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Pooled Library#163955PurposeRLC11 pooled libraryDepositorExpressionBacterialUseCRISPRAvailable sinceOct. 1, 2021AvailabilityAcademic Institutions and Nonprofits only
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RLC12
Pooled Library#163954PurposeRLC12 pooled libraryDepositorExpressionBacterialUseCRISPRAvailable sinceOct. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
BJ5183 cells
Bacterial Strain#16398DepositorBacterial ResistanceStreptomycinSpeciesEscherichia coliAvailable sinceJune 11, 2008AvailabilityAcademic Institutions and Nonprofits only -
Murine cell surface protein knockout (McspKO) library
Pooled Library#200445PurposeA CRISPR library targeting 1,157 cell surface genes and 98 control genes with four sgRNAs per geneDepositorExpressionMammalianSpeciesMus musculusUseCRISPR, Lentiviral, and Mouse TargetingAvailable sinceOct. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSimpleII-U6-tracr-U6-crRNA(tdTomato)-NLS-NmCas9-HA-NLS(s)
Plasmid#47869PurposeAll-in-one plasmid targeting tdTomatoDepositorInsertsNmCas9
U6pr-tracrRNA
tdTomato crRNA
UseCRISPRTagsHA and NLSExpressionMammalianMutationPromoterAvailable sinceSept. 30, 2013AvailabilityAcademic Institutions and Nonprofits only -
10xHis-MBP-TEV-S. pyogenes dCas9 M1C D10A C80S H840A C574S
Plasmid#60815PurposedCas9 with single cysteine residue (M1C) for site-specific labellingDepositorInsertdCas9 M1C D10A C80S H840A C574S
UseTagsHis10 and MBPExpressionBacterialMutationM1C D10A C80S H840A C574SPromoterAvailable sinceDec. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
Gal1/10 His6 TEV Ura S. cerevisiae expression vector (12URA-B)
Plasmid#48304DepositorTypeEmpty backboneUseNaTagsHis6ExpressionMutationPromoterAvailable sinceNov. 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
Gal1/10 His6 TEV Trp S. cerevisiae expression vector (12TRP-B)
Plasmid#48301DepositorTypeEmpty backboneUseNaTagsHis6ExpressionMutationPromoterAvailable sinceNov. 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
pHIE055 ZF43x3-S mKate2 EF1a VP16-ZF43 EF1a EBFP2-P2A-BlastR
Plasmid#138736PurposeIntegration Vector, assembled with pHIE047 (TUPV1), pJM466 (TUPV2), pJM452 (TUPV3), pPD613DepositorInsertZF43x3-Spaced
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceMarch 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHIE056 ZF43x6-S mKate2 EF1a VP16-ZF43 EF1a EBFP2-P2A-BlastR
Plasmid#138737PurposeIntegration Vector, assembled with pHIE048 (TUPV1), pJM466 (TUPV2), pJM452 (TUPV3), pPD613DepositorInsertZF43x6-Spaced
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceDec. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHIE057 ZF43x12-S mKate2 EF1a VP16-ZF43 EF1a EBFP2-P2A-BlastR
Plasmid#138738PurposeIntegration Vector, assembled with pHIE049 (TUPV1), pJM466 (TUPV2), pJM452 (TUPV3), pPD613DepositorInsertZF43x12-Spaced
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceDec. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSimpleII-U6-tracr-U6-crRNA(OCT4)-NLS-NmCas9-HA-NLS(s)
Plasmid#47870PurposeAll-in-one plasmid targeting human OCT4, cuts roughly ~84bp downstream of stop codon.DepositorInsertsNmCas9
U6pr-tracrRNA
OCT4 crRNA
UseCRISPRTagsHA and NLSExpressionMammalianMutationPromoterAvailable sinceSept. 30, 2013AvailabilityAcademic Institutions and Nonprofits only -
Gal1/10 His6 TEV Ade S. cerevisiae expression vector (12ADE-B)
Plasmid#48298DepositorTypeEmpty backboneUseNaTagsHis6ExpressionMutationPromoterAvailable sinceNov. 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
Human SLC KO Library
Pooled Library#132552PurposeExpresses gRNAs for knockout of the human Solute Carrier familyDepositorExpressionMammalianSpeciesHomo sapiensUseCRISPR and LentiviralAvailable sinceMay 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
CSH130+pRCP
Bacterial Strain#195860PurposeWT E. coli strain transformed with plasmid carrying WT chromogenic protein (CP). Serves as control for suppressor strains. Grows purple when CP is induced with rhamnose.DepositorBacterial ResistanceChloramphenicolSpeciesAcropora milleporaAvailable sinceFeb. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
CSH130+pRCPam
Bacterial Strain#195859PurposeWT E. coli strain transformed with plasmid carrying chromogenic protein (CP) with amber nonsense mutation in chromophore. Serves as control for suppressor strains. No color when grown with or without rhamnose.DepositorBacterial ResistanceChloramphenicolSpeciesAcropora milleporaAvailable sinceFeb. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
PX458-3xHA-en31FnCas9ABEmax8.17d
Plasmid#201956PurposeMammalian expression plasmid of en31FnCas9ABEmax8.17d base editor with T2A-EGFP and cloning backbone for sgRNADepositorInsertbpNLS-en31FnCas9ABEmax8.17d-bpNLS-3xHA-T2A-EGFP
UseCRISPRTags3xHA, NLS, and T2A-EGFPExpressionMammalianMutationPromoterCbhAvailable sinceMay 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
PX458-3xHA-dFnCas9
Plasmid#201954PurposeMammalian expression plasmid of dead FnCas9 with T2A-EGFP and cloning backbone for sgRNADepositorInsert3xHA-NLS-dFnCas9-T2A-EGFP
UseCRISPRTags3xHA, NLS, and T2A-EGFPExpressionMammalianMutationD11A and H969A on FnCas9PromoterCbhAvailable sinceMay 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET-His6-en1dFnCas9GFP
Plasmid#201950PurposeExpression of en1dFnCas9-GFP in bacterial cellsDepositorInsertExpression of en1dFnCas9-GFP in bacterial cells
UseCRISPRTags6x His, HA, NLS, and mEGFPExpressionBacterialMutationE1369R on FnCas9PromoterT7Available sinceMay 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET-His6-en15dFnCas9GFP
Plasmid#201951PurposeExpression of en15dFnCas9-GFP in bacterial cellsDepositorInsertExpression of en15dFnCas9-GFP in bacterial cells
UseCRISPRTags6x His, HA, NLS, and mEGFPExpressionBacterialMutationE1603H on FnCas9PromoterT7Available sinceMay 7, 2024AvailabilityAcademic Institutions and Nonprofits only