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Showing: 461 - 480 of 613 results

  1. pDRF'- AmTryoshka1;3-LS-L255I

    Plasmid
    #100964
    Purpose
    Yeast expression of fluorescent sensor reporting the activity of ammonium transceptors. Contains a stable reference LSSmOrange nested within the reporting superfolder cpEGFP.
    Depositor
    Wolf Frommer
    Article
    Ast et al Nat Commun. 2017 Sep 5;8(1):431. doi: 10
    Insert
    AmTryoshka1;3-LS-L255I (AMT1;3 Mustard Weed)
    Use
    Tags
    Expression
    Yeast
    Mutation
    L255I
    Promoter
    PMA
    Available since
    Oct. 3, 2017
    Availability
    Academic Institutions and Nonprofits only
    100964-plasmid-map-sequence-id-193747

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  2. pDRF'- AmTryoshka1;3-GS-F138I

    Plasmid
    #100965
    Purpose
    Yeast expression of fluorescent sensor reporting the activity of ammonium transceptors. Contains a stable reference LSSmOrange nested within the reporting superfolder cpEGFP.
    Depositor
    Wolf Frommer
    Article
    Ast et al Nat Commun. 2017 Sep 5;8(1):431. doi: 10
    Insert
    AmTryoshka1;3-GS-F138I (AMT1;3 Mustard Weed)
    Use
    Tags
    Expression
    Yeast
    Mutation
    F138I
    Promoter
    PMA
    Available since
    Oct. 3, 2017
    Availability
    Academic Institutions and Nonprofits only

  3. pDRF'- AmTryoshka1;3-GS-L255I

    Plasmid
    #100966
    Purpose
    Yeast expression of fluorescent sensor reporting the activity of ammonium transceptors. Contains a stable reference LSSmOrange nested within the reporting superfolder cpEGFP.
    Depositor
    Wolf Frommer
    Article
    Ast et al Nat Commun. 2017 Sep 5;8(1):431. doi: 10
    Insert
    AmTryoshka1;3-GS-L255I (AMT1;3 Mustard Weed)
    Use
    Tags
    Expression
    Yeast
    Mutation
    L255I
    Promoter
    PMA
    Available since
    Oct. 3, 2017
    Availability
    Academic Institutions and Nonprofits only
    100966-plasmid-map-sequence-id-193744

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  4. pcDNA3 H2B-mIFP T2A Mpro (C145A)

    Plasmid
    #163080
    Purpose
    Expresses inactive Mpro mutant and a near-infrared fluorescent protein mIFP fused to H2B
    Depositor
    Xiaokun Shu
    Insert
    Inactive Mpro mutant C145A and H2B-mIFP
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    163080-plasmid-map-sequence-id-320827

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  5. pDRF'- AmTryoshka1;3-LS-F138I-T78H

    Plasmid
    #100963
    Purpose
    Yeast expression of fluorescent sensor reporting the activity of ammonium transceptors. Contains a stable reference LSSmOrange nested within the reporting superfolder cpEGFP
    Depositor
    Wolf Frommer
    Article
    Ast et al Nat Commun. 2017 Sep 5;8(1):431. doi: 10
    Insert
    AmTryoshka1;3-LS-F138I-T78H (AMT1;3 Mustard Weed)
    Use
    Tags
    Expression
    Yeast
    Mutation
    F138I, T78H
    Promoter
    PMA
    Available since
    Oct. 3, 2017
    Availability
    Academic Institutions and Nonprofits only
    100963-plasmid-map-sequence-id-193742

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  6. pcDNA3 H2B-mIFP T2A Mpro (wt)

    Plasmid
    #163079
    Purpose
    Expresses wild type Mpro (active) and a near-infrared fluorescent protein mIFP fused to H2B
    Depositor
    Xiaokun Shu
    Insert
    Wild type Mpro of SARS-CoV-2 and H2B-mIFP
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    163079-plasmid-map-sequence-id-320826

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  7. pFP.E227

    Plasmid
    #138249
    Purpose
    Reporter plasmid - Expresses three fluorescent reporters from three different ECF promoters
    Depositor
    Baojun Wang
    Article
    Pinto et al Nat Commun. 2020 Mar 23;11(1):1529. doi:
    Inserts
    mCherry
    Terminator
    mTagBFP
    Terminator
    gfp
    Terminator
    Use
    Synthetic Biology
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    Promoter
    P17_up1691, P20_992, and iP16_3622
    Available since
    March 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    138249-plasmid-map-sequence-id-273529

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  8. pBALU1

    Plasmid
    #69316
    Purpose
    Encodes a recombineering cassette for tagging a target gene within a genomic clone with a fluorescent protein. Cassette: FRT-galK-FRT in the 2nd intron of gfp
    Depositor
    Oliver Hobert
    Article
    Tursun et al PLoS One. 2009;4(3):e4625. doi: 10.1371/
    Insert
    GFPint-FgF
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Sept. 18, 2015
    Availability
    Academic Institutions and Nonprofits only
    69316-plasmid-map-sequence-id-266105

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  13. MoClo Pichia toolkit

    Plasmid Kit
    #1000000108
    Purpose
    A collection of control elements and fluorescent reporters that can be used in combination with the MoClo-YTK toolkit for protein expression and secretion in yeast (Pichia pastoris).
    Depositor
    Volker Sieber
    Available since
    June 1, 2017
    Availability
    Academic Institutions and Nonprofits only

  14. pACBE-FSR (pXK-1648)

    Plasmid
    #208821
    Purpose
    Universal Fluorescent Surrogate Reporter for adenine and cytosine base editors. Key Construct: CMV-mCherry-CMV-eGFP* (ATAACG)-hU6-gRNA.
    Depositor
    Kun Xu
    Article
    Ma et al J Biol Chem. 2022 Jul;298(7):102103. doi
    Type
    Empty backbone
    Use
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, U6
    Available since
    Dec. 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    208821-plasmid-map-sequence-id-417246

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  15. pBALU9

    Plasmid
    #69324
    Purpose
    Encodes a recombineering cassette for tagging a target gene within a genomic clone with a fluorescent protein. Cassette: bicistronic; FRT-galK-FRT in 2nd GFP intron;use C-term after STOP
    Depositor
    Oliver Hobert
    Article
    Tursun et al PLoS One. 2009;4(3):e4625. doi: 10.1371/
    Insert
    gSL2-NLS-GFPint-FgF
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Sept. 10, 2015
    Availability
    Academic Institutions and Nonprofits only

  19. pRSET a sfGCaMP6s

    Plasmid
    #100022
    Purpose
    Bacterial expression of fluorescent reporter for calcium signaling, based off of GCaMP6s. cpEGFP replaced with a superfolder cpGFP variant.
    Depositor
    Wolf Frommer
    Article
    Ast et al Nat Commun. 2017 Sep 5;8(1):431. doi: 10
    Insert
    sfGCaMP6s
    Use
    Tags
    6x HIS tag and Xpress_EK tag
    Expression
    Bacterial
    Mutation
    cpEGFP replaced with superfolder cpGFP
    Promoter
    T7
    Available since
    Oct. 3, 2017
    Availability
    Academic Institutions and Nonprofits only
    100022-plasmid-map-sequence-id-194291

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Showing: 461 - 480 of 613 results