFLAG/mCherry-Msp300KASH
UseTags2xFLAG-mCherry and Msp300KASHExpressionInsectMutationPromoter5xUAS + hsp70 minimal promoterAvailable sinceSept. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
Stat3-C Flag pRc/CMV
Plasmid#8722DepositorInsertStat3-C (Stat3 Mouse)
UseTagsFlagExpressionMammalianMutationA662C and N664C. Residues changed to Cys renders…PromoterAvailable sinceSept. 26, 2005AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3puro-mGFP-FXR1a-sh5Resistant
Plasmid#225448PurposeExpress mEGFP-fusion protein of FXR1 isoform a with shRNA5-resistant silent mutationDepositorInsertFXR1 isoform a (FXR1 Human)
UseTagsmEGFPExpressionMammalianMutationPromoterCMVAvailable sinceOct. 10, 2024AvailabilityAcademic Institutions and Nonprofits only