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Plasmid#210725PurposeNIR optogenetic module for conditional protein splicing of split mCherry (C-part)DepositorInsertC2
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceDec. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
mTB3-Myc tag
Plasmid#210205PurposeType 3 phage-display vector that contains kanamycin resistance and displays the Myc-tag at the N-terminus of mature protein III.DepositorInsertMyc-tag
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
PX852
Plasmid#62884PurposeC-term SpCas9 piece of inducible split-4 (Cas9(C)-FKBP split-4).DepositorInsertSpCas9 (aa536-1368)
UseCRISPRTagsFKBP12, NLS SV40, and NLS nucleoplasminExpressionMammalianMutationPromoterCBhAvailable sinceMarch 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
Vector 1174D
Plasmid#200012PurposeFluorescent based sex separator in Aedes aegypti (SEPARATOR)DepositorInsertEngineered dobulesex splicing module
UseSynthetic BiologyTagsExpressionInsectMutationEngineered dobulesex splicing modulePromoterHr5IE1Available sinceJune 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRegev_V2
Plasmid#204144PurposeSingle reporter randomly selected from splicing library for biochemical validation.DepositorInsertV2 exon in beta globin splicing minigene
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterTruncated CAGAvailable sinceAug. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRegev_V3
Plasmid#204145PurposeSingle reporter randomly selected from splicing library for biochemical validationDepositorInsertV3 exon in beta globin splicing minigene
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterTruncated CAGAvailable sinceAug. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUC19-P70a-Top-T~P70a-Bottom-T
Plasmid#87314PurposeExpresses the modular Split-Broccoli system (Top + Bottom) under the control of E. coli sigma 70 promoters.DepositorInsertSplit-Broccoli system
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterP70aAvailable sinceMay 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRegev_ExonSkip_LandingPad
Plasmid#204186PurposeBackbone for cloning individual exon sequences into beta globin minigene splicing reporter.DepositorInsertBeta globin splicing minigene
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterTruncated CAGAvailable sinceAug. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEX-6P1-OPNb-delta S
Plasmid#107984Purposebacterial expression of OPN splice variant bDepositorInsertOPNb 1038bp-17aa signal (SPP1 Human)
UseTagsGSTExpressionBacterialMutationWT splice variantPromoterAvailable sinceAug. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGEX-6P1-OPNc-delta S
Plasmid#107985Purposebacterial expression of OPN splice variant cDepositorInsertOPNc 999bp-16aa signal (SPP1 Human)
UseTagsGSTExpressionBacterialMutationWT splice variantPromoterAvailable sinceMay 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUC19-P70a-Bottom-T
Plasmid#87313PurposeExpresses the "Bottom" transcript of the Split-Broccoli system under the control of the E. coli sigma 70 promoter.DepositorInsertSplit-Broccoli system "Bottom" transcript
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterP70aAvailable sinceMay 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pUC19-Trigger-Bottom
Plasmid#87317PurposeExpresses the "Bottom" transcript of the Split-Broccoli system downstream of an RNA Toehold switch Trigger sequence.DepositorInsertSplit-Broccoli "Bottom" transcript downstream of RNA Trigger (TS2_AT01)
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterP70aAvailable sinceMay 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pUC19-P70a-Top-T
Plasmid#87312PurposeExpresses the "Top" transcript of the Split-Broccoli system under the control of the E. coli sigma 70 promoter.DepositorInsertSplit-Broccoli system "Top" transcript
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterP70aAvailable sinceJan. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
mTB3-FLAG tag
Plasmid#210204PurposeType 3 phage-display vector that contains kanamycin resistance and displays the FLAG-tag at the N-terminus of mature protein III.DepositorInsertFLAG-tag
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
mTB3-AviD tag
Plasmid#210203PurposeType 3 phage-display vector that contains kanamycin resistance and displays the AviD-tag at the N-terminus of mature protein III.DepositorInsertAviD-tag
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRegev_D1
Plasmid#204178PurposeSingle reporter with long guanine depleted (G-poor) region from splicing library.DepositorInsertExon D1 in beta globin splicing minigene
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterTruncated CAGAvailable sinceAug. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRegev_D4
Plasmid#204184PurposeSingle reporter with long guanine depleted (G-poor) region from splicing library.DepositorInsertD4 exon in beta globin splicing minigene
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterTruncated CAGAvailable sinceAug. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRegev_D2
Plasmid#204180PurposeSingle reporter with long guanine depleted (G-poor) region from splicing library.DepositorInsertExon D2 in beta globin splicing minigene
UseSynthetic BiologyTagsExpressionMammalianMutation61C>G mutationPromoterTruncated CAGAvailable sinceAug. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRegev_ExonSkip_Backbone
Plasmid#204187PurposeBackbone for cloning splicing library with truncated CAG promoter and first half of beta globin minigene reporter (exon 1 and intron 1).DepositorInsertFirst exon and intron for beta globin splicing minigene
UseTagsExpressionMammalianMutationPromoterTruncated CAGAvailable sinceAug. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
AAVS1-TRE3G-NODALvar
Plasmid#115640PurposeFor targeted integration and inducible expression of a human NODAL splice variant using doxycyclineDepositorInsertNODAL splice variant (NODAL Human)
UseTagsExpressionMammalianMutationPromoterTRE3GAvailable sinceSept. 27, 2022AvailabilityAcademic Institutions and Nonprofits only