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Plasmid#174448PurposeExpress GFP-tagged RNase H1 D210N in E. coli.DepositorInsertRNase H1 (RNASEH1 Human)
UseTagsHis-tag, GFPExpressionBacterialMutationD210NPromoterT7 promoterAvailable sinceSept. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLV hUbC-dSaCas9-KRAB-T2A-PuroR
Plasmid#106249PurposeLentiviral vector with puro selection for expression of S. aureus dCas9-KRABDepositorInsertdead S. aureus Cas9 KRAB T2A PuroR
UseCRISPR and LentiviralTagsHA-NLS and NLS-KRABExpressionMammalianMutationD10A and N580APromoterhUbCAvailable sinceMay 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSpCTRE-CD4
Plasmid#114010PurposeDox-inducible Cas9 expression with a CD4 selection markerDepositorInsertSpCas9
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMarch 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
AAV CMV-dSaCas9-KRAB-bGHpA
Plasmid#106219PurposeAAV expressing deactivated S. aureus Cas9 fused to a KRAB repressorDepositorInsertdead S. aureus Cas9 KRAB
UseAAV and CRISPRTagsHA-NLSExpressionMammalianMutationD10A and N580APromoterCMVAvailable sinceMay 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
1GFP/RNase H1 WT
Plasmid#174447PurposeExpress GFP-tagged RNase H1 WT in E. coli.DepositorInsertRNase H1 (RNASEH1 Human)
UseTagsHis-tag, GFPExpressionBacterialMutationPromoterT7 promoterAvailable sinceSept. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCI H2B-RFP
Plasmid#92398PurposeUbiquitous expression plasmid, contains CAG promoter (CMV immediate early enhancer and chicken beta actin promoter), MCS and IRES controlled Histone2B-mRFP1 reporter.DepositorInsertIRES H2B RFP
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceAug. 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLJM60 FLAG-RAB35 wt
Plasmid#107104PurposeLentiviral vector that codes for FLAG-RAB35 wildtype (human) protein. Puromycin resistance for mammalian selection and ampicillin resistance for growth in Stbl3 cells.DepositorInsertRAB35 (RAB35 Human)
UseLentiviralTagsFLAG tagExpressionMammalianMutationwildtypePromoterCMVAvailable sinceMarch 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-N2-2XNLS-RNaseH1 delta 1-27 (D210N)
Plasmid#196703PurposePlasmid for transient mammalian expression of catalytically inactive RNase H1 mutant.DepositorInserthuman RNaseH1 D210N
UseTagsExpressionMammalianMutationD210N, catalytically inactivePromoterAvailable sinceFeb. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLJM60 FLAG-RAB35 S22N
Plasmid#107106PurposeLentiviral vector that codes for FLAG-RAB35 S22N (human) protein. Puromycin resistance for mammalian selection and ampicillin resistance for growth in Stbl3 cells.DepositorInsertRAB35 (RAB35 Human)
UseLentiviralTagsFLAG tagExpressionMammalianMutationPromoterCMVAvailable sinceMarch 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-dCas9
Plasmid#47106PurposeExpresses inactivated S. pyogenes dCas9 (D10A, H840A) in mammalian cellsDepositorInsertdCas9
UseCRISPRTagsFlag and SV40 NLSExpressionMammalianMutationD10A, H840A (catalytically inactive)PromoterCMVAvailable sinceNov. 14, 2013AvailabilityAcademic Institutions and Nonprofits only -
pLVX-RNaseHI-NES-EGFP
Plasmid#196701PurposePlasmid for stable expression of wild type bacterial RNase HI tagged with NES and EGFP that can be used to specifically degrade the DNA-RNA hybrids in the cytoplasm.DepositorInsertbacterial RNaseHI
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMarch 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMRE-Tn7-145
Plasmid#118561PurposeminiTn7 plasmid to deliver constitutively expressed fluorescent protein genes in bacteriaDepositorInsertmScarlet-I
UseMinitn7 delivery vectorTagsExpressionBacterialMutationPromoterAvailable sinceOct. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
Orai1-GCaMP6f
Plasmid#73564PurposeGreen fluorescent reporter of Orai1-associated calcium influxDepositorInsertOrai1-GCaMP6f (ORAI1 Synthetic, Human)
UseTagsGCaMP6fExpressionMammalianMutationPromoterCMV Immediate EarlyAvailable sinceMarch 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
LifeAct-14 (LA-14)
Plasmid#158750PurposeLentiviral plasmid for expression of shorter LifeAct variant (up till 14 amino acids) fused with carboxy terminus eGFP in mammalian cells.DepositorInsertLifeAct-14
UseLentiviralTagseGFPExpressionMammalianMutationDeletion mutation for last three amino acids from…PromoterCMV immediate earlyAvailable sinceSept. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTet-O-NEUROG1-T2A-PuroR
Plasmid#162339PurposeLentiviral expression of NEUROG1 under the control of the TetON promoterDepositorInsertNEUROG1 (NEUROG1 Human)
UseLentiviralTagsT2A-PuroRExpressionMammalianMutationPromoterTet-ONAvailable sinceFeb. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLenti Lifeact-iRFP670 BlastR
Plasmid#84385PurposeLentiviral expression of iRFP670-tagged Lifeact with Blasticidin selection in cells including neuronsDepositorInsertLifeact
UseLentiviralTagsiRFP670ExpressionMutationPromoterCMV immediate earlyAvailable sinceJan. 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCI H2B-GFP
Plasmid#92399PurposeUbiquitous expression plasmid, contains CAG promoter (CMV immediate early enhancer and chicken beta actin promoter), MCS and IRES controlled Histone2B-EGFP reporter.DepositorInsertIRES H2B EGFP
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceAug. 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-FLAG-SOX4
Plasmid#110360PurposeMammalian expression of FLAG-tagged SOX4DepositorInsertSOX4 (SOX4 Human)
UseTagsFLAGExpressionMammalianMutationlacking 5' UTRPromoterAvailable sinceMarch 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pWMD23
Plasmid#140006PurposeExpression of ORF1 (Shuffle 1 NLS) and Pong TPase (L418A, L420A)DepositorInsertsORF1 Shuffle 1 NLS
Pong TPase LA
UseTagsExpressionPlantMutationNES sequence mutated L418A, L420A and Ping N-term…PromoterAvailable sinceJan. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMCherry F8-2
Plasmid#186034PurposeExpress VHH F8-2 as a mCherry fusion in mammalian cellsDepositorInsertVHH F8-2
UseTagsmCherryExpressionMammalianMutationPromoterCMV immediate earlyAvailable sinceJuly 1, 2022AvailabilityAcademic Institutions and Nonprofits only