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Plasmid#200203PurposeExpression of truncated HSF1 (DBD and HR)DepositorInsertH1DBD-Halo-HA (Hsf1 Mouse)
UseTagsHalo; HAExpressionMammalianMutationHSF1 (1-399)PromoterEF_1alphaAvailable sinceMay 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
H1DBD-HR-Halo
Plasmid#200204PurposeExpression of truncated HSF1 (DBD and HR)DepositorInsertH1DBD-HR-Halo (Hsf1 Mouse)
UseTagsHaloExpressionMammalianMutationHSF1 (1-672)PromoterEF_1alphaAvailable sinceMay 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
mTln1-F2F3-P229L-pET151
Plasmid#177879PurposeExpresses mouse Talin 1 F2F3-P229L domains in bacterial cellsDepositorInsertmouse Talin 1 F2F3 P229L mutant (Tln1 Mouse)
UseTagsHis-tag, TEV cleavage siteExpressionBacterialMutationchanged Proline 229 to Leucine (P229L)PromoterT7Available sinceSept. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
mTln1-F2-P229S-pET151
Plasmid#177871PurposeExpresses mouse Talin 1 F2-P229S domain in bacterial cellsDepositorInsertmouse Talin 1 F2 P229S mutant (Tln1 Mouse)
UseTagsHis-tag, TEV cleavage siteExpressionBacterialMutationchanged Proline 229 to Serine (P229S)PromoterT7Available sinceSept. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
mTln1-F2-P229L-pET151
Plasmid#177876PurposeExpresses mouse Talin 1 F2-P229L domain in bacterial cellsDepositorInsertmouse Talin 1 F2 P229L mutant (Tln1 Mouse)
UseTagsHis-tag, TEV cleavage siteExpressionBacterialMutationchanged Proline 229 to Leucine (P229L)PromoterT7Available sinceSept. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
mTln1-F2F3-P229S-pET151
Plasmid#177878PurposeExpresses mouse Talin 1 F2F3-P229S domains in bacterial cellsDepositorInsertmouse Talin 1 F2F3 P229S mutant (Tln1 Mouse)
UseTagsHis-tag, TEV cleavage siteExpressionBacterialMutationchanged Proline 229 to Serine (P229S)PromoterT7Available sinceSept. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTF101.1gw3
Plasmid#172181PurposeA binary plasmid for gateway 3-Entry in pTF101.1 background (attR1 & attR2) containing double CaMV 35S promoter and TEV enhancer to drive bialophos resistance gene (bar) for plant transformation.DepositorInsertattR3-CmR-ccdB-attR4
UseTagsExpressionPlantMutationPromoterlacUV5 promoterAvailable sinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHIs8-4b-SaDAH::G226D
Plasmid#140130PurposeExpresses SaDAH::G226D variant with N-terminal 8xHis-tag in BL21 E. coliDepositorInsertDechloroacutumine halogenase G226D variant from Sinomenium acutum
UseTags8x-His, TEV protease site (ENLYFQ)ExpressionBacterialMutationchanged Gly226 to Asp226PromoterT7Available sinceApril 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pY71-tet.I.-1
Plasmid#133539PurposesfGFP driven by a T7 promoter with the tetO sequence 36 bp upstream from the promoter start siteDepositorInsertsfGFP
UseTagsStrep tag and TEV siteExpressionBacterialMutationWTPromoterT7Available sinceDec. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pY71-tet.I.25
Plasmid#133538PurposesfGFP driven by a T7 promoter with the tetO sequence 28 bp downstream from the promoter start siteDepositorInsertsfGFP
UseTagsStrep tag and TEV siteExpressionBacterialMutationWTPromoterT7Available sinceDec. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pY71-tet.I.20
Plasmid#133537PurposesfGFP driven by a T7 promoter with the tetO sequence 23 bp downstream from the promoter start siteDepositorInsertsfGFP
UseTagsStrep tag and TEV siteExpressionBacterialMutationWTPromoterT7Available sinceDec. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pY71-tet.I.14
Plasmid#133535PurposesfGFP driven by a T7 promoter with the tetO sequence 17 bp downstream from the promoter start siteDepositorInsertsfGFP
UseTagsStrep tag and TEV siteExpressionBacterialMutationWTPromoterT7Available sinceDec. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pY71-tet.I.13
Plasmid#133534PurposesfGFP driven by a T7 promoter with the tetO sequence 16 bp downstream from the promoter start siteDepositorInsertsfGFP
UseTagsStrep tag and TEV siteExpressionBacterialMutationWTPromoterT7Available sinceDec. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pY71-tet.I.12
Plasmid#133533PurposesfGFP driven by a T7 promoter with the tetO sequence 15 bp downstream from the promoter start siteDepositorInsertsfGFP
UseTagsStrep tag and TEV siteExpressionBacterialMutationWTPromoterT7Available sinceDec. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pY71-tet.I.11
Plasmid#133532PurposesfGFP driven by a T7 promoter with the tetO sequence 14 bp downstream from the promoter start siteDepositorInsertsfGFP
UseTagsStrep tag and TEV siteExpressionBacterialMutationWTPromoterT7Available sinceDec. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pY71-tet.I.10
Plasmid#133531PurposesfGFP driven by a T7 promoter with the tetO sequence 13 bp downstream from the promoter start siteDepositorInsertsfGFP
UseTagsStrep tag and TEV siteExpressionBacterialMutationWTPromoterT7Available sinceDec. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pY71-tet.I.9
Plasmid#133530PurposesfGFP driven by a T7 promoter with the tetO sequence 12 bp downstream from the promoter start siteDepositorInsertsfGFP
UseTagsStrep tag and TEV siteExpressionBacterialMutationWTPromoterT7Available sinceDec. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pY71-tet.I.7
Plasmid#133529PurposesfGFP driven by a T7 promoter with the tetO sequence 10 bp downstream from the promoter start siteDepositorInsertsfGFP
UseTagsStrep tag and TEV siteExpressionBacterialMutationWTPromoterT7Available sinceDec. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pY71-tet.I.6
Plasmid#133528PurposesfGFP driven by a T7 promoter with the tetO sequence 9 bp downstream from the promoter start siteDepositorInsertsfGFP
UseTagsStrep tag and TEV siteExpressionBacterialMutationWTPromoterT7Available sinceDec. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pY71-tet.I.5
Plasmid#133527PurposesfGFP driven by a T7 promoter with the tetO sequence 8 bp downstream from the promoter start siteDepositorInsertsfGFP
UseTagsStrep tag and TEV siteExpressionBacterialMutationWTPromoterT7Available sinceDec. 20, 2019AvailabilityAcademic Institutions and Nonprofits only