-
Plasmid#85613PurposeCas9-tracrRNA integration vector for Bacillus subtilisDepositorInsertcas9-tracrRNA
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLenti CMV GFP Neo (657-2)
Plasmid#17447Purpose3rd gen lentiviral eGFP expression vector, CMV promoter, NeoDepositorInsertGFP
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 12, 2009AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CaMKII-GAL4-iLight-VP16
Plasmid#170274PurposeNLS-Gal4(1-65)-iLight(human codon optimized)-VP16 construct expression in mammalian cells. pAAV vector.DepositorInsertNLS-Gal4(1-65)-iLight(human codon optimized)-VP16
UseAAVTagsExpressionMutationSee CommentsPromoterCaMKIIAvailable sinceJune 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
-
pDXinit
Plasmid#110101PurposeE.coli entry and expression vector for FX cloning system, N-terminal pelB signal sequence and C-terminal fusion to PIII for phage display using M13 phagesDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUC57-mitoTPNOX
Plasmid#87854PurposeThis plasmid contains human codon optimized sequence of Lactobacillus brevis mutant of a water-forming oxidase that can be used to increase NADP+/NADPH ratio in mammalian cells.DepositorInsertmitoTPNOX
UseTagsC-terminal FLAG tag with a linker and mitochondri…ExpressionBacterialMutationPromoterAvailable sinceAug. 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pmScarlet3-LaminB_C1
Plasmid#189776PurposeConstruct for labelling nuclear envelopes in mammaiian cells using mScarlet3-LaminB fusionDepositorInsertmScarlet3-LaminB
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
PM-B
Plasmid#209876PurposeDimerization dependent fluorescent protein B anchored to cytosolic face of the plasma membrane with the N-terminal targeting sequence of human GAP43DepositorInsertddGFP B
UseTagsTargeting domain of Gap43 MLCCMRRTKQVEKNDDQKIExpressionMammalianMutationPromoterCMVAvailable sinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLenti-mCherry-Mango II x 24
Plasmid#127587PurposeExpresses the mCherry-Mango II x 24 array from a TetON responsive promoterDepositorInsertmCherry-Mango II x24
UseTagsmCherryExpressionMammalianMutationPromoterpTRE Tet ONAvailable sinceMarch 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaM-4
Plasmid#172770PurposeBacterial expression of anti-mCherry nanobody LaM-4, with pelB leader and C-term free cysteine and 6xHIS tag.DepositorInsertLaM-4
UseTags6xHIS and pelB leader for periplasmic secretionExpressionBacterialMutationPromoterT7Available sinceAug. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
hCRISPRi_dual_1_2
Pooled Library#187246PurposeUltra-compact, human genome-wide CRISPRi library targeting each gene with a single element encoding a dual sgRNA cassette.DepositorSpeciesSyntheticUseLentiviralAvailable sinceSept. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBABE-zeo largeTgenomic
Plasmid#1778DepositorInsertlarge T and small T antigen (SV40gp6 simian virus)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceJune 23, 2005AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1_FASN
Plasmid#107138PurposeHis tagged human FASN cloned in pcDNA3.1 vector for expression in mammalian cellsDepositorInsertFatty acid synthase (FASN Human)
UseTagsHisExpressionMammalianMutationPromoterAvailable sinceMarch 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-mSmo
Plasmid#25395DepositorInsertmouse Smoothened cDNA (Smo Mouse)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceJune 22, 2010AvailabilityAcademic Institutions and Nonprofits only -
pLDLR-Luc mutSRE
Plasmid#14945DepositorInsertLDLR promoter mut SRE (LDLR Human)
UseLuciferaseTagsluciferaseExpressionMammalianMutationPoint mutation in the SRE-1 sequence of the LDL r…PromoterAvailable sinceMay 21, 2007AvailabilityAcademic Institutions and Nonprofits only -
pFETCh_Donor (EMM0021)
Plasmid#63934Purpose3X Flag with P2A-Neo for 3' tagging of endogenous proteinsDepositorHas ServiceCloning Grade DNATypeEmpty backboneUseTagsGlycine-Serine-3XFlag-P2A-NeoRExpressionMammalianMutationPromoterAvailable sinceJuly 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
Watermelon
Pooled Library#155257PurposeLineage tracing libraryDepositorExpressionMammalianUseLentiviralAvailable sinceOct. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
GFP_CD47_SU
Plasmid#65473PurposeGFP tagged CD47 with its own short 3'UTRDepositorInsertsSignal peptide of CD47
GFP
CD47 coding region continued and short 3’UTR.
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
LentiGuide_DKO
Plasmid#183193PurposePlasmid with two U6 promoters and two gRNA scaffolds that allows for inserting two gRNAs for combinatorial CRISPR Screen or double-knock-out (DKO) screenDepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6, mU6Available sinceAug. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHMGCS-Luc (aka: pES8)
Plasmid#14947DepositorInsertHMG CoA synthase promoter
UseLuciferaseTagsluciferaseExpressionMammalianMutationPromoterAvailable sinceMay 21, 2007AvailabilityAcademic Institutions and Nonprofits only