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Plasmid#181723PurposeGalactose inducible expression of Alpha-synuclein-E46KDepositorInsertAlpha-synuclein (SNCA Human)
UseTagsExpressionYeastMutationE46KPromoterAvailable sinceJuly 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAG426-GAL-Alpha-synuclein-A53T
Plasmid#181722PurposeGalactose inducible expression of Alpha-synuclein-A53TDepositorInsertAlpha-synuclein (SNCA Human)
UseTagsExpressionYeastMutationA53TPromoterAvailable sinceJuly 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAG426-GAL-ATXN1-Q85
Plasmid#181712PurposeGalactose inducible expression of ATXN1-Q85DepositorInsertATXN1-Q85 (ATXN1 Human)
UseTagsExpressionYeastMutationextended repeat of 85 Gln residues replacing nati…PromoterAvailable sinceJuly 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUC18T-mini-Tn7T-hph-dCas9
Plasmid#218371PurposeExpression of dCas9 for CRISPR-interferenceDepositorInsertdCas9
UseCRISPRTagsExpressionBacterialMutationPromoterPtetAAvailable sinceJuly 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUC18T-mini-Tn7T-hph-Ptac-VSVG
Plasmid#218365PurposeEmpty backbone for Tn7-site insertion with lacIq-Ptac expression system and C-terminal VSVG fusionsDepositorTypeEmpty backboneUseTagsVSVGExpressionBacterialMutationPromoterAvailable sinceJune 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
nCas9-NG_P2A-mCherry
Plasmid#219934PurposeMammalian expression of Sp-nCas9-NG with P2A-mCherry marker for use with MOBE systemsDepositorInsertSp-nCas9(D10A)-NG_P2A-mCherry
UseCRISPRTagsSV40 bpNLS and SV40 bpNLS-P2A-mCherryExpressionMammalianMutationnCas9(D10A)PromoterCMVAvailable sinceJune 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEL682
Plasmid#205591PurposePE2 for prime editing in riceDepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoterZmUbiAvailable sinceMay 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEL683
Plasmid#205593PurposeCas9-PE2 for prime editing in riceDepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoterZmUbiAvailable sinceMay 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1-BBS18
Plasmid#218718PurposeExpresses N-terminally EGFP-tagged BBS18 in mammalian cellsDepositorInsertBBS18
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceMay 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pWH1266-Apra-sgRNA
Plasmid#218372PurposesgRNA expression plasmid for CRISPR-intereferenceDepositorInsertsgRNA expression cassette
UseCRISPRTagsExpressionBacterialMutationPromoterJ23119Available sinceMay 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAG416-GAL-FUS-R521C
Plasmid#181718PurposeGalactose inducible expression of FUS-R521CDepositorInsertFUS (FUS Human)
UseTagsExpressionYeastMutationR521CPromoterAvailable sinceApril 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAG416-GAL-FUS-P431L
Plasmid#181717PurposeGalactose inducible expression of FUS-P431LDepositorInsertFUS (FUS Human)
UseTagsExpressionYeastMutationP431LPromoterAvailable sinceApril 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAG416-GAL-FUS
Plasmid#181711PurposeGalactose inducible expression of FUSDepositorInsertFUS (FUS Human)
UseTagsExpressionYeastMutationPromoterAvailable sinceApril 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pzk488-pmax-NES-PUFc-FseI[ADAR2-DD-splitN]
Plasmid#196841PurposeExpress NES-PUFc-FseI[ADAR2-DD-splitN]DepositorInsertNES-PUFc-FseI[ADAR2-DD-splitN]
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pzk491-pmax-NES-PUFc-FseI[ADAR2-DD-splitC]
Plasmid#196842PurposeExpress NES-PUFc-FseI[ADAR2-DD-splitC]DepositorInsertNES-PUFc-FseI[ADAR2-DD-splitC]
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pzk228-pmax-SgrAI[3xFLAG]-AscI-dPspCas13b(AAAA)
Plasmid#196827PurposeExpress dpdpCas13b with AAAA mutationDepositorInsertdpspCas13b(AAAA)
UseTags3XFLAGExpressionMammalianMutationK367A, D369A and K370APromoterAvailable sinceMarch 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pzk408-pmax-3xflag-dpspCas13b(AAAA)-NES
Plasmid#196828PurposeExpress dpdpCas13b with AAAA mutationDepositorInsertdpspCas13b(AAAA)
UseTags3XFLAG and NESExpressionMammalianMutationK367A, D369A and K370APromoterAvailable sinceMarch 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
6xABRE_A
Plasmid#185803PurposeA synthetic ABA signaling reporter designed by multimerization of ABRE (7bp) and its flanking sequences from the ABI1 promoterDepositorInsert6xABRE(ABI1)+-86MP+erGFP
UseTagsExpressionPlantMutationPromoterAvailable sinceFeb. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHB1
Plasmid#204668PurposepUC19 containing the LL-FRT-erm-FRT-spacer sequence which serves as template for BarSeq deletion constructsDepositorInsertFRT-erm-FRT
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
RPO41 deltaN100
Plasmid#205073PurposeRPO41 protein expressionDepositorInsertRPO41 (RPO41 Budding Yeast)
UseTagsExpressionBacterialMutationLacks N Terminal 100 amino acidsPromoterAvailable sinceFeb. 2, 2024AvailabilityAcademic Institutions and Nonprofits only