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Plasmid#84751PurposeExpresses human codon-optimized LbCpf1 protein and blasticidin resistance from EFS promoter. Lentiviral backbone.DepositorInsertLbCpf1
UseCRISPR and LentiviralTagsNLS-3xHA (C terminal on insert)ExpressionMammalianMutationPromoterEFS-NSAvailable sinceDec. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3_NSnoFbox-vhhGFP4
Plasmid#35580DepositorInsertNSnoFbox-vhhGFP4 (slmb Fly)
UseTagsExpressionMammalianMutationF box domain of slmb deleted.PromoterAvailable sinceApril 3, 2012AvailabilityAcademic Institutions and Nonprofits only -
pBMN-2xFKBP-mEGFP-NDP52
Plasmid#110465Purposeretrovirus construct for stable expressing mEGFP tagged NDP52DepositorInsertNDP52 (CALCOCO2 Human)
UseRetroviral; RetrovirusTagsmEGFPExpressionMammalianMutationPromoterAvailable sinceJan. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pWPI_SPbFGF
Plasmid#25812DepositorInsertpWPI_SPbFGF (FGF2 Human)
UseLentiviralTagsExpressionMutationIg signal peptide in N-terminal of human FGF2PromoterAvailable sinceJan. 24, 2011AvailabilityAcademic Institutions and Nonprofits only -
pHS0806 His14-bdSUMO-AwaIscB
Plasmid#176539PurposeBacterial expression of His14-bdSUMO-AwaIscBDepositorInsertAwaIscB
UseCRISPRTags14xHis-bdSUMO tagExpressionBacterialMutationPromoterT7Available sinceOct. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
MigR1-AE
Plasmid#12431DepositorUseRetroviralTagsHAExpressionMammalianMutationPromoterAvailable sinceJune 2, 2010AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3_HMOX1-V5-APEX2
Plasmid#85823PurposeExpressed C-term tagged APEX2 on HMOX1 in mammalian cellDepositorInsertHMOX1-V5-APEX2 (HMOX1 Synthetic, Human)
UseTagsAPEX2 and V5ExpressionMammalianMutationPromoterCMV-FAvailable sinceMarch 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Vamp3-pHluorin
Plasmid#190152PurposeExpresses (pH-sensitive) super ecliptic pHluorin-tagged rat Vamp3 for imaging exocytosisDepositorInsertVamp3 (Vamp3 Rat)
UseAAVTagssuperecliptic pHluorinExpressionMammalianMutationPromoterCMVAvailable sinceDec. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGMCK-T38S38-750-luxAB-DAS+4
Plasmid#49518PurposeKanamycin resistant; expresses luxAB-DAS+4 under the control of a promoter containing PtetO-4C5G; constitutively expresses reverse TetR (tetR38); integrates into the mycobacteriophage att-L5 siteDepositorInsertT38S8-P750-luxAB-DAS+4
UseTagsDAS+4 (AANDENYSENYADAS)ExpressionBacterialMutationPromoterP750Available sinceJan. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
px335-sgRNA-EF(Cas9n)-Dnmt3b-R
Plasmid#122333PurposeExpresses sgRNA targeting mouse Dnmt3b and Cas9 nickase in mammalian cellsDepositorInsertsgRNA for mouse Dnmt3b
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV_12xTEAD_luc2
Plasmid#173004Purposefirefly luciferase reporter vector with 12x clustered TEAD binding sitesDepositorInsert12xTEAD-luc2
UseAAV and LuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMKO.1 puro p53 shRNA 2
Plasmid#10672DepositorInsertp53 shRNA (TP53 Human)
UseRNAi and RetroviralTagsExpressionMammalianMutationPromoterAvailable sinceJan. 6, 2006AvailabilityAcademic Institutions and Nonprofits only -
TIA1 RRM-mCherry-iLIDx6
Plasmid#103782Purposeexpresses iPOLYMER component in mammalian cellsDepositorInsertTIA1 RRM-mCherry-iLIDx6
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceDec. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
eSpCas9-EF-Gsk3b(new)
Plasmid#122341PurposeExpresses sgRNA targeting mouse Gsk3b and eSpCas9 in mammalian cellsDepositorInsertsgRNA for mouse Gsk3b
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRK5 FLAG DEPTOR (PDZ domain)
Plasmid#21701DepositorInsertDEPTOR PDZ domain (DEPTOR Human)
UseTagsFLAGExpressionMammalianMutationPDZ domain onlyPromoterAvailable sinceJuly 28, 2009AvailabilityAcademic Institutions and Nonprofits only -
pHS1543
Plasmid#205963PurposeNZ_CP025815 DinG HNH Cascade proteins operon (E. coli codon optimized) with His14-tagged Csf3 in pET45b(+)DepositorInsertDinG HNH Cascade with His14-tagged Csf3
UseTags14xHis on Csf3ExpressionBacterialMutationPromoterT7Available sinceNov. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRK5 FLAG DEPTOR (DEP domains)
Plasmid#21700DepositorInsertDEPTOR DEP domains (DEPTOR Human)
UseTagsFLAGExpressionMammalianMutationDEP domains onlyPromoterAvailable sinceJuly 28, 2009AvailabilityAcademic Institutions and Nonprofits only -
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pCambia2300 - ProRUBY:RUBY-Citrine
Plasmid#128796PurposeFluorescent reporter for subcellular localisation and expression analysis of RUBYDepositorInsertRUBY PARTICLES IN MUCILAGE (AT1G19900 Mustard Weed)
UseTagsCitrineExpressionPlantMutationPromoterProRUBYAvailable sinceSept. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
MBC2-null
Plasmid#162749PurposeMSCV backbone with murine TCR-alpha signal peptide and murine TCR-beta2 constant region (TRBC2) for expression of TCR genesDepositorInsertmurine TCR-alpha signal peptide and murine TCR-beta2 constant region (TRBC2)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only