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Showing: 461 - 480 of 2591 results

  1. pPOTv7-g418-BFP

    Plasmid
    #190259
    Purpose
    Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging
    Depositor
    Sue Vaughan
    Article
    Paterou et al bioRxiv 2023.04.21.537815
    Insert
    BFP
    Use
    For endogenous gene tagging
    Tags
    Expression
    Mutation
    Promoter
    read through
    Available since
    April 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    190259-plasmid-map-sequence-id-374841

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  5. pODN-H2BC11-TagBFP

    Plasmid
    #183867
    Purpose
    Repair template for the C-terminal tagging of H2B histones with mTagBFP in human cells using CRISPR/Cas9.
    Depositor
    Alisa Piekny
    Article
    Husser et al Open Biol. 2022 Nov;12(11):220247. doi:
    Insert
    H2BC11 homology arms with linker-mTagBFP (H2BC11 Human)
    Use
    CRISPR; Donor template
    Tags
    linker-mTagBFP
    Expression
    Mammalian
    Mutation
    Homology arms contain point mutations to remove t…
    Promoter
    Available since
    May 20, 2022
    Availability
    Academic Institutions and Nonprofits only
    183867-plasmid-map-sequence-id-362447

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  10. pAS2-his-24::tagBFP2

    Plasmid
    #124322
    Purpose
    Expressing Nuclear Localized tagBFP2
    Depositor
    Andrew Leifer
    Insert
    tagBFP2 (his-24 Nematode)
    Use
    Tags
    Expression
    Worm
    Mutation
    Promoter
    Available since
    April 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    124322-plasmid-map-sequence-id-241690

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  12. mTagBFP2-Espin-Like-C-18

    Plasmid
    #62784
    Purpose
    Localization: Actin Bundling , C1 Cloning Vector, Excitation:399, Emission:456
    Depositor
    Michael Davidson
    Insert
    EspinLike
    Use
    Tags
    mTagBFP2
    Expression
    Mammalian
    Mutation
    none
    Promoter
    CMV
    Available since
    March 10, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits

  13. gRNA-CasRx_SD1-pSV40-TagBFP

    Plasmid
    #221004
    Purpose
    Transiently express CasRx DR30 repeat with gRNA spacer. Contains SD1 mutation to enhance efficiency. SV40-TagBFP cassette to monitor transfection efficiency. Use BbsI with overhangs Fw-AAAC, Rv-AAAA.
    Depositor
    Mikko Taipale, Benjamin Blencowe
    Article
    Li et al Mol Cell. 2024 Jul 11;84(13):2573-2589.e
    Insert
    CasRx 30nt processed direct repeat with SD1 mutation
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    DR1 A>T
    Promoter
    U6
    Available since
    June 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    221004-plasmid-map-sequence-id-436033

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Showing: 461 - 480 of 2591 results