Addgene

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pmCherry-CT-Tagging

Plasmid

#165869

Purpose

Template for amplification of mcherry C-terminal tagging cassette

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

Vector - CT Tagging

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pEGFP-NT-Tagging

Plasmid

#165868

Purpose

Template for amplification of EGFP N-terminal tagging cassette

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

Vector - NT Tagging

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pFC31 attB O1

Plasmid

#165843

Purpose

_C31 integrase attB site in pColE1_Omega1

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

_C31 attB

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pFC31 attB O2

Plasmid

#165844

Purpose

_C31 integrase attB site in pColE1_Omega2

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

_C31 attB

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pSV40L-TN

Plasmid

#165827

Purpose

Viral Simian virus 40 Late poly(a) terminator element Compatible with transposon 3' ITRs

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

SV40L

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pmCherry TL5

Plasmid

#165833

Purpose

mCherry red fluorescent protein for C-terminal tagging

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

mCherry

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pEGFP TL1

Plasmid

#165834

Purpose

sfGFP green fluorescent protein for N-terminal tagging

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

EGFP

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pEGFP TL5

Plasmid

#165835

Purpose

sfGFP green fluorescent protein for C-terminal tagging

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

EGFP

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pGGSx4 CT

Plasmid

#165811

Purpose

Flexible peptide linker of 4 repeats of GGS, for assembly of C-terminal TL5 tags to TL4 parts

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

GGSx4 CT

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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p4xLexAOp-A1

Plasmid

#165814

Purpose

Alpha1 4xLexAOp for assembly of larger LexAOp response elements

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

4xLexAOp-A1

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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p4xLexAOp-A2

Plasmid

#165815

Purpose

Alpha2 4xLexAOp for assembly of larger LexAOp response elements

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

4xLexAOp-A2

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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p4xLexAOp-O2

Plasmid

#165816

Purpose

Omega2 4xLexAOp for assembly of larger LexAOp response elements

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

4xLexAOp-O2

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
p4xLexAOp-O1

Plasmid

#165817

Purpose

Omega1 4xLexAOp for assembly of larger LexAOp response elements

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

4xLexAOp-O1

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pLexA BD

Plasmid

#165821

Purpose

LexA DNA binding domain, requires assembly with TL2-TL4 linker and TL5 activation domain

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

LexA BD

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pQFBD

Plasmid

#165823

Purpose

QF DNA binding domain, requires assembly with TL2-TL4 linker and TL5 activation domain

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

QFBD

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pQFAD

Plasmid

#165824

Purpose

QF activation domain, requires assembly with TL2-TL4 linker and TL1 DNA binding domain

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

QFAD

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pGGGSx4

Plasmid

#165807

Purpose

Flexible peptide linker of 4 repeats of GGGS, for assembly of TL1 to TL5 parts

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

GGGSx4

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pT2A

Plasmid

#165809

Purpose

Viral T2A linker element for assembly of TL3 to TL5 parts

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

T2A

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pGGSx4 NT

Plasmid

#165810

Purpose

Flexible peptide linker of 4 repeats of GGS, for assembly of N-terminal TL1 tags to TL3 parts

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

GGSx4 NT

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pDmVasa

Plasmid

#165783

Purpose

Drosohphila vasa germline promoter and 5'UTR

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

Vasa promoter

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge