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Plasmid#133525PurposesfGFP driven by a T7 promoter with the tetO sequence 6 bp downstream from the promoter start siteDepositorInsertsfGFP
UseTagsStrep tag and TEV siteExpressionBacterialMutationWTPromoterT7Available sinceDec. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pY71-tet.I.2
Plasmid#133524PurposesfGFP driven by a T7 promoter with the tetO sequence 5 bp downstream from the promoter start siteDepositorInsertsfGFP
UseTagsStrep tag and TEV siteExpressionBacterialMutationWTPromoterT7Available sinceDec. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pY71-tet.I.4
Plasmid#133526PurposesfGFP driven by a T7 promoter with the tetO sequence 7 bp downstream from the promoter start siteDepositorInsertsfGFP
UseTagsStrep tag and TEV siteExpressionBacterialMutationWTPromoterT7Available sinceDec. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pY71-tet.I.1
Plasmid#133523PurposesfGFP driven by a T7 promoter with the tetO sequence 4 bp downstream from the promoter start siteDepositorInsertsfGFP
UseTagsStrep tag and TEV siteExpressionBacterialMutationWTPromoterT7Available sinceDec. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJSC176 - Bacterial expression plasmid for SpCas9 + Q926A variant
Plasmid#101206PurposeBacterial expression plasmid for SpCas9 + Q926A variantDepositorInsertSpCas9 variant Q926A
UseTags10x His, MBP, and TEV siteExpressionBacterialMutationQ926APromoterT7Available sinceNov. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET151-Vps4(101-437)-Hcp1 K321D
Plasmid#89288PurposeBacterial expression of S. cerevisiae Vps4 AAA ATPase cassette fused to P. aeruginosa Hcp1DepositorInsertVacuolar protein sorting-associated protein 4 (VPS4 Budding Yeast)
UseTags6xHis-V5-TEV and Hcp1ExpressionBacterialMutationdeleted amino acids 1-100, mutated Lys-321 to AspPromoterT7Available sinceApril 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET151-Vps4(101-437)-Hcp1 R325D
Plasmid#89289PurposeBacterial expression of S. cerevisiae Vps4 AAA ATPase cassette fused to P. aeruginosa Hcp1DepositorInsertVacuolar protein sorting-associated protein 4 (VPS4 Budding Yeast)
UseTags6xHis-V5-TEV and Hcp1ExpressionBacterialMutationdeleted amino acids 1-100, mutated Arg-325 to AspPromoterT7Available sinceApril 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET151-Vps4(101-437)-Hcp1 E322A
Plasmid#87748PurposeBacterial expression of S. cerevisiae Vps4 AAA ATPase cassette fused to P. aeruginosa Hcp1DepositorInsertVacuolar protein sorting-associated protein 4 (VPS4 Budding Yeast)
UseTags6xHis-V5-TEV and Hcp1ExpressionBacterialMutationdeleted amino acids 1-100, mutated Glu-322 to AlaPromoterT7Available sinceApril 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET151-Vps4(101-437)-Hcp1 R325A
Plasmid#87749PurposeBacterial expression of S. cerevisiae Vps4 AAA ATPase cassette fused to P. aeruginosa Hcp1DepositorInsertVacuolar protein sorting-associated protein 4 (VPS4 Budding Yeast)
UseTags6xHis-V5-TEV and Hcp1ExpressionBacterialMutationdeleted amino acids 1-100, mutated Arg-325 to AlaPromoterT7Available sinceApril 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET151-Vps4(101-437)-Hcp1 K321A
Plasmid#87747PurposeBacterial expression of S. cerevisiae Vps4 AAA ATPase cassette fused to P. aeruginosa Hcp1DepositorInsertVacuolar protein sorting-associated protein 4 (VPS4 Budding Yeast)
UseTags6xHis-V5-TEV and Hcp1ExpressionBacterialMutationdeleted amino acids 1-100, mutated Lys-321 to AlaPromoterT7Available sinceApril 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET151-hUCH37 (ISF1)(M148D/F149D)
Plasmid#71388PurposeExpression of hUCH37 (M148D/F149D) in E.coliDepositorInserthUCH37 (ISF1) M148D/F149D (UCHL5 Human)
UseTagsHIS-TEV sequenceExpressionBacterialMutationchanged methionine 148 to aspartate, phenylalanin…PromoterT7Available sinceDec. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
spCas9-NLS-MAV
Plasmid#124212PurposeSpCas9-NLS 17 amino acid linker to monoavidin (SpCas9-NLS-MAV)DepositorInsertcas9
UseCRISPRTagsHis6, MBP, TEV Cleavage site, and two copies of S…ExpressionBacterialMutationPromoterT7Available sinceJune 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFastBac Dual α1B/β2B Tubulin
Plasmid#170553PurposeEncodes codon-optimized human α1B and β2B tubulin for expression in and purification from insect cells.DepositorUseTagsAP linker, GS linker (GGSGG), His10 tag, L21 enha…ExpressionInsectMutationPromoterAvailable sinceJune 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET41-MBP-t-A3A-t-His
Plasmid#109231PurposeBacterial expression of A3A with N-terminal MBP an C-terminal His tag, both cleavable with TEV proteaseDepositorInserthuman APOBEC3A (APOBEC3A Optimized for E. coli expression, Human)
UseTagsHis and MBPExpressionBacterialMutationPromoterAvailable sinceOct. 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJSC173 - Bacterial expression plasmid for SpCas9 Cluster 1 (HypaCas9) variant
Plasmid#101218PurposeBacterial expression plasmid for SpCas9 Cluster 1 (HypaCas9) variantDepositorInsertSpCas9 variant N692A/M694A/Q695A/H698A
UseTags10x His, MBP, and TEV siteExpressionBacterialMutationN692A, M694A, Q695A and H698APromoterT7Available sinceNov. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
(SUMO)10-(SIM)5
Plasmid#126866PurposeBacterial expression plasmid for multivalent scaffold of (SUMO)10-(SIM)5 biomolecular condensates to recruit SIM-containing client proteins.DepositorInsert10 repeats of human SUMO3 and 5 repeats of human SUMO interactive motif (SIM) from PIASx each separated by (GGS)4 (SUMO3 Human)
UseTagsExpressionBacterialMutationPromotertacAvailable sinceNov. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFastBac Dual α1B/β3 Tubulin
Plasmid#170552PurposeEncodes codon-optimized human α1B and β3 tubulin for expression in and purification from insect cells.DepositorUseTagsAP linker, GS linker (GGSGG), His10 tag, L21 enha…ExpressionInsectMutationPromoterAvailable sinceJune 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAD16_CAD_12-490_pvp008
Plasmid#124842PurposeExpresses Aspergillus terreus CAD in E.coliDepositorInsertcis-aconitate decarboxylase
UseTagsStrepTag and TEV siteExpressionBacterialMutationdeleted amino acids 1-11, synthetic codon optimiz…PromoterT7Available sinceSept. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGEX-HCF-1rep1wt
Plasmid#84606PurposePlasmid contains HCF-1rep1wt with GST-tagDepositorInsertHCF-1rep1wt (HCFC1 Human)
UseTagsGST-TEV and HisExpressionBacterialMutationPromoterT7Available sinceSept. 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHY002_Strep_Avi_hsDCAF15
Plasmid#174479PurposeExpresses DCAF15 in insect cellsDepositorInsertDCAF 15 (DCAF15 Human)
UseTagsStrepII-Avi-TEVExpressionInsectMutationPromoterAvailable sinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only