Addgene

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pJUMP23-1A(sfGFP)

Plasmid

#126970

Purpose

Level 1 vector. sfGFP reporter. Origin pBBR1 (medium-copy; broad-host-range)

Depositor

Chris French

Article

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

None

Promoter

Available since

July 31, 2019

Availability

Academic Institutions and Nonprofits only

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pTK24

Plasmid

#37406

Depositor

Iain Cheeseman

Article

Kiyomitsu et al Nat Cell Biol. 2012 Feb 12;14(3):311-7.

Insert

Plk1 (PLK1 Human)

Use

Tags

Mem-mCherry, S-tag, and TEV

Expression

Mammalian

Mutation

Promoter

Available since

July 19, 2012

Availability

Academic Institutions and Nonprofits only

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p15A-OptoCre-knt-P-R*

Plasmid

#189852

Purpose

Cre-inducible activation of knt for kanamycin resistance.

Depositor

Mary Dunlop

Article

Sheets et al Nat Commun. 2023 Feb 23;14(1):1034. doi:

Insert

P-loxP-TT-loxP-R*-knt

Use

Cre/Lox and Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

P (T7 phage gene 10)

Available since

Feb. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pD649-HAsp-CD274-COMP5AP-AviTag-9xHis

Plasmid

#157224

Purpose

Mammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

CD274 (CD274 Human)

Use

Tags

COMP5AP-AviTag-9xHis

Expression

Mammalian

Mutation

Promoter

CMV/SP6

Available since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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hSyn-DIO-somBiPOLES-eGFP

Plasmid

#192584

Purpose

AAV-vector for bidirectional optogenetic manipulation of Cre-positive neurons

Depositor

Simon Wiegert

Insert

somBiPOLES

Use

AAV

Tags

soma-targeting motif from Kv2.1 channel

Expression

Mammalian

Mutation

Promoter

human synapsin

Available since

Dec. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pTriEx-mCherry-Zdk1-TIAM1 DH/PH

Plasmid

#81129

Purpose

mammalian expression of Zdk1 tagged Tiam1 Dh/PH

Depositor

Klaus Hahn

Article

Wang et al Nat Methods. 2016 Jul 18. doi: 10.1038/n

Inserts

Zdk1
TIAM1 DH/PH (TIAM1 Human)

Use

Tags

mCherry

Expression

Bacterial, Insect, and Mamm…

Mutation

Promoter

Available since

Aug. 30, 2016

Availability

Academic Institutions and Nonprofits only

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pD649-HAsp-PDCD1LG2-COMP5AP-AviTag-9xHis

Plasmid

#157176

Purpose

Mammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

PDCD1LG2 (PDCD1LG2 Human)

Use

Tags

COMP5AP-AviTag-9xHis

Expression

Mammalian

Mutation

Promoter

CMV/SP6

Available since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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pD649-HAsp-PDCD1-COMP5AP-AviTag-9xHis

Plasmid

#157108

Purpose

Mammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

PDCD1 (PDCD1 Human)

Use

Tags

COMP5AP-AviTag-9xHis

Expression

Mammalian

Mutation

Promoter

CMV/SP6

Available since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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pKDM

Plasmid

#110740

Purpose

Plasmid for the insect cell expression system, two promoters, Multiplication Module M, Tn7 transposition

Depositor

Tim Richmond

Article

Fitzgerald et al Nat Methods. 2006 Dec;3(12):1021-32. doi

Type

Empty backbone

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

June 21, 2018

Availability

Academic Institutions and Nonprofits only

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Sema6a.a-AP-His

Plasmid

#72037

Purpose

Expresses the extracellular region of the Sema6A, isoform a protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Sema6a.a (Sema6a Mouse)

Use

Tags

AP-His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 18, 2016

Availability

Academic Institutions and Nonprofits only

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pDEST40-2XFL-Src-S4-UD-mCherry

Plasmid

#140306

Purpose

Expresses HA-tagged human Src-S4-UD-mCherry in mammalian cells for structure-function studies

Depositor

Mark Moasser

Article

Spassov et al Cell Rep. 2018 Oct 9;25(2):449-463.e4. d

Insert

Src deletion mutant (SRC Human)

Use

Tags

2X FL

Expression

Mammalian

Mutation

contains only the SH4 and UD regions fused to mCh…

Promoter

CMV

Available since

April 29, 2020

Availability

Academic Institutions and Nonprofits only

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Incoherent feedforward (IFF) plasmid

Plasmid

#169748

Purpose

Standalone IFF circuit plasmid that has the same implementation of the IFF subcircuit in Equalizer-L.

Depositor

Francois St-Pierre

Article

Yang et al Nat Commun. 2021 Jul 5;12(1):4132. doi:

Insert

miR(FF4) target-eGFP-miR(FF4) target-miR(FF4)

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

CMV-tetO2

Available since

June 9, 2021

Availability

Academic Institutions and Nonprofits only

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TAPBPL pmRFP-N2 (721)

Plasmid

#62000

Purpose

mammalian expression of nuclear envelope transmembrane protein

Depositor

Eric Schirmer

Article

Korfali et al Mol Cell Proteomics. 2010 Dec;9(12):2571

Insert

TAPBPL (TAPBPL Human)

Use

Tags

mRFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 28, 2015

Availability

Academic Institutions and Nonprofits only
Sema6b(L)-AP-His

Plasmid

#72039

Purpose

Expresses the extracellular region of the Sema6B protein (entire extracellular domain; ie, long), C-terminally fused to alkaline phosphatase + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Sema6b (Sema6b Mouse)

Use

Tags

AP-His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 23, 2016

Availability

Academic Institutions and Nonprofits only

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Sema6c-AP-His

Plasmid

#72041

Purpose

Expresses the extracellular region of the Sema6C protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Sema6c (Sema6c Mouse)

Use

Tags

AP-His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 24, 2016

Availability

Academic Institutions and Nonprofits only

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pMM585

Plasmid

#68884

Purpose

IPTG-inducible NanoLuc expression, pNBU2 backbone, AmpR

Depositor

Christopher Voigt, Timothy Lu

Article

Mimee et al Cell Syst. 2015 Jul 29;1(1):62-71. doi:

Inserts

NanoLuc
LacI

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 7, 2015

Availability

Academic Institutions and Nonprofits only

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pTK25

Plasmid

#37407

Depositor

Iain Cheeseman

Article

Kiyomitsu et al Nat Cell Biol. 2012 Feb 12;14(3):311-7.

Insert

Plk1 (PLK1 Human)

Use

Tags

Mem-mCherry, S-tag, and TEV

Expression

Mammalian

Mutation

K82R

Promoter

Available since

July 19, 2012

Availability

Academic Institutions and Nonprofits only

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pENTR-L3-SpCas9-HF1-2A-EGFP-L2

Plasmid

#141048

Purpose

Mutisite Gateway mediated vector construction

Depositor

Hideyuki Okano

Article

Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

Insert

SpCas9-HF1-2A-EGFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 9, 2020

Availability

Academic Institutions and Nonprofits only

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pET21a-GFP-AURKA_K162M-mCherry

Plasmid

#99881

Purpose

Expression of GFP-AURKA K162M-mCherry for protein purification in bacteria

Depositor

Marc Tramier

Article

Bertolin et al Nat Commun. 2016 Sep 14;7:12674. doi: 10

Insert

AURKA (AURKA Human)

Use

Tags

GFP and mCherry

Expression

Bacterial

Mutation

K162M (kinase-dead)

Promoter

Available since

Sept. 1, 2017

Availability

Academic Institutions and Nonprofits only

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Sema6b(S)-AP-His

Plasmid

#72040

Purpose

Expresses the extracellular region of the Sema6B protein (truncated at extracellular domain cleavage site; ie, short), C-terminally fused to alkaline phosphatase + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Sema6b (Sema6b Mouse)

Use

Tags

AP-His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 23, 2016

Availability

Academic Institutions and Nonprofits only

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mTagBFP2-CD151-7

Plasmid

#55282

Purpose

Localization: Tetraspanin/Membrane, Excitation: 399, Emission: 456

Depositor

Michael Davidson

Article

Subach et al PLoS One. 2011;6(12):e28674. Epub 2011 D

Insert

CD151 (CD151 Human)

Use

Tags

mTagBFP2

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 7, 2014

Availability

Industry, Academic Institutions, and Nonprofits
pU6_rd12_pegRNA_(PP7-C4-Q1)

Plasmid

#232435

Purpose

pegRNA with optimized 3' modifications to correct the rd12 mutation

Depositor

Gil Westmeyer

Article

Geilenkeuser et al Cell, April 2025. doi: 10.1016/j.cell.20

Insert

3'-PP7-tagged pegRNA for rd12 correction driven by human U6 promoter

Use

CRISPR

Tags

Expression

Mutation

Promoter

U6

Available since

April 10, 2025

Availability

Academic Institutions and Nonprofits only

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pU6_rd12_nsgRNA(PP7)

Plasmid

#232436

Purpose

nsgRNA for PE3b correction of the rd12 mutation, contains a PP7 in the scaffold tetraloop

Depositor

Gil Westmeyer

Article

Geilenkeuser et al Cell, April 2025. doi: 10.1016/j.cell.20

Insert

PP7-tagged nicking gRNA (PE3b) for rd12 correction driven by human U6 promoter

Use

CRISPR

Tags

Expression

Mutation

Promoter

U6

Available since

April 10, 2025

Availability

Academic Institutions and Nonprofits only

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pSLQ9714: pHR-PGK-SV40_NLS-dCasMINI-V4-NZF-c-Myc_NLS-mCherry-WPRE

Plasmid

#203220

Purpose

Expression of dCasMINI-V4-NZF-mCherry in mammalian cells

Depositor

Stanley Qi

Article

Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

Insert

dCasMINI-V4-NZF-mCherry

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

PGK

Available since

April 2, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV Ef1a DIO GeNL_SS hGtACR1

Plasmid

#233511

Purpose

Luciferase opsin fusion protein for bioluminescent optogenetics

Depositor

Ute Hochgeschwender

Article

Ikefuama et al iScience. 2025 Feb 13;28(3):112022. doi:

Inserts

mNeonGreen
ISN-IE
hGtACR1

Use

AAV

Tags

Gaussia luciferase signal sequence

Expression

Mutation

Promoter

Available since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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Escherichia coli DH10B-MOB

Bacterial Strain

#229395

Purpose

Escherichia coli DH10B DAP-auxotrophic strain containing an integrated RK2/RP4 mobilization vector to conjugate plasmids with an IncP origin of transfer (modified pTA-Mob 2.0, AddGene Plasmid #149662)

Depositor

Michael Springer

Article

Soltysiak et al ACS Synthetic Biology. 2025. doi: 10.102

Bacterial Resistance

60 ug/ml of dap

Species

Escherichia coli

Available since

March 24, 2025

Availability

Academic Institutions and Nonprofits only
pEYN12-mflon

Plasmid

#165581

Purpose

Lon protease from Mesoplasma florum

Depositor

Daniel Ducat

Article

Sakkos et al ACS Synth Biol. 2021 Jul 16;10(7):1667-1

Insert

lon protease

Use

Tags

Expression

Bacterial

Mutation

Promoter

Ptrc

Available since

March 5, 2025

Availability

Academic Institutions and Nonprofits only

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pdCas9-tdTomato-VPR

Plasmid

#232632

Purpose

VPR activation domain coupled to catalytically inactive dCas9 for localization; contains tdTomato for labeling and as a spacer

Depositor

Karsten Rippe

Insert

dCas9-tdTomato-VPR

Use

CRISPR

Tags

NLS

Expression

Mammalian

Mutation

none

Promoter

CMV

Available since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pCHW111101

Plasmid

#222452

Purpose

Level 1, Position 7 containing CBS4-miniDFR::Cre::NOS.

Depositor

Chih-Hang Wu

Article

Chiang et al bioRxiv 2024.03.23.586378

Insert

CBS4-miniDFR::Cre::NOS

Use

Synthetic Biology

Tags

Expression

Plant

Mutation

no

Promoter

Available since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pUdOm2.4

Plasmid

#210281

Purpose

Empty backbone with ampicillin resistance for low expression in mammalian cells

Depositor

Corrie daCosta

Article

Manigat et al ACS Synth Biol. 2024 Jan 18. doi: 10.102

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 10, 2024

Availability

Academic Institutions and Nonprofits only

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