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Plasmid#186570Purposep15A ori, lacI, Ptac promoter, AmpR. Kringle YFP (Yellow Fluorescence Protein) expressionDepositorInsertYFP
UseTagsExpressionBacterialMutationPromotertac promoterAvailable sinceNov. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pVRa16_3622
Plasmid#49670DepositorInsertECF16_3622
UseSynthetic BiologyTagsHis6-PreScissionExpressionBacterialMutationCodon optimized for E.coliPromoterpT7-lacOAvailable sinceJan. 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hRREB1-RFP
Plasmid#22924DepositorInserthuman ras responsive element binding protein 1 driving DsRedExpress (RREB1 Human)
UseAAVTagsExpressionMammalianMutationPromoterAvailable sinceMay 25, 2010AvailabilityAcademic Institutions and Nonprofits only -
pCS2-zfKitlgb
Plasmid#168200PurposeExpress zebrafish Kitlgb, mRNA synthesisDepositorInsertKitlgb (kitlgb Zebrafish)
UseVertebrate expression (zebrafish, chicken, xenopu…TagsExpressionMammalianMutationI85V, V222I, and D233N- please see depositor comm…PromoterAvailable sinceNov. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBTK130
Plasmid#110584PurposeBTK Type 2 promoter for golden gate assemblyDepositorInsertT7 promoter with lac operator and RBS
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 20, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
BAT-lux
Plasmid#20890DepositorInsert7xTCF binding sites, Siamois minimal promoter
UseLuciferaseTagsExpressionMammalianMutation7 multimerized TCF-binding elements fused upstrea…PromoterAvailable sinceApril 22, 2009AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK-2-Smad5-3'UTR
Plasmid#61777PurposeLuciferase reporter containing the 3'UTR of mouse Smad5DepositorInsertmouse Smad5 3'UTR
UseLuciferaseTagsExpressionMutationPromoterAvailable sinceApril 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPR - Amplicon, BCR/ABL sgRNA 1
Plasmid#70659PurposeExpresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an intergenic BCR/ABL-targeting sgRNA element from U6 promoter. Lentiviral backboneDepositorInsertsgRNA against BCR/ABLamplicon found in CML-derived K562 cell line
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceOct. 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSEM232 - [Pmlc-1 | tagRFP-T (2x NLS)| cbr-tbb-2 UTR]
Plasmid#159898PurposeFluorescent co-injection marker to identify extrachromosomal arrays in C. elegansDepositorInsertPmlc-1 | tagRFP-T (2x NLS)| cbr-tbb-2 UTR
UseTagsExpressionWormMutationPromoterAvailable sinceOct. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBICI_2_strong
Plasmid#155168Purposesee pBICI_strong: exchanged origin of replication (pBR322) and introduced bom siteDepositorInsertsSucrose Phosphorylase from Bifidobacterium adolescentis (BAD_RS00415 Bifidobacterium adolescentis)
Cellobiose phosphorylase from Cellulomonas uda
UseSynthetic BiologyTagsHis Tag and Strep - Tag IIExpressionBacterialMutationPromoterAvailable sinceSept. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
R4pGWB601_UBQ10p-BirA(R118G)-YFP-NLS
Plasmid#127365PurposeBinary vector for expressing nuclear BirA* (R118G)-YFP under the UBQ10 promoter in plantsDepositorInsertBirA* (R118G mutant)
UseTagsGS linker, NLS, V5, and YFPExpressionPlantMutationR118GPromoterArabidopsis UBQ10 promoterAvailable sinceSept. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-GFP (F64L, S65T, V163A)-TRP1
Plasmid#53184Purposechromosomal tagging with stable GFPDepositorInsertTRP1 (TRP1 Budding Yeast)
UseYeast genomic targetingTagsGFP (F64L, S65T, V163A)ExpressionMutationPromoterAvailable sinceMay 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
pIhh742bp-Luc
Plasmid#53605PurposeReporter construct containing a 742 bp promoter region of the Ihh gene in front of a luciferase gene for in vitro DNA transfection assaysDepositorInsertIhh promoter
UseLuciferase; ReporterTagsExpressionMutationPromoterAvailable sinceJune 30, 2014AvailabilityAcademic Institutions and Nonprofits only -
pRK-flag-USP19 1-493
Plasmid#78581PurposeExpression flag-USP19 1-493 in mammalian cellsDepositorInsertflag-USP19 1-493 (USP19 Human)
UseTagsflagExpressionMammalianMutationmutated 494 residue to stop codonPromoterCMVAvailable sinceJune 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRK-flag-USP19 494-1318
Plasmid#78587PurposeExpression flag-USP19 494-1318 in mammalian cellsDepositorInsertflag-USP19 494-1318 (USP19 Human)
UseTagsflagExpressionMammalianMutationdeleted amino acid 1-493PromoterCMVAvailable sinceJune 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
2674 pBS Bcl-2 P1+P2
Plasmid#8746DepositorInsertBcl-2 P1+P2 (BCL2 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 6, 2005AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-kcnj11
Plasmid#173139PurposeThis gene was cloned into the gateway middle entry vector. It can be used for generating antisense riboprobe or gene expression using a gateway system.DepositorInsertkcnj11 (kcnj11 Zebrafish)
UsePcr cloning vectorTagsExpressionMutationPromoterNo promoter at 5 end; T7 promoter at 3 end.Available sinceSept. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAH35 (Pmyo-2::cGAL-N::let-858 3'UTR)
Plasmid#107134PurposecGAL-N driver under the control of C. elegans myo-2 promoterDepositorInsertNLS::cGAL(DBD)::gp41-1-N-intein
UseTagsExpressionWormMutationPromoterC. elegans myo-2 promoterAvailable sinceMarch 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pIhh-5A9-LUC
Plasmid#46318DepositorInsert5 copies of A9 sequence from Ihh promoter
UseTagsluciferaseExpressionMammalianMutationPromotermouse osteocalcin gene 2 promoterAvailable sinceAug. 2, 2013AvailabilityAcademic Institutions and Nonprofits only -
His10-B1 Integrin
Plasmid#186139PurposeFor expression of recombinant his10-beta1 integrin cytosolic domain in bacteriaDepositorInsertITGB1 (ITGB1 Human)
UseTagsMBP + uncleavable His10ExpressionBacterialMutationPromoterLacAvailable sinceJune 30, 2022AvailabilityAcademic Institutions and Nonprofits only