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Plasmid#105340PurposeSynthetic biology; gateway destination vector assemblyDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 2, 2018AvailabilityAcademic Institutions and Nonprofits only
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AP568-3
Plasmid#70048Purposeco-expression of Cas9 and crRNA 589 target sequenceDepositorInsertsgRNA for dpy‐10
UseCRISPRTagsExpressionWormMutationPromoterAvailable sinceNov. 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3myc AR-LBD
Plasmid#89112Purposemammalian expression of human androgen receptor ligand binding domain amino acid residues 624-919 with N-terminal myc tagDepositorInserthAR ligand binding domain (AR Human)
UseTagsmycExpressionMammalianMutationcontains Ligand Binding Domain (624-919)PromoterCMVAvailable sinceApril 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
AP568-2
Plasmid#70047Purposeco-expression of Cas9 and crRNA 589 target sequenceDepositorInsertsgRNA for dpy‐10
UseCRISPRTagsExpressionWormMutationPromoterAvailable sinceOct. 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
VKG1-gRNA-pX330
Plasmid#108671PurposeCleavage of VKG1 sequence by CRISPR/Cas9DepositorInsertgRNA for VKG1 sequence
UseCRISPRTagsExpressionMutationPromoterAvailable sinceMay 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCK011
Plasmid#105343PurposeYeast two hybridDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceAug. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCK012
Plasmid#105344PurposeYeast two hybridDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceAug. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pNAS1B split mCherry N-MESVD/TRAP(RK)-C
Plasmid#61967PurposeDuet plasmid for weak split mCherry assembly (as compared to N-ME(pS)VD/TRAP(RK)-C). N-mCherry is fused to the peptide MESVDDepositorInsertsN-mCherry-MESVD
TRAP(RK)-C-mCherry
UseTagsExpressionBacterialMutationPromoterPBAD and PLtetOAvailable sinceFeb. 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-MAVS-M142A-invitro(E13)
Plasmid#52017PurposeUsed for in vitro expression of the human MAVS CDS containing a point mutation M142ADepositorInsertMAVS-M142A
UseTagsExpressionMammalianMutationchanged Met 142 to AlaPromoterAvailable sinceMarch 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
pRibo-APEX2
Plasmid#111698PurposeTheophylline-inducible expression of APEX2 from a mycobacterial shuttle plasmid (episomal)DepositorInsertAPEX2
UseTagsV5ExpressionBacterialMutationPromoterhsp60-riboAvailable sinceSept. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJOG174
Plasmid#105396PurposeSynthetic biologyDepositorInsertmVenus (GFP variant)
UseSynthetic BiologyTagsExpressionMutationBsaI/ BpiI restriction sites removedPromoterAvailable sinceAug. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJOG176
Plasmid#105392PurposeSynthetic biologyDepositorInsertmEGFP (GFP variant)
UseSynthetic BiologyTagsExpressionMutationBsaI/ BpiI restriction sites removedPromoterAvailable sinceAug. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
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pAGM16467
Plasmid#105403PurposeSynthetic biologyDepositorInsert6xHis-NES(PKI)
UseSynthetic BiologyTagsExpressionMutationBsaI/ BpiI restriction sites removedPromoterAvailable sinceAug. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pVKG1-Puro
Plasmid#108670PurposePuromycin resistant cassette for knock-in by VIKING methodDepositorInsertPURO
UseCRISPR and Synthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTEI038
Plasmid#105353PurposeSynthetic biologyDepositorInsertpromotor G10-90
UseSynthetic BiologyTagsExpressionMutationBsaI/ BpiI restriction sites removedPromoterAvailable sinceMarch 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRibo-Sec-APEX2
Plasmid#111699PurposeTheophylline-inducible expression of periplasmic APEX2 from a mycobacterial shuttle plasmid (episomal)DepositorInsertAPEX2
UseTagsV5ExpressionBacterialMutationPromoterhsp60-riboAvailable sinceSept. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMV306-ribo-APEX2
Plasmid#111700PurposeTheophylline-inducible expression of APEX2 from a mycobacterial shuttle plasmid (integrating)DepositorInsertAPEX2
UseTagsV5ExpressionBacterialMutationPromoterhsp60-riboAvailable sinceSept. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRS416-PTEF1-MCP-3P-ECM33_intron
Plasmid#127601Purposelow-copy URA-selectable plasmid, 1xFLAG-MCP under TEF1 promoter, 3P-ECM33 intron inserted into URA3DepositorInsertECM33 intron with MS2 hairpins inserted near 3′ end of intron
UseTagsExpressionYeastMutationPromoterAvailable sinceApril 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRS416-PTEF1-MCP-5P-ECM33_intron
Plasmid#127599Purposelow-copy URA-selectable plasmid, 1xFLAG-MCP under TEF1 promoter, 5P-ECM33 intron inserted into URA3DepositorInsertECM33 intron with MS2 hairpins inserted near 5′ end of intron
UseTagsExpressionYeastMutationPromoterAvailable sinceApril 9, 2020AvailabilityAcademic Institutions and Nonprofits only