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Showing: 4741 - 4760 of 25118 results

  3. pVITRO-Fab_MTX-LUCID

    Plasmid
    #119047
    Purpose
    Plasmid encoding the mammalian expression of the Metotraxate LUCID based on antibodies (BRET semi-synthetic biosensor)
    Depositor
    Kai Johnsson
    Article
    Xue et al Angew Chem Int Ed Engl. 2017 Jun 12;56(2
    Inserts
    Fab_MTX-LUCID_part1
    Fab_MTX-LUCID_part2
    Use
    Tags
    FLAG-tag, His-tag, and Strep-tag
    Expression
    Mammalian
    Mutation
    Promoter
    EF-1α + CMV/SV40 enhancers and IRES
    Availability
    Academic Institutions and Nonprofits only
    119047-plasmid-map-sequence-id-231808

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  4. pVITRO-Fab_Theo-LUCID

    Plasmid
    #119048
    Purpose
    Plasmid encoding the mammalian expression of the Theophiline LUCID based on antibodies (BRET semi-synthetic biosensor)
    Depositor
    Kai Johnsson
    Article
    Xue et al Angew Chem Int Ed Engl. 2017 Jun 12;56(2
    Inserts
    Fab_Theo-LUCID_part1
    Fab_Theo-LUCID_part2
    Use
    Tags
    FLAG-tag, His-tag, and Strep-tag
    Expression
    Mammalian
    Mutation
    Promoter
    EF-1α + CMV/SV40 enhancers and IRES
    Availability
    Academic Institutions and Nonprofits only
    119048-plasmid-map-sequence-id-231811

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  5. pVITRO-Fab_Quin-LUCID

    Plasmid
    #119049
    Purpose
    Plasmid encoding the mammalian expression of the Quinine LUCID based on antibodies (BRET semi-synthetic biosensor)
    Depositor
    Kai Johnsson
    Article
    Xue et al Angew Chem Int Ed Engl. 2017 Jun 12;56(2
    Inserts
    Fab_Quin-LUCID_part1
    Fab_Quin-LUCID_part2
    Use
    Tags
    FLAG-tag, His-tag, and Strep-tag
    Expression
    Mammalian
    Mutation
    Promoter
    EF-1α + CMV/SV40 enhancers and IRES
    Availability
    Academic Institutions and Nonprofits only
    119049-plasmid-map-sequence-id-231810

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  7. pTHRB-donor

    Plasmid
    #112333
    Purpose
    CRISPR donor plasmid to tag human transcription factor THRB with GFP
    Depositor
    Kevin White
    Insert
    THRB homology arms flanking EGFP-IRES-Neo cassette (THRB Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    112333-plasmid-map-sequence-id-228563

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  9. pcDNA3.1 Flag YFP hNKCC1 extracellular-cysteineless (NT864)

    Plasmid
    #49069
    Purpose
    Expresses human NKCC1 mutant lacking extracellular cysteine residues and with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.
    Depositor
    Biff Forbush
    Article
    Somasekharan et al PLoS One. 2013 Dec 5;8(12):e82060. doi:
    Insert
    human NKCC1 (synthetic) (SLC12A2 Synthetic, Human)
    Use
    Tags
    3x Flag and mVenus
    Expression
    Mammalian
    Mutation
    C563S,C568S,C577S,C582S in hNKCC1 in NT17
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
    49069-plasmid-map-sequence-id-72196

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  10. pcDNA3.1 Flag YFP hNKCC1 ECL4 deletion (NT865)

    Plasmid
    #49070
    Purpose
    Expresses human NKCC1 truncation mutant lacking extracellular loop #4 (aa562-582) and with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.
    Depositor
    Biff Forbush
    Article
    Somasekharan et al PLoS One. 2013 Dec 5;8(12):e82060. doi:
    Insert
    human NKCC1 (synthetic) (SLC12A2 Synthetic, Human)
    Use
    Tags
    3x Flag and mVenus
    Expression
    Mammalian
    Mutation
    ECL4 deletion of amino acids 562-582 in hNKCC1 in…
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
    49070-plasmid-map-sequence-id-72197

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  11. pcDNA3.1 Flag YFP hNKCC1 TM-cysteineless (NT859)

    Plasmid
    #49078
    Purpose
    Expresses human NKCC1 mutant lacking cysteine residues within transmembrane domains and with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic,containing convenient restriction sites
    Depositor
    Biff Forbush
    Article
    Somasekharan et al PLoS One. 2013 Dec 5;8(12):e82060. doi:
    Insert
    human NKCC1 (synthetic) (SLC12A2 Synthetic, Human)
    Use
    Tags
    3x Flag and mVenus
    Expression
    Mammalian
    Mutation
    C295A, C543M, C630S, C723S, C724V in hNKCC1 in NT…
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
    49078-plasmid-map-sequence-id-72207

    Enlarge

  12. pcDNA3.1 Flag YFP hNKCC1 cysteineless (NT868)

    Plasmid
    #49079
    Purpose
    Expresses human NKCC1 mutant lacking cysteine residues and with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.
    Depositor
    Biff Forbush
    Article
    Somasekharan et al PLoS One. 2013 Dec 5;8(12):e82060. doi:
    Insert
    human NKCC1 (synthetic) (SLC12A2 Synthetic, Human)
    Use
    Tags
    3x Flag and mVenus
    Expression
    Mammalian
    Mutation
    C295A, C543M, C563S,C568S,C577S,C582S, C630S, C72…
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
    49079-plasmid-map-sequence-id-72208

    Enlarge

  13. pcDNA3.1 Flag YFP hNKCC1 intracellular-cysteineless (NT855)

    Plasmid
    #49080
    Purpose
    Expresses human NKCC1 mutant lacking cysteine residues within intracellular loops and with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.
    Depositor
    Biff Forbush
    Article
    Somasekharan et al PLoS One. 2013 Dec 5;8(12):e82060. doi:
    Insert
    human NKCC1 (synthetic) (SLC12A2 Synthetic, Human)
    Use
    Tags
    3x Flag and mVenus
    Expression
    Mammalian
    Mutation
    C791S, C820S, C1052S in hNKCC1 in NT17
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
    49080-plasmid-map-sequence-id-72210

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  14. pGS1034

    Plasmid
    #63796
    Purpose
    Vector to create a cassette for homologous recombination in S. cerevisiae; adds a C-terminal CBP-3xFLAG-TEV-ProtA triple tag and a LEU2 selection marker
    Depositor
    Monika Golas, Bjoern Sander
    Article
    Lin et al Biochim Biophys Acta. 2015 Mar;1854(3):1
    Insert
    CBP-3xFLAG-TEV-Protein A followed by KlLEU2
    Use
    E. coli cloning vector
    Tags
    CBP-3xFLAG-TEV-Protein A
    Expression
    Mutation
    Promoter
    none
    Availability
    Academic Institutions and Nonprofits only
    63796-plasmid-map-sequence-id-110311

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  17. pSMPUW_6.5_M2_Ef1a-V5-gateway

    Plasmid
    #128441
    Purpose
    lentiviral destination vector with Ef1a promoter and N-terminal V5 tag
    Depositor
    Lindy Barrett
    Insert
    Ef1a promoter and N-terminal V5 tag
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    128441-plasmid-map-sequence-id-256457

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  18. pSMPUW_7.1_M2_hcSyn-gateway-V5

    Plasmid
    #128457
    Purpose
    lentiviral destination vector with hcSyn promoter and C-terminal V5 tag
    Depositor
    Lindy Barrett
    Insert
    hcSyn promoter and C-terminal V5 tag
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    128457-plasmid-map-sequence-id-270524

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  19. Yes-EGFP

    Plasmid
    #110497
    Purpose
    Fluorescent Yes
    Depositor
    Bernardo Mainou
    Insert
    Yes (YES1 Human)
    Use
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    110497-plasmid-map-sequence-id-210495

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  20. EGFP-Rab13-Q67L

    Plasmid
    #110502
    Purpose
    Constitutive Active
    Depositor
    Bernardo Mainou
    Insert
    Rab13-Q67L (RAB13 Human)
    Use
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Q67L
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    110502-plasmid-map-sequence-id-210501

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Showing: 4741 - 4760 of 25118 results