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Plasmid#159784PurposeS. pyogenes sgRNA collocated with pegRNA targeting human EMX1 geneDepositorInsertspacer of sgRNA targeting EMX1gene (EMX1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFX-Lifeact-EGFP
Plasmid#174477PurposeExpress EGFP with actin-targeting sequence in mammalian cellsDepositorInsertEGFP with actin-targeting sequence
UseTagsLifeact (N-terminal 17 amino acids from S. Cerevi…ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFX-Lifeact-SECFP
Plasmid#174476PurposeExpress SECFP with actin-targeting sequence in mammalian cellsDepositorInsertSECFP with actin-targeting sequence
UseTagsLifeact (N-terminal 17 amino acids from S. Cerevi…ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFX-Lifeact-Sirius
Plasmid#174475PurposeExpress Sirius with actin-targeting sequence in mammalian cellsDepositorInsertSirius with actin-targeting sequence
UseTagsLifeact (N-terminal 17 amino acids from S. Cerevi…ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLEX_307-SOD1WT
Plasmid#83444PurposeMammalian expression of SOD1WTDepositorInsertSOD1 (SOD1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF-1αAvailable sinceNov. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLEX_307-SOD1E133Δ
Plasmid#83445PurposeMammalian expression of SOD1E133Δ.DepositorInsertSOD1 (SOD1 Human)
UseLentiviralTagsExpressionMammalianMutationE133ΔPromoterEF-1αAvailable sinceNov. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSOD1E133K-AcGFP1
Plasmid#83442PurposeExpresses SOD1E133K in mammalian cells.DepositorInsertSOD1 (SOD1 Human)
UseTagsAcGFP1ExpressionMammalianMutationE133KPromoterCMVAvailable sinceNov. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLEX_307-SOD1E133insTT
Plasmid#83446PurposeMammalian expression of SOD1E133insTT.DepositorInsertSOD1 (SOD1 Human)
UseLentiviralTagsExpressionMammalianMutationE133insTT (after GA)PromoterEF-1αAvailable sinceNov. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLEX_307-SOD1E133K
Plasmid#83447PurposeMammalian expression of SOD1E133KDepositorInsertSOD1 (SOD1 Human)
UseLentiviralTagsExpressionMammalianMutationE133KPromoterAvailable sinceNov. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSOD1E133insTT-AcGFP1
Plasmid#83441PurposeExpresses SOD1E133insTT in mammalian cells.DepositorInsertSOD1 (SOD1 Human)
UseTagsAcGFP1ExpressionMammalianMutationE133insTTPromoterCMVAvailable sinceNov. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSOD1E133Δ-AcGFP1
Plasmid#83419PurposeExpresses SOD1E133Δ in mammalian cells.DepositorInsertSOD1 (SOD1 Human)
UseTagsAcGFP1ExpressionMammalianMutationE133 deletionPromoterCMVAvailable sinceNov. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV-syn-FLEX-splitTVA-EGFP-tTA (AAV1)
Viral Prep#100798-AAV1PurposeReady-to-use AAV1 particles produced from pAAV-syn-FLEX-splitTVA-EGFP-tTA (#100798). In addition to the viral particles, you will also receive purified pAAV-syn-FLEX-splitTVA-EGFP-tTA plasmid DNA. Helper virus for monosynaptic tracing with rabies virus; to be coinjected with pAAV-TREtight-mTagBFP2-B19G (Addgene#100799). These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagsEGFP (Cre-dependent)Available sinceDec. 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-TREtight-mTagBFP2-B19G (AAV1)
Viral Prep#100799-AAV1PurposeReady-to-use AAV1 particles produced from pAAV-TREtight-mTagBFP2-B19G (#100799). In addition to the viral particles, you will also receive purified pAAV-TREtight-mTagBFP2-B19G plasmid DNA. Helper virus for monosynaptic tracing with rabies virus; to be coinjected with pAAV-syn-FLEX-splitTVA-EGFP-tTA (Addgene# 100798). These AAV preparations are suitable purity for injection into animals.DepositorPromoterTREtightTagsBFP (in presence of Cre and 100798)Available sinceDec. 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
hSyn-DIO-somBiPOLES-mCerulean (AAV9)
Viral Prep#154951-AAV9PurposeReady-to-use AAV9 particles produced from hSyn-DIO-somBiPOLES-mCerulean (#154951). In addition to the viral particles, you will also receive purified hSyn-DIO-somBiPOLES-mCerulean plasmid DNA. Synapsin-driven, Cre-dependent expression of soma-targeted BiPOLES for optogenetic inhibition (blue light) and activation (red light). These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagsmCerulean (Cre-dependent)Available sinceSept. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
CaMKII-somBiPOLES-mCerulean (AAV9)
Viral Prep#154948-AAV9PurposeReady-to-use AAV9 particles produced from CaMKII-somBiPOLES-mCerulean (#154948). In addition to the viral particles, you will also receive purified CaMKII-somBiPOLES-mCerulean plasmid DNA. CamKII-driven expression of soma-targeted BiPOLES for optogenetic inhibition (blue light) and activation (red light). These AAV preparations are suitable purity for injection into animals.DepositorPromoterCaMKIIa(0.4)TagsmCeruleanAvailable sinceSept. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
CaMKII-somBiPOLES-mCerulean (AAV5)
Viral Prep#154948-AAV5PurposeReady-to-use AAV5 particles produced from CaMKII-somBiPOLES-mCerulean (#154948). In addition to the viral particles, you will also receive purified CaMKII-somBiPOLES-mCerulean plasmid DNA. CamKII-driven expression of soma-targeted BiPOLES for optogenetic inhibition (blue light) and activation (red light). These AAV preparations are suitable purity for injection into animals.DepositorPromoterCaMKIIa(0.4)TagsmCeruleanAvailable sinceSept. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
hSyn-DIO-somBiPOLES-mCerulean (AAV5)
Viral Prep#154951-AAV5PurposeReady-to-use AAV5 particles produced from hSyn-DIO-somBiPOLES-mCerulean (#154951). In addition to the viral particles, you will also receive purified hSyn-DIO-somBiPOLES-mCerulean plasmid DNA. Synapsin-driven, Cre-dependent expression of soma-targeted BiPOLES for optogenetic inhibition (blue light) and activation (red light). These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagsmCerulean (Cre-dependent)Available sinceSept. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSFFV_mNG2(11)1-10
Plasmid#82610PurposeLentiviral vector of constitutive expression of mNeonGreen2(1-10)DepositorInsertmNeonGreen2(1-10)
UseLentiviralTagsExpressionMammalianMutationK128M, S142T, R150M, G172V and K213MPromoterSFFVAvailable sinceMay 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1_sfCherry2(1-10)
Plasmid#82602PurposeExpresses sfCherry2(1-10) fusion in mammalian cellsDepositorInsertsfCherry2(1-10)
UseTagsExpressionMammalianMutationchanged Glu 118 to Gln, changed Thr 128 to IlePromoterCMVAvailable sinceJan. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-TFEB-WT-MYC (#647)
Plasmid#99955Purposemammalian expressionDepositorInsertTFEB (TFEB Human)
UseTags6xHis and MycExpressionMammalianMutationPromoterAvailable sinceNov. 7, 2017AvailabilityAcademic Institutions and Nonprofits only