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Plasmid#125171PurposeExpresses human EZH2 in insect cells, under a TEV-cleavable N-terminal polyhistidine tag.DepositorInsertEZH2 (EZH2 Human)
UseTagsHis-TEVExpressionInsectMutationPromoterPolyhedrinAvailable sinceMay 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
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pLenti-osTIR1-9xMyc-Zeo
Plasmid#225696PurposeLentiviral expression of 9xMycTag fused osTIR1and Zeocin resistance. For use with AID degron.DepositorInsertosTIR1
UseLentiviral and Synthetic BiologyTags9X MycExpressionMammalianMutationPromoterAvailable sinceDec. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
AAV-H2GH
Plasmid#182202PurposeAdeno-associated viral vector to express histone2B-HaloTag-GFP fusion, a nuclear-localized Halotag-GFP fusion constructDepositorInserthistone2B-HaloTag-GFP
UseAAVTagsExpressionMutationPromoterAvailable sinceMay 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLenti-MCS-3AID-HA-2A-mTurquoiseBSD
Plasmid#225704PurposeEmpty backbone for lentiviral expression of AID degron tagged POI and mTurquoise fused BlasticidinRDepositorTypeEmpty backboneUseLentiviral and Synthetic BiologyTagsT2A-mTurquoiseExpressionMammalianMutationPromoterpEF1AAvailable sinceOct. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti-MCS-3AID-HA-2A-mCherryBSD
Plasmid#225700PurposeEmpty backbone for lentiviral expression of AID degron tagged POI and mCherry fused BlasticidinRDepositorTypeEmpty backboneUseLentiviral and Synthetic BiologyTagsT2A-mCherryExpressionMammalianMutationPromoterpEF1AAvailable sinceOct. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti-rTetR(SE)-VP64-2A-mCherryBSD
Plasmid#225695PurposeLentiviral expression of rTetR(SE) fused to VP64 and mCherry fused BlasticidinRDepositorInsertrTetR-VP64
UseLentiviral and Synthetic BiologyTags3xFLAG and T2A-mCherryExpressionMammalianMutationPromoterAvailable sinceOct. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti-rTetR(SE)-KRAB-2A-mTurquoise
Plasmid#225690PurposeLentiviral expression of rTetR(SE) fused to ZNF10 KRAB and mTurquoise-NLSDepositorInsertrTetR-ZNF10 KRAB
UseLentiviral and Synthetic BiologyTags3xFLAG and T2A-mTurquoiseExpressionMammalianMutationPromoterAvailable sinceOct. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
Antibody#180084-rAbPurposeAnti-GFP (Aequorea victoria) recombinant mouse monoclonal antibody. Cross-reacts with Cyan and Yellow fluorescent protein (CFP and YFP, respectively).DepositorRecommended ApplicationsImmunocytochemistry, Immunohistochemistry, and Western BlotReactivitySyntheticSource SpeciesMouseIsotypeIgG2aTrial SizeAvailable to purchaseAvailable sinceMarch 29, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits
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pLenti-rTetR(SE)-EED-2A-mTurquoise
Plasmid#225692PurposeLentiviral expression of rTetR(SE) fused to EED and mTurquoise-NLSDepositorInsertrTetR-EED (EED Human, Synthetic)
UseLentiviral and Synthetic BiologyTags3xFLAG and T2A-mTurquoiseExpressionMammalianMutationPromoterAvailable sinceOct. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti-rTetR(SE)-HP1a-2A-mTurquoise
Plasmid#225691PurposeLentiviral expression of rTetR(SE) fused to HP1a and mTurquoise-NLSDepositorInsertHP1a (CBX5 Human, Synthetic)
UseLentiviral and Synthetic BiologyTags3xFLAG and T2A-mTurquoiseExpressionMammalianMutationPromoterAvailable sinceOct. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti-rTetR(SE)-DNMT3Bcd-2A-mTurquoise
Plasmid#225693PurposeLentiviral expression of rTetR(SE) fused to the catalytic domain of DNMT3B and mTurquoise-NLSDepositorInsertrTetR-DNMT3B catalytic domain (DNMT3B Human, Synthetic)
UseLentiviral and Synthetic BiologyTags3xFLAG and T2A-mTurquoiseExpressionMammalianMutationPromoterAvailable sinceOct. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti-H3.3(WT)-3AID-HA-2A-mTurquoiseBSD
Plasmid#225701PurposeLentiviral expression of AID degron tagged Wildtype H3.3 and mTurquoise fused BlasticidinRDepositorUseLentiviralTags3xAID HA and T2A-mTurquoiseExpressionMammalianMutationPromoterAvailable sinceOct. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti-H3.3(K36M)-3AID-HA-2A-mTurquoiseBSD
Plasmid#225703PurposeLentiviral expression of AID degron tagged H3.3(K36M) and mTurquoise fused BlasticidinRDepositorUseLentiviral and Synthetic BiologyTags3xAID HA and T2A-mTurquoiseExpressionMammalianMutationK36MPromoterpEF1AAvailable sinceOct. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti-H3.3(K27M)-3AID-HA-2A-mTurquoiseBSD
Plasmid#225702PurposeLentiviral expression of AID degron tagged H3.3(K27M) and mTurquoise fused BlasticidinRDepositorUseLentiviral and Synthetic BiologyTags3xAID HA and T2A-mTurquoiseExpressionMammalianMutationK27MPromoterpEF1AAvailable sinceOct. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti-H3.3(WT)-3AID-HA-2A-mCherryBSD
Plasmid#225697PurposeLentiviral expression of AID degron tagged Wildtype H3.3 and mCherry fused BlasticidinRDepositorUseLentiviral and Synthetic BiologyTags3xAID HA and T2A-mCherryExpressionMammalianMutationPromoterAvailable sinceOct. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti-H3.3(K36M)-3AID-HA-2A-mCherryBSD
Plasmid#225699PurposeLentiviral expression of AID degron tagged H3.3(K36M) and mCherry fused BlasticidinRDepositorUseLentiviral and Synthetic BiologyTags3xAID HA and T2A-mCherryExpressionMammalianMutationK36MPromoterpEF1AAvailable sinceOct. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti-H3.3(K27M)-3AID-HA-2A-mCherryBSD
Plasmid#225698PurposeLentiviral expression of AID degron tagged H3.3(K27M) and mCherry fused BlasticidinRDepositorUseLentiviral and Synthetic BiologyTags3xAID HA and T2A-mCherryExpressionMammalianMutationK27MPromoterpEF1AAvailable sinceOct. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLV-H2B-iRFP670-p2a-YFP-Rb-IRES-blasticidin
Plasmid#223962PurposeDual fluorescent reporter for histone H2B and for exogenous RbDepositorUseTagsYFP and iRFP670ExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLV-H2B-iRFP670-p2a-mCerulean-Geminin (1–110)-IRES-Neomycin
Plasmid#223959PurposeDual fluorescent reporter for histone H2B and for APC/C activityDepositorUseLentiviralTagsiRFP670 and mCeruleanExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
Ath-HEN1::T2A::mCherry::TY1::H2B-pCFJ350-ttTi5605
Plasmid#108362PurposeExpression of 2xFlag MYC tagged A.thaliana-HEN1 (Ath-HEN1, codon optimised for C. elegans) and mCherry under the promoter of choice in C. elegansDepositorInsertsUseTags2xFlag, MYC, T2A peptide, and TY1 linkerExpressionWormMutationPromoterA rab-3:Ath-HEN1:unc-54 3' UTR was cloned as…Available sinceMay 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-HDAC2-H141A
Plasmid#115346PurposeExpresses mouse HDAC2 cDNA with H141A point mutationDepositorInsertHistone deacetylase 2 (Hdac2 Mouse)
UseTagsFLAGExpressionMammalianMutationH141APromoterCMVAvailable sinceNov. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pmiRFP-H2B-C1
Plasmid#108411PurposeHistone 2B fused to monomeric near-infrared fluorescent protein miRFPDepositorInsertmiRFP-H2B (H2BC21 Human)
UseTagsmiRFPExpressionMammalianMutationPromoterCMVAvailable sinceApril 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSIN-TRE-H2BeGFP-rtTA2
Plasmid#165494PurposeDoxycycline-inducible all-in-one lentivirus vector to express H2BeGFP fusion protein. System for labelling slow-cycling cells in vivo.DepositorInsertHuman histone H2B (H2BC21 Human)
UseLentiviralTagsEGFPExpressionMutation-PromoterTetO-CMVAvailable sinceMarch 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pH2B-cherryFP
Plasmid#51001PurposeExpresses H2B-chFPDepositorInsertCherryFP
UseTagshistone2BExpressionMammalianMutationPromoterCMVAvailable sinceJune 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLenti.CAG.H2B-Dendra2.W
Plasmid#51005PurposeLentivector that expresses H2B-Dendra2 from internal CAG enhancer-promoterDepositorInsertH2B-Dendra2
UseLentiviralTagshistone2BExpressionMammalianMutationPromoterCAGAvailable sinceJune 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
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pLenti.Syn(0.5).H2B-eGFP.W
Plasmid#51004PurposeLentivector that expresses H2B-eGFP from neurons-specific synapsin promoterDepositorInsertH2B-EGFP (H2BC11 Jellyfish, Human, Synthetic)
UseLentiviralTagshistone2BExpressionMammalianMutationPromoterrat synapsinAvailable sinceJune 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLenti.PGK.H2B-chFP.W
Plasmid#51007PurposeLentivector that expresses H2B-chFP from internal human PGK promoterDepositorInsertH2B-ChFP
UseLentiviralTagshistone2BExpressionMammalianMutationPromoterhuman PGKAvailable sinceJune 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
H2B-mCherry
Plasmid#108880PurposeExpression of human histone2B fused to mCherry (nuclear marker)DepositorInsertH2B-mCherry
UseCan be used for mammalian expression or in vitro …TagsExpressionMammalianMutationPromoterAvailable sinceJune 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
Kohinoor-H2B/pcDNA3
Plasmid#67774PurposeHiston2B-targetted Kohinoor for mammalian cell expressionDepositorInsertKohinoor
UseTagsH2BExpressionMammalianMutationPromoterCMVAvailable sinceSept. 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pACAGW-H2B-PAGFP-AAV
Plasmid#33000DepositorInsertH2B-PAGFP (H2BC21 Human, Aequorea victoria)
UseAAV; Adeno associated virusTagsPAGFPExpressionMammalianMutationPromoterAvailable sinceDec. 12, 2011AvailabilityAcademic Institutions and Nonprofits only -
pH3mCHSH2
Plasmid#101053PurposeThis is a Cryptococcus neoformans mCherry expression vector with G418 drug selection marker and C. neoformans SH2 flanking sequences for genome integration.DepositorInsertCryptococcus mCherry expression plasmid
UseTagsExpressionYeastMutationPromoterCryptococcus Histone3 promoterAvailable sinceOct. 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCI Cas9 H2B-RFP
Plasmid#92397PurposeCAG-driven ubiquitous expression of Cas9 with Histone2B-RFP reporter controlled by IRESDepositorInsertCas9
UseCRISPRTagsExpressionMammalianMutationPromoterCAGAvailable sinceAug. 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCI H2B-RFP
Plasmid#92398PurposeUbiquitous expression plasmid, contains CAG promoter (CMV immediate early enhancer and chicken beta actin promoter), MCS and IRES controlled Histone2B-mRFP1 reporter.DepositorInsertIRES H2B RFP
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceAug. 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCI H2B-GFP
Plasmid#92399PurposeUbiquitous expression plasmid, contains CAG promoter (CMV immediate early enhancer and chicken beta actin promoter), MCS and IRES controlled Histone2B-EGFP reporter.DepositorInsertIRES H2B EGFP
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceAug. 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-FLEX-H2BmTagBFP2-oG
Plasmid#217976PurposeAAV transfer plasmid to express H2B-BFP and codon-optimized Rabies G proteinDepositorInsertsH2BmTagBFP2
oG
UseAAVTagsmTAGBFP2ExpressionMutationPromoterhSynAvailable sinceApril 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKCAG-H2BmCherry-OptoJNKi
Plasmid#89741PurposeExpression of light-regulated JNK inhibitor, mCherry-tagged, localised to chromatin with H2B, wild-type photosensor, inhibitor JIP11DepositorInsertOptoJNKi
UseOptogeneticsTagsHistone2B and mCherryExpressionMammalianMutationPromoterCAGAvailable sinceSept. 13, 2017AvailabilityAcademic Institutions and Nonprofits only