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pGEX Mist-SH2

Plasmid

#46454

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Mist (Clnk Mouse)

Use

Tags

AviTag, GST, and PreScission

Expression

Bacterial

Mutation

contains amino acids 300-435

Promoter

Tac

Available since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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CRY-GalVP16l (B694)

Plasmid

#92030

Purpose

expresses CRY2 (Full length, mutant NLS) fused to Gal4DNA (residues 1-147) fused to VP16 activation domain (long form)

Depositor

Chandra Tucker

Article

Pathak et al Nucleic Acids Res. 2017 Apr 20. doi: 10.

Insert

CRY2 (CRY2 Mustard Weed)

Use

Tags

GalBD-VP16

Expression

Mammalian

Mutation

NLS sequence of CRY2 mutated

Promoter

CMV

Available since

July 25, 2017

Availability

Academic Institutions and Nonprofits only

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pPHR-EYFP-NLS-VP16

Plasmid

#103822

Purpose

Soluble BLInCR transcription activator (transactivation domain of viral VP16 protein) that is recruited to 'localizer' sites upon blue light illumination

Depositor

Karsten Rippe

Article

Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

Insert

PHR-EYFP-VP16 (CRY2 Mustard Weed)

Use

Tags

Expression

Mammalian

Mutation

VP16: G126T (silent, G42G)

Promoter

CMV

Available since

Dec. 1, 2017

Availability

Academic Institutions and Nonprofits only

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psiCHECK2-CCNA2 LONG 3'UTR

Plasmid

#29472

Depositor

Judy Lieberman

Article

Lal et al Mol Cell. 2009 Sep 11. 35(5):610-25.

Insert

CCNA2 LONG 3'UTR (CCNA2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 27, 2011

Availability

Academic Institutions and Nonprofits only
pGEX6P1-HA-MEK1 CBM

Plasmid

#53163

Purpose

Expresses GST-HA tagged human MEK1 M187AH188AM230AH239A from bacterial expression vector

Depositor

Christopher Counter

Article

Brady et al Nature. 2014 Apr 9. doi: 10.1038/nature1

Insert

MEK1 (MAP2K1 Human)

Use

Tags

GST and HA

Expression

Bacterial

Mutation

M187A/H188A/M230A/H239A

Promoter

Available since

June 9, 2014

Availability

Academic Institutions and Nonprofits only
pGEX6P1-HA-MEK5 CBM-E

Plasmid

#53168

Purpose

Expresses GST-HA tagged human MEK5 L280AH281AM323AQ332A from bacterial expression vector

Depositor

Christopher Counter

Article

Brady et al Nature. 2014 Apr 9. doi: 10.1038/nature1

Insert

MEK5 (MAP2K5 Human)

Use

Tags

GST and HA

Expression

Bacterial

Mutation

L280A/H281A/M323A/Q332A

Promoter

Available since

Sept. 29, 2014

Availability

Academic Institutions and Nonprofits only
pCIN4-EZOD

Plasmid

#44977

Depositor

Robert Oshima

Article

Galang et al J Biol Chem. 1996 Apr 5;271(14):7992-8.

Insert

Ets2 DBD (Ets2 Mouse)

Use

Tags

FLAG and SV40 NLS

Expression

Mammalian

Mutation

Ets2 DNA binding domain (AA 332-468) only = domin…

Promoter

CMV

Available since

May 28, 2013

Availability

Academic Institutions and Nonprofits only
integrin alpha9 W999A pcDNA1 neo

Plasmid

#13589

Depositor

Dean Sheppard

Article

Young et al Mol Biol Cell. 2001 Oct . 12(10):3214-25

Insert

integrin alpha 9 W999A (ITGA9 Human)

Use

Tags

Expression

Mammalian

Mutation

W999A

Promoter

Available since

Jan. 8, 2007

Availability

Academic Institutions and Nonprofits only

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pYTSK01K_0G7

Plasmid

#177296

Purpose

genomic integration of genes (inserted in I-SceI-site) via transposon Tn7 with GmR

Depositor

Karl-Erich Jaeger

Article

Tiso et al Front Bioeng Biotechnol. 2020 Aug 20;8:9

Insert

PaacC1-aacC1

Use

Synthetic Biology; Yeast expression, tn7 genomic …

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 31, 2024

Availability

Academic Institutions and Nonprofits only

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NEIL1-mCherry

Plasmid

#215133

Purpose

Expresses NEIL1-mCherry in mammalian cells from a retroviral vector.

Depositor

Michele Pagano

Article

Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

Insert

NEIL1 (NEIL1 Human)

Use

Retroviral

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 17, 2024

Availability

Academic Institutions and Nonprofits only

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pBABE-NLS-HA-TurboID

Plasmid

#215073

Purpose

Expresses NLS-HA-TurboID in mammalian cells from a retroviral vector.

Depositor

Michele Pagano

Article

Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

Insert

TurboID (birA )

Use

Retroviral

Tags

NLS-HA

Expression

Mammalian

Mutation

Promoter

LTR

Available since

April 8, 2024

Availability

Academic Institutions and Nonprofits only

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TurboID-13x-EV

Plasmid

#215086

Purpose

Expresses TurboID-13x[linker] in mammalian cells from a retroviral vector.

Depositor

Michele Pagano

Article

Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

Insert

TurboID (birA )

Use

Retroviral

Tags

13x [linker]

Expression

Mammalian

Mutation

Promoter

LTR

Available since

April 8, 2024

Availability

Academic Institutions and Nonprofits only

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mPlum-PCNA-PuroR

Plasmid

#215046

Purpose

Expresses mPlum-PCNA in mammalian cells from a retroviral vector.

Depositor

Michele Pagano

Article

Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

Insert

PCNA (PCNA Human)

Use

Retroviral

Tags

mPlum

Expression

Mammalian

Mutation

Promoter

LTR

Available since

April 5, 2024

Availability

Academic Institutions and Nonprofits only

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mCerulean-PCNA-HygroR

Plasmid

#215064

Purpose

Expresses mCerulean-PCNA in mammalian cells from a retroviral vector.

Depositor

Michele Pagano

Article

Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

Insert

PCNA (PCNA Human)

Use

Retroviral

Tags

mCerulean

Expression

Mammalian

Mutation

Promoter

LTR

Available since

April 5, 2024

Availability

Academic Institutions and Nonprofits only

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mPlum-PCNA-HygroR

Plasmid

#215047

Purpose

Expresses mPlum-PCNA in mammalian cells from a retroviral vector.

Depositor

Michele Pagano

Article

Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

Insert

PCNA (PCNA Human)

Use

Retroviral

Tags

mPlum

Expression

Mammalian

Mutation

Promoter

LTR

Available since

April 5, 2024

Availability

Academic Institutions and Nonprofits only

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pET28-His6-SUMO-cSrc-SH2-AviTag

Plasmid

#214230

Purpose

Bacterial expression of SH2 domain of the cSrc kinase with N-terminal Thrombin-cleavable 6xHis tag and SUMO and C-terminal AviTag for Streptavidin bead functionalization for binder selections

Depositor

Neel Shah

Article

Li et al Elife. 2023 Mar 16;12:e82345. doi: 10.75

Insert

cSrc kinase SH2 domain (SRC Human)

Use

Tags

6xHis, AviTag, and SUMO

Expression

Bacterial

Mutation

Promoter

T7

Available since

April 4, 2024

Availability

Academic Institutions and Nonprofits only

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mCherry-XRCC1

Plasmid

#215142

Purpose

Expresses mCherry-XRCC1 in mammalian cells from a retroviral vector.

Depositor

Michele Pagano

Article

Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

Insert

XRCC1 (XRCC1 Human)

Use

Retroviral

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 3, 2024

Availability

Academic Institutions and Nonprofits only

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mCherry-MUTHY

Plasmid

#215132

Purpose

Expresses mCherry-MUTHY in mammalian cells from a retroviral vector.

Depositor

Michele Pagano

Article

Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

Insert

MUTHY (MUTYH Human)

Use

Retroviral

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 1, 2024

Availability

Academic Institutions and Nonprofits only

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NEIL2-mCherry

Plasmid

#215134

Purpose

Expresses NEIL2-mCherry in mammalian cells from a retroviral vector.

Depositor

Michele Pagano

Article

Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

Insert

NEIL2 (NEIL2 Human)

Use

Retroviral

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 1, 2024

Availability

Academic Institutions and Nonprofits only

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mCherry-POLB

Plasmid

#215138

Purpose

Expresses mCherry-POLB in mammalian cells from a retroviral vector.

Depositor

Michele Pagano

Article

Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

Insert

POLB (POLB Human)

Use

Retroviral

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 1, 2024

Availability

Academic Institutions and Nonprofits only

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