Addgene

We narrowed to 8,628 results for: CAG

Showing: 4761 - 4780 of 8628 results

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PIK3CG gRNA (BRDN0001147429)

Plasmid

#76333

Purpose

3rd generation lentiviral gRNA plasmid targeting human PIK3CG

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PIK3CG (PIK3CG Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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SCYL1 gRNA (BRDN0001146995)

Plasmid

#76065

Purpose

3rd generation lentiviral gRNA plasmid targeting human SCYL1

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

SCYL1 (SCYL1 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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ROS1 gRNA (BRDN0001147358)

Plasmid

#75993

Purpose

3rd generation lentiviral gRNA plasmid targeting human ROS1

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

ROS1 (ROS1 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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NTRK1 gRNA (BRDN0001148112)

Plasmid

#75965

Purpose

3rd generation lentiviral gRNA plasmid targeting human NTRK1

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

NTRK1 (NTRK1 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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TEK gRNA (BRDN0001149392)

Plasmid

#75725

Purpose

3rd generation lentiviral gRNA plasmid targeting human TEK

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

TEK (TEK Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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WNK2 gRNA (BRDN0001148439)

Plasmid

#75670

Purpose

3rd generation lentiviral gRNA plasmid targeting human WNK2

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

WNK2 (WNK2 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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WNK2 gRNA (BRDN0001162416)

Plasmid

#75671

Purpose

3rd generation lentiviral gRNA plasmid targeting human WNK2

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

WNK2 (WNK2 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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NEK8 gRNA (BRDN0001148289)

Plasmid

#76942

Purpose

3rd generation lentiviral gRNA plasmid targeting human NEK8

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

NEK8 (NEK8 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

June 28, 2016

Availability

Academic Institutions and Nonprofits only

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tet-pLKO.1_puro_shJUND #2

Plasmid

#136583

Purpose

Expresses an inducible short hairpin targeting human JUND sequence

Depositor

Kevin Janes

Article

Pereira et al Sci Signal. 2020 Apr 14;13(627). pii: 13

Insert

shJUNDv2 (JUND Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Availability

Academic Institutions and Nonprofits only

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elavl3:hGCGR-TEVcs-QF;he1.1:CFP

Plasmid

#218852

Purpose

D. rerio elavl3 promoter driving expression of human glucagon receptor, TEV protease recognition site, QF fusion protein for use in trans-Tango

Depositor

Marnie Halpern

Article

Coomer et al Nat Neurosci. 2025 Jan;28(1):189-200. do

Insert

hGCGR (GCGR Human)

Use

Tags

QF fusion protein

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pLKO.1-shCELF4-4405

Plasmid

#115466

Purpose

Constitutive lentiviral expression

Depositor

William Hahn

Article

Li et al Elife. 2018 Jul 30;7. pii: 37184. doi: 1

Insert

shCELF4-4405 (CELF4 Human)

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 12, 2018

Availability

Academic Institutions and Nonprofits only

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TFORF0889

Plasmid

#143735

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

ZNF384 (ZNF384 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

April 17, 2024

Availability

Academic Institutions and Nonprofits only

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LMPd Ametrine Got2 shRNA#3

Plasmid

#220593

Purpose

Retroviral vector with Ametrine marker for expression shRNA with an "UltramiR" microRNA scaffold

Depositor

Russell Jones

Article

Ma et al bioRxiv [Preprint]. 2023 Jun 11:2023.06.

Insert

Got2 shRNA (Got2 Mouse)

Use

Retroviral

Tags

Expression

Mutation

Promoter

Available since

June 17, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-PDGFR-TevCs-43LK-iLID(139)-NES-NES-TevN-iLID-TEVseq-tTA

Plasmid

#210501

Purpose

expresses LAUNCHER component in mammalian cells

Depositor

Dongmin Lee

Article

Cui et al Nat Chem Biol. 2024 Mar;20(3):353-364. d

Insert

PDGFR-TevCs-43LK-iLID(139)-NES-NES-TevN-iLID-TEVseq-tTA

Use

Tags

Myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pC0043-PspCas13b-gRNA LINE1

Plasmid

#223699

Purpose

gRNA of dPspCas13b-FTO targeting LINE1

Depositor

Chuan He

Article

Wei et al Science. 2022 May 27;376(6596):968-973.

Insert

gRNA target sequence for LINE1

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 20, 2024

Availability

Academic Institutions and Nonprofits only

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AAV2 for Split-PE, gRNAs + MMLV-RT(dRH)-P2A-eGFP (PKC1002)

Plasmid

#190112

Purpose

AAV2 for expression of pegRNA and ngRNA (HEKs3, CTT insertion) + MMLV-RT(dRH)-P2A-eGFP

Depositor

Keith Joung

Article

Grunewald et al Nat Biotechnol. 2023 Mar;41(3):337-343.

Inserts

MMLV-RT(dRH)
U6-pegRNA(HEKs3, CTT ins)-H1-ngRNA(HEK s3)

Use

AAV

Tags

bpNLS and bpNLS-P2A-eGFP

Expression

Mutation

mutations from RT in PE2 and truncation of RNAse …

Promoter

EFS and U6, H1

Available since

Sept. 26, 2022

Availability

Academic Institutions and Nonprofits only

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FCM101

Plasmid

#194298

Purpose

E. coli expression vector for monobody adaptor, FCM101, that bind to the Fc region of mouse IgG1 subclass. It contains a single Cys residue at the C-terminus for chemical conjugation, N-terminal His6

Depositor

Shohei Koide

Article

Albert et al Nat Commun. 2022 Oct 11;13(1):5998. doi:

Insert

FCM101

Use

Tags

His tag, FLAG tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

March 31, 2023

Availability

Academic Institutions and Nonprofits only

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pCDNA3 IRF7(5’UTR)-FF

Plasmid

#85490

Purpose

Firefly luciferase under the control of IRF7 5'UTR

Depositor

Ivan Topisirovic

Article

Gandin et al Genome Res. 2016 May;26(5):636-48. doi:

Insert

5'UTR of IRF7 (Irf7 Mouse)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 4, 2017

Availability

Academic Institutions and Nonprofits only

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pLentiCrisprV2 sgRNA hPER2c-term #3

Plasmid

#179456

Purpose

Lentiviral Crispr/Cas9 plasmid targeting hPER2 at C-terminus to generate C-terminal knock-in

Depositor

Achim Kramer

Article

Gabriel et al Nat Commun. 2021 Jun 18;12(1):3796. doi:

Insert

sgRNA targeting human PER2

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

hU6

Available since

March 29, 2022

Availability

Academic Institutions and Nonprofits only

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SITS-MmCav1-EGFP-APEX2

Plasmid

#170525

Purpose

Vector expression of MmCav1-EGFP-APEX2 in Leishmania cell free system. Parton lab clone JEJ

Depositor

Rob Parton

Article

Rae et al Elife. 2021 Apr 27;10. pii: 64630. doi:

Insert

Caveolin1 (Cav1 Mouse)

Use

Synthetic Biology

Tags

APEX2

Expression

Mutation

Promoter

Available since

Sept. 13, 2021

Availability

Academic Institutions and Nonprofits only

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