Addgene

We narrowed to 6,544 results for: tev

Showing: 4721 - 4740 of 6544 results

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pFastBac-hDcr-K70A.

Plasmid

#89145

Purpose

Expression of helicase mutant of human Dicer in Sf9 cells

Depositor

Jennifer Doudna

Article

MacRae et al Proc Natl Acad Sci U S A. 2008 Jan 15;10

Insert

hDicer (DICER1 Human)

Use

Tags

His6-tev

Expression

Insect

Mutation

K70A

Promoter

Available since

April 25, 2017

Availability

Academic Institutions and Nonprofits only

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pJExpress401-KDAC8 D101R

Plasmid

#224349

Purpose

Expresses human KDAC8 (HDAC8) D101R in E. coli

Depositor

Terry Watt

Article

Toro et al Biochemistry. 2021 Aug 24;60(33):2524-25

Insert

KDAC8 (HDAC8 Human)

Use

Tags

TEV-cleavable His6

Expression

Bacterial

Mutation

Aspartate 101 mutated to arginine

Promoter

T5

Available since

Oct. 28, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pET24a_SMOXdel

Plasmid

#186785

Purpose

C-terminal His-Tag and a TEV cleaveage site, deletion of 8 residues (503-512) to transform into E. coli BL21 (DE3) cells

Depositor

Sandra Gabelli

Article

Dunston et al Acta Naturae. 2020 Jul-Sep;12(3):140-144

Insert

Spermine oxidase (SMOX Human)

Use

Tags

His tag

Expression

Bacterial

Mutation

deleted amino acids 503-512

Promoter

Available since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pJExpress401-KDAC8 D101A

Plasmid

#224347

Purpose

Expresses human KDAC8 (HDAC8) D101A in E. coli

Depositor

Terry Watt

Article

Toro et al Biochemistry. 2021 Aug 24;60(33):2524-25

Insert

KDAC8 (HDAC8 Human)

Use

Tags

TEV-cleavable His6

Expression

Bacterial

Mutation

Aspartate 101 mutated to alanine

Promoter

T5

Available since

Oct. 22, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pFastBacI-KDAC8 D101E

Plasmid

#224350

Purpose

Expresses human KDAC8 (HDAC8) D101E in insect cells

Depositor

Terry Watt

Article

Toro et al Biochemistry. 2021 Aug 24;60(33):2524-25

Insert

KDAC8 (HDAC8 Human)

Use

Tags

TEV-cleavable His6

Expression

Insect

Mutation

Aspartate 101 mutated to glutamate

Promoter

Polyhedrin

Available since

Oct. 22, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pFastBacI-KDAC6 H216A

Plasmid

#224351

Purpose

Expresses human KDAC6 (HDAC6) H216A in insect cells

Depositor

Terry Watt

Article

Toro et al Biochemistry. 2023 May 2;62(9):1464-1483

Insert

KDAC6 (HDAC6 Human)

Use

Tags

TEV-cleavable His6

Expression

Insect

Mutation

Histidine 216 mutated to alanine

Promoter

Polyhedrin

Available since

Oct. 22, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pFastBacI-KDAC6 H611A

Plasmid

#224352

Purpose

Expresses human KDAC6 (HDAC6) H611A in insect cells

Depositor

Terry Watt

Article

Toro et al Biochemistry. 2023 May 2;62(9):1464-1483

Insert

KDAC6 (HDAC6 Human)

Use

Tags

TEV-cleavable His6

Expression

Insect

Mutation

Histidine 611 mutated to alanine

Promoter

Polyhedrin

Available since

Oct. 22, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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hCD12

Plasmid

#225541

Purpose

Expresses human histone deacetylase 6 (HDAC6) catalytic domain 1 and 2 (CD12), also known as hCD12, in Escherichia coli cells.

Depositor

David Christianson

Article

Hai et al Nat Chem Biol. 2016 Sep;12(9):741-7. doi

Insert

Human Histone Deacetylase 6 Catalytic Domain 1 and 2 (HDAC6 Human)

Use

Tags

His6-MBP tag with cleavable TEV site

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pWzyE-His

Plasmid

#196669

Purpose

Expression of C-terminally His10-tagged WzyE from Pectobacterium atrosepticum in E.coli

Depositor

James Naismith

Article

Weckener et al Open Biol. 2023 Mar;13(3):220373. doi: 1

Insert

WzyE

Use

Tags

TEV cleavable His10

Expression

Bacterial

Mutation

Promoter

araBAD

Available since

July 19, 2024

Availability

Academic Institutions and Nonprofits only

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SCML2_3

Plasmid

#220878

Purpose

Baculovirus expression for structure determination; may not be full ORF

Depositor

Cheryl Arrowsmith

Article

Nady et al J Mol Biol. 2012 Nov 9;423(5):702-18. do

Insert

SCML2 (SCML2 Human)

Use

Tags

6xHis-TEV

Expression

Bacterial

Mutation

Promoter

T7

Available since

June 20, 2024

Availability

Academic Institutions and Nonprofits only

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pET28_human-pro-IL-18_E192K_D193K

Plasmid

#214322

Purpose

Protein overexpression in bacteria

Depositor

Jonathan Kagan

Article

Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

Insert

IL18 (IL18 Human)

Use

Tags

His-TEV

Expression

Bacterial

Mutation

E192K_D193K

Promoter

T7

Available since

April 10, 2024

Availability

Academic Institutions and Nonprofits only

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pET28_human-pro-IL-18_I48N

Plasmid

#214323

Purpose

Protein overexpression in bacteria

Depositor

Jonathan Kagan

Article

Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

Insert

IL18 (IL18 Human)

Use

Tags

His-TEV

Expression

Bacterial

Mutation

I48N

Promoter

T7

Available since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

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pFastBac_human-IL-18

Plasmid

#214338

Purpose

Protein overexpression in bacteria

Depositor

Jonathan Kagan

Article

Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

Insert

IL18 (IL18 Human)

Use

Tags

His-TEV

Expression

Bacterial

Mutation

Promoter

T7

Available since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a_mouse-Pro-IL-18_humanized-triple-mutant

Plasmid

#214339

Purpose

Protein overexpression in bacteria

Depositor

Jonathan Kagan

Article

Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

Insert

IL18 (Il18 Mouse)

Use

Tags

His-TEV

Expression

Bacterial

Mutation

ENGD29DDEN_N36Y_H41E

Promoter

T7

Available since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pET28_human-pro-IL-18_LES33AAA

Plasmid

#214326

Purpose

Protein overexpression in bacteria

Depositor

Jonathan Kagan

Article

Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

Insert

IL18 (IL18 Human)

Use

Tags

His-TEV

Expression

Bacterial

Mutation

LES33AAA

Promoter

T7

Available since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pET28_human-pro-IL-18_DDEN29ENGD

Plasmid

#214325

Purpose

Protein overexpression in bacteria

Depositor

Jonathan Kagan

Article

Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

Insert

IL18 (IL18 Human)

Use

Tags

His-TEV

Expression

Bacterial

Mutation

DDEN29ENGD

Promoter

T7

Available since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pET28_human-pro-IL-18_V47N_I48N

Plasmid

#214324

Purpose

Protein overexpression in bacteria

Depositor

Jonathan Kagan

Article

Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

Insert

IL18 (IL18 Human)

Use

Tags

His-TEV

Expression

Bacterial

Mutation

V47N_I48N

Promoter

T7

Available since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pFastBac_human-pro-IL-18

Plasmid

#214337

Purpose

Protein overexpression in bacteria

Depositor

Jonathan Kagan

Article

Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

Insert

IL18 (IL18 Human)

Use

Tags

His-TEV

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28a_Pro-IL-18_rat

Plasmid

#214333

Purpose

Protein overexpression in bacteria

Depositor

Jonathan Kagan

Article

Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

Insert

IL18 (Il18 Rat)

Use

Tags

His-TEV and Myc

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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HA-Halo-H2DBD

Plasmid

#200206

Purpose

Expression of truncated HSF2 (DBD)

Depositor

Sheila Teves

Article

Price et al Nucleic Acids Res. 2023 Apr 28:gkad304.

Insert

HA-Halo-H2DBD (Hsf2 Mouse)

Use

Tags

Halo; HA

Expression

Mammalian

Mutation

HSF2 (1-366; 15 bp linker; 972-1017)

Promoter

EF_1alpha

Available since

May 30, 2023

Availability

Academic Institutions and Nonprofits only

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