Skip to main content
Addgene
Showing: 461 - 480 of 489 results

  2. pLVX-mCherry-KATNA1, V55D

    Plasmid
    #180651
    Purpose
    Lentiviral expression vector for KATNA1. Used for generating cell lines. Has N-terminal mCherry tag. Has V55D mutation. Dox-inducible. Internal ID: WISP20-48.
    Depositor
    Wesley Sundquist
    Article
    Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75
    Insert
    KATNA1 (KATNA1 Human)
    Use
    Lentiviral
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    siRNA resistant to: GGACAGCACUCCCUUGAAA
    Promoter
    Available since
    July 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    180651-plasmid-map-sequence-id-358612

    Enlarge

  3. pCA528 CHMP4C, residues 156-233, no Cys

    Plasmid
    #180642
    Purpose
    Bacterial expression for CHMP4C C-terminal tail, residues 156-233. Used for FP competition experiments. Has N-terminal HIS-SUMO tag. Internal ID: WISP20-14.
    Depositor
    Wesley Sundquist
    Article
    Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75
    Insert
    CHMP4C (CHMP4C Human)
    Use
    Tags
    HIS-SUMO
    Expression
    Bacterial
    Mutation
    residues 316-366
    Promoter
    T7
    Available since
    July 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    180642-plasmid-map-sequence-id-358646

    Enlarge

  4. pCA528 IST1, residues 315-360, no Cys

    Plasmid
    #180643
    Purpose
    Bacterial expression of IST1 C-terminal tail, residues 315-360. Used for FP competition experiments. Has N-terminal HIS-SUMO tag. Internal ID: WISP20-99.
    Depositor
    Wesley Sundquist
    Article
    Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75
    Insert
    IST1 (IST1 Human)
    Use
    Tags
    HIS-SUMO
    Expression
    Bacterial
    Mutation
    residues 315-360
    Promoter
    T7
    Available since
    July 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    180643-plasmid-map-sequence-id-358585

    Enlarge

  6. TFORF0467

    Plasmid
    #141619
    Purpose
    Lentiviral vector for overexpressing the CARHSP1 transcription factor ORF with a unique 24-bp barcode. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    CARHSP1 (CARHSP1 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Jan. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    141619-plasmid-map-sequence-id-391531

    Enlarge

  7. pIR-CMV-SCN1A-Variant-1-IRES-mScarlet

    Plasmid
    #162278
    Purpose
    Eukaryotic expression of human SCN1A variant 1 isoform. This channel has been modified to be stably maintained in standard bacterial strains.
    Depositor
    Al George
    Article
    DeKeyser et al J Biol Chem. 2021 Jan-Jun;296:100298. do
    Insert
    SCN1A Variant 1, stabilized (SCN1A Human)
    Use
    Tags
    IRES-mScarlet
    Expression
    Mammalian
    Mutation
    IVS intron inserted after bp2946. Silent codon c…
    Promoter
    CMV
    Available since
    July 20, 2021
    Availability
    Academic Institutions and Nonprofits only
    162278-plasmid-map-sequence-id-338003

    Enlarge

  14. pCAG-OSF-PP-ULK1(MIT)2, residues 833-1050

    Plasmid
    #180644
    Purpose
    Mammalian expression vector for ULK1 MIT domains, residues 833-1050. Has Precision protease cleavable N-terminal One-Strep Flag tag. Internal ID: WISP20-37.
    Depositor
    Wesley Sundquist
    Article
    Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75
    Insert
    ULK1 (ULK1 Human)
    Use
    Tags
    One-Strep-Flag (OSF)
    Expression
    Mammalian
    Mutation
    Residues 833-1050
    Promoter
    pCAG
    Available since
    July 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    180644-plasmid-map-sequence-id-358523

    Enlarge

  15. pCA528 CHMP4B, residues 156-224, no Cys

    Plasmid
    #180641
    Purpose
    Bacterial expression for CHMP4B C-terminal tail, residues 156-224. Used for FP competition experiments. Has N-terminal HIS-SUMO tag. Internal ID: WISP20-140
    Depositor
    Wesley Sundquist
    Article
    Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75
    Insert
    CHMP4B (CHMP4B Human)
    Use
    Tags
    HIS-SUMO
    Expression
    Bacterial
    Mutation
    Residues 156-224
    Promoter
    T7
    Available since
    July 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    180641-plasmid-map-sequence-id-358633

    Enlarge

  16. TFORF1134

    Plasmid
    #143468
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    CARF (ALS2CR8 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    June 22, 2023
    Availability
    Academic Institutions and Nonprofits only
    143468-plasmid-map-sequence-id-397893

    Enlarge

  17. TFORF1135

    Plasmid
    #143469
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    CARF (ALS2CR8 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    June 22, 2023
    Availability
    Academic Institutions and Nonprofits only
    143469-plasmid-map-sequence-id-397881

    Enlarge

  18. TFORF1133

    Plasmid
    #142734
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    CARF (ALS2CR8 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    June 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    142734-plasmid-map-sequence-id-395811

    Enlarge

Showing: 461 - 480 of 489 results