We narrowed to 5,576 results for: pCas

1. A3A-PBE-ΔUGI

   Plasmid

   #119770

   Purpose

   Conversion of C to T in wheat plants or monocotyledons protoplasts without uracil glycosylase inhibitor (UGI)

   Depositor

   Caixia Gao

   Article

   Zong et al Nat Biotechnol. 2018 Oct 1. pii: nbt.426

   Insert

   APOBEC3A-nCas9-ΔUGI-NLS
   

   Use

   Tags

   NLS

   Expression

   Plant

   Mutation

   nCas9 has a mutation D10A in wild type SpCas9

   Promoter

   maize Ubiquitin-1

   Available since

   Dec. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJT42_GalL_BE-PAPAPA

   Plasmid

   #145035

   Purpose

   Expresses BE-PAPAPA in yeast cells

   Depositor

   Ralph Bock

   Article

   Tan et al Nat Commun. 2019 Jan 25;10(1):439. doi:

   Insert

   BE-PAPAP
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   spCas9(D10A)

   Promoter

   GalL

   Available since

   July 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAtCas9-D10A_2

   Plasmid

   #91160

   Purpose

   Cas9 only expression, Cas9 Type: AtCas9_D10A (nickase), Plant Selection: 2x35S:npt II

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Insert

   AtCas9_D10A (nickase)
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   D10A

   Promoter

   Available since

   July 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. L40C-CRISPR.EFS.PAC

   Plasmid

   #89393

   Purpose

   Lentiviral CRISPR-Cas9 delivery for SpCas9 and sgRNA (hU6), PAC (Puromycin resistance) coexpression, EFS Promoter driven

   Depositor

   Dirk Heckl

   Article

   Reimer et al Haematologica. 2017 Jun 1. pii: haematol

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET42b-A3A-PBE-ΔUGI

   Plasmid

   #119771

   Purpose

   To express the recombinant APOBEC3A in vitro

   Depositor

   Caixia Gao

   Article

   Zong et al Nat Biotechnol. 2018 Oct 1. pii: nbt.426

   Insert

   APOBEC3A-nCas9-NLS
   

   Use

   Tags

   6*HIS and NLS

   Expression

   Bacterial

   Mutation

   nCas9 has a mutation D10A in wild type SpCas9

   Promoter

   T7 promoter

   Available since

   June 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCFL-mPML-3KRmut-iB

   Plasmid

   #140287

   Purpose

   Expression of mouse PML KR mutant

   Depositor

   Yusuke Miyanari

   Article

   Kurihara et al Mol Cell. 2020 May 7;78(3):493-505.e8. d

   Insert

   PML (Pml Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   May 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. px330-gp78

   Plasmid

   #61833

   Purpose

   to make gp78 Knockout cell line

   Depositor

   Yihong Ye

   Article

   Liu et al Elife. 2014;3:e01369. doi: 10.7554/eLife

   Insert

   gp78 (AMFR Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   Feb. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

8. pAYC

   Plasmid

   #62657

   Purpose

   Mammalian expression of iCre recombinase from the broadly active CAG promoter/enhancer

   Depositor

   Masato Ohtsuka

   Article

   Ohtsuka et al BMC Genomics. 2015 Apr 9;16:274. doi: 10

   Insert

   iCre
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 24, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pJT43_GalL_BE-P(AP)3

   Plasmid

   #145036

   Purpose

   Expresses BE-P(AP)3 in yeast cells

   Depositor

   Ralph Bock

   Article

   Tan et al Nat Commun. 2019 Jan 25;10(1):439. doi:

   Insert

   BE-(AP)3
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   spCas9(D10A)

   Promoter

   GalL

   Available since

   July 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLKO1-puro-U6-sgRNA-TGM2

    Plasmid

    #69239

    Purpose

    U6 driven SpCas9 sgRNA expression for TGM2 site

    Depositor

    Scot Wolfe

    Article

    Bolukbasi et al Nat Methods. 2015 Oct 19. doi: 10.1038/n

    Insert

    TGM2 sgRNA (TGM2 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Oct. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAT15485_Hypa-ABE8e

    Plasmid

    #174123

    Purpose

    Expresses ABE8e fused with Hypa-SpCas9

    Depositor

    Ervin Welker

    Article

    Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

    Insert

    Hypa-ABE8e
    

    Use

    CRISPR

    Tags

    Nucleoplasmin NLS and SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    EF1-a

    Available since

    Nov. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. CAGhCBFA2T2

    Plasmid

    #84363

    Purpose

    FLAG-HA tagged human CBFA2T2

    Depositor

    Danny Reinberg

    Article

    Tu et al Nature. 2016 Jun 8;534(7607):387-90. doi

    Insert

    CBFA2T2 (CBFA2T2 Human)
    

    Use

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Feb. 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBI101.1/ProGOXL1:GUS

    Plasmid

    #175557

    Purpose

    Binary vector for Agrobacterium-mediated plant transformation - Promoter:GUS reporter for expression analysis of GOXL genes

    Depositor

    George Haughn

    Article

    Sola et al Plant Cell Physiol. 2021 May 27. pii: 62

    Insert

    ProGOXL1
    

    Use

    Tags

    GUS

    Expression

    Plant

    Mutation

    Promoter

    GOXL1

    Available since

    Jan. 13, 2022

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. pCFL-mPML-2CAMut-iB

    Plasmid

    #140286

    Purpose

    Expression of mouse PML Ca mutant

    Depositor

    Yusuke Miyanari

    Article

    Kurihara et al Mol Cell. 2020 May 7;78(3):493-505.e8. d

    Insert

    PML (Pml Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    CA mutant (RING finger mutant)

    Promoter

    CAG

    Available since

    May 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBZ191-pU6-sgBFP-CBh-sv40NLS-Cas9-NLS-T2A-mCherry

    Plasmid

    #170183

    Purpose

    Expression vector for a sgRNA against BFP and spCas9 linked to mCherry via a T2A peptide

    Depositor

    Hunter Fraser

    Article

    Zhao et al CRISPR J. 2022 Jan 24. doi: 10.1089/cris

    Insert

    sgBFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    Jan. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. gRNA-HEK3-Puro

    Plasmid

    #136282

    Purpose

    Expresses a guide RNA targeting HEK3 site

    Depositor

    Zhaohui Ye

    Article

    McGrath et al Nat Commun. 2019 Nov 25;10(1):5353. doi:

    Insert

    sgRNA-HEK3
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Jan. 31, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pTaCas9_6

    Plasmid

    #91169

    Purpose

    Cas9 only expression, Cas9 Type: TaCas9, Plant Selection: PvUbi2:bar

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    TaCas9
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    July 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. CP1041-ABE8e-dimer

    Plasmid

    #138499

    Purpose

    A-to-G base editor

    Depositor

    David Liu

    Article

    Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

    Insert

    ecTadA(WT)-ecTadA(8e)-nSpCas9 (CP1041)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. px330-UFSP2 sgRNA2

    Plasmid

    #134637

    Purpose

    contains sgRNA targeting human UFSP2 for gene knockout

    Depositor

    Yihong Ye

    Article

    Wang et al Cell Res. 2020 Jan;30(1):5-20. doi: 10.1

    Insert

    UFSP2 sgRNA2 (UFSP2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pC1300_pUB10_pco-nCASphi_E9t_V2_U6_AtPDS3_gRNA10

    Plasmid

    #197965

    Purpose

    T-DNA binary vector to express pco-nCasphi driven by UBQ10 gene promoter, and the AtPDS3 guide RNA 10 driven by U6 promoter.

    Depositor

    Steven Jacobsen

    Article

    Li et al Proc Natl Acad Sci U S A. 2023 Jan 24;12

    Inserts

    pco-nCasphi-2
    AtPDS3 gRNA10
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    AtU6-26 and pUBQ10

    Available since

    May 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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