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Plasmid#115818PurposeEncodes codon optimized 3xFLAG-SIVLST Vpr (AF188116) and puromycin resistance proteinDepositorInsertSIVLST Vpr (AF188116)
UseLentiviralTags3x-FLAGExpressionMutationPromoterSFFVAvailable sinceJan. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pscALPS-SIV-MND1 vpr
Plasmid#115817PurposeEncodes codon optimized 3xFLAG-SIVMND1 Vpr (M27470) and puromycin resistance proteinDepositorInsertSIVMND1 Vpr (M27470)
UseLentiviralTags3x-FLAGExpressionMutationPromoterSFFVAvailable sinceJan. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJB02
Plasmid#111959PurposeExpresses GST-GFP-DHFR; construct generated for pull-down and localization experimentsDepositorInsertsmEGFP
eDHFR
UseTagsDHFRExpressionBacterialMutationeDHFR;residues 2-159 and enhanced GFP with monome…PromoterT7Available sinceAug. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-FLAG-SBP-STAG2 (S97X)
Plasmid#73964PurposeSTAG2 cDNA (CCDS43990) S97XDepositorInsertSTAG2 (STAG2 Human)
UseTagsFLAG-SBPExpressionMammalianMutationS97XPromoterAvailable sinceMarch 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pISH-Dlx3
Plasmid#74342PurposeUse to synthesize riboprobes for in situ hybridization (ISH) probe for Dlx3. Not for gene expression.DepositorInsertdistal-less homeobox 3 (Dlx3 Mouse)
UseTagsExpressionMutationPromoterAvailable sinceApril 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pISH-Lhx2
Plasmid#74337PurposeUse to synthesize riboprobes for in situ hybridization (ISH) probe for Lhx2. Not for gene expression.DepositorInsertLIM homeobox protein 2 (Lhx2 Mouse)
UseTagsExpressionMutationPromoterAvailable sinceMay 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
mSTIM1_1-310-CFP
Plasmid#69551PurposeExpresses a STIM1_1-310 fragment mammalian cellsDepositorInsertmSTIM1_1-310
UseTagsCyan Fluorescent Protein (CFP)ExpressionMammalianMutationPromoterCMVAvailable sinceOct. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK IRF9 3'UTR
Plasmid#19863DepositorInsertIRF9 3'UTR (IRF9 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 12, 2009AvailabilityAcademic Institutions and Nonprofits only -
pGEX PLCg1(N)-SH2
Plasmid#46470DepositorInsertPLCg1(N)
UseTagsGST and PreScissionExpressionBacterialMutationcontains amino acids 539-664PromoterTacAvailable sinceAug. 1, 2013AvailabilityAcademic Institutions and Nonprofits only -
3122 pSFFV-neo Bcl-XL MI-3
Plasmid#8811DepositorInsertBcl-XL MI-3 (BCL2L1 Human)
UseTagsExpressionMammalianMutationBH1 WGR-->WARPromoterAvailable sinceJuly 6, 2005AvailabilityAcademic Institutions and Nonprofits only -
YFP(1-158) in pcDNAI/Amp
Plasmid#60888PurposeThis vector attaches YFP(1-158) to the N-terminus of a protein.DepositorInsertYFP(1-158)
UseTagsExpressionMammalianMutationMet was substituted for Gln-69.PromoterCMVAvailable sinceJuly 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEYFP-N1-hRIP3-FL V460P
Plasmid#61379PurposeExpresses Human full length RIPK3 with V460P mutation in the mammalian cellsDepositorInsertFull length human RIP3 with V460Pmutation (RIPK3 Human)
UseTagsEYFPExpressionMammalianMutationchanged V460 to PPromoterAvailable sinceJan. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEYFP-N1-hRIP3-FL V458P
Plasmid#61377PurposeExpresses Human full length RIPK3 with V458P mutation in the mammalian cellsDepositorInsertFull length human RIP3 with V458Pmutation (RIPK3 Human)
UseTagsEYFPExpressionMammalianMutationchanged V458 to PPromoterAvailable sinceJan. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-ERKsubDD-Venus-NES
Plasmid#84630PurposeEncodes C-terminal (substrate) fragment of bimolecular ERK activity reporter (bimEKAR); cytosol targetedDepositorInsertERKsubDD-Venus-NES
UseTags6xHis, Nuclear export signal (NES), T7 tag (gene …ExpressionMammalianMutationPromoterCMVAvailable sinceOct. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-myc-mCE(K294A)
Plasmid#82461PurposeExpression of myc epitope and catalytic inactive form of murine mRNA Capping Enzyme (K294A) in mammalian cellsDepositorInsertmCE (K294A) (Rngtt Mouse)
UseTagsmycExpressionMammalianMutationLysine 294 changed to AlaninePromoterCMVAvailable sinceSept. 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO HIS DNAJA4
Plasmid#19548DepositorInsertDnaJ (Hsp40) homolog, subfamily A, member 4 (DNAJA4 Human)
UseTagsHisExpressionMammalianMutationPromoterAvailable sinceOct. 6, 2008AvailabilityAcademic Institutions and Nonprofits only -
pPAct2noUTR (GB0192)
Plasmid#68177PurposeProvides the Arabidopsis thaliana actin2 gene promoter (without 5' UTR) as a level 0 GoldenBraid partDepositorInsertAtAct2 promoter (no UTR)
UseSynthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceMarch 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJZC40
Plasmid#62334PurposesgRNA + 2x PP7 with mCherry for mammalian cellsDepositorInsertssgRNA + 2x PP7
mCherry
UseLentiviralTagsExpressionMammalianMutationTargets Tet3G, sequence: GTACGTTCTCTATCACTGATAPromoterCMV and U6Available sinceMay 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_NCK1-1/3
Plasmid#91472PurposeProtein expression and purification of human SH3 domain construct NCK1-1/3DepositorInsertNCK1-1/3 (NCK1 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pPAtUbq10 (GB0223)
Plasmid#68174PurposeProvides the Arabidopsis thaliana polyubiquitin 10 promoter as a level 0 GoldenBraid partDepositorInsertAtUbq10 promoter
UseSynthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceOct. 22, 2015AvailabilityAcademic Institutions and Nonprofits only