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Showing: 4661 - 4680 of 46098 results

  5. Δ1-60MEK1C222

    Plasmid
    #164641
    Purpose
    Bacterial expression plasmid for His6-ΔMEK1C222
    Depositor
    Ben Davis
    Article
    Lai et al Cell. 2021 Apr 27. pii: S0092-8674(21)00
    Insert
    N-terminal truncated mitogen-activated protein kinase kinase 1 (MAP2K1 Human)
    Use
    Tags
    His6-tag
    Expression
    Bacterial
    Mutation
    S222C/C277S/C376S; residues 1-60 at N-terminus we…
    Promoter
    T7
    Available since
    May 5, 2021
    Availability
    Academic Institutions and Nonprofits only
    164641-plasmid-map-sequence-id-328776

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  6. Δ1-60MEK1C218

    Plasmid
    #164643
    Purpose
    Bacterial expression plasmid for His6-ΔMEK1C218
    Depositor
    Ben Davis
    Article
    Lai et al Cell. 2021 Apr 27. pii: S0092-8674(21)00
    Insert
    N-terminal truncated mitogen-activated protein kinase kinase 1 (MAP2K1 Human)
    Use
    Tags
    His6-tag
    Expression
    Bacterial
    Mutation
    S218C/C277S/C376S; residues 1-60 at N-terminus we…
    Promoter
    T7
    Available since
    May 5, 2021
    Availability
    Academic Institutions and Nonprofits only
    164643-plasmid-map-sequence-id-329215

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  7. Δ1-60MEK1C218C222

    Plasmid
    #164645
    Purpose
    Bacterial expression plasmid for His6-ΔMEK1C218C222
    Depositor
    Ben Davis
    Article
    Lai et al Cell. 2021 Apr 27. pii: S0092-8674(21)00
    Insert
    N-terminal truncated mitogen-activated protein kinase kinase 1 (MAP2K1 Human)
    Use
    Tags
    His6-tag
    Expression
    Bacterial
    Mutation
    S218C/S222C/C277S/C376S; residues 1-60 at N-termi…
    Promoter
    T7
    Available since
    May 5, 2021
    Availability
    Academic Institutions and Nonprofits only
    164645-plasmid-map-sequence-id-329217

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  16. pC0054-CMV-dPspCas13b-longlinker-ADAR2DD(E488Q/T375G)

    Plasmid
    #103870
    Purpose
    High specificity version of dPspCas13b-ADAR2DD(E488Q) fusions. T375G mutation in the ADAR deaminase domain confers increased specificity with slightly reduced activity.
    Depositor
    Feng Zhang
    Article
    Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi
    Inserts
    PspCas13b
    huADAR2DD (ADARB1 Human)
    Use
    CRISPR
    Tags
    GSGGGGS and HIV NES
    Expression
    Mammalian
    Mutation
    Changed histidne 133 to alanine and histidine 105…
    Promoter
    Available since
    Dec. 13, 2017
    Availability
    Academic Institutions and Nonprofits only
    103870-plasmid-map-sequence-id-199178

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  19. pT3-EF1A-MYC-IRES-lucOS

    Plasmid
    #129776
    Purpose
    Transposon based vector that expresses human MYC followed by IRES and firefly luciferase and the antigens SIYRYYGL (SIY), SIINFEKL (SIN, OVA257-264), and OVA323-339
    Depositor
    Amaia Lujambio
    Article
    Ruiz de Galarreta et al Cancer Discov. 2019 Jun 11. pii: 2159-82
    Insert
    human MYC (MYC Human)
    Use
    Tags
    OS: SIYRYYGL (SIY), SIINFEKL (SIN, OVA257-264), a…
    Expression
    Mammalian
    Mutation
    Promoter
    EF1A
    Available since
    Feb. 13, 2020
    Availability
    Academic Institutions and Nonprofits only
    129776-plasmid-map-sequence-id-263368

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  20. AAV-pCbh-ABE8e-nSpCas9(D10A/A61R)[N-term] (CA19)

    Plasmid
    #197510
    Purpose
    Cbh promoter expression plasmid for N-terminal intein-split AAV construct with N-term of ABE8e-nSpCas9(D10A/A61R) (to be used with SpRY C-term constructs)
    Depositor
    Benjamin Kleinstiver
    Article
    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038
    Insert
    AAV-[ITR]-pCBh-BPNLS-TadA8e-nSpCas9(D10A/A61R)[N-term]-NpuN-BPNLS-[ITR]
    Use
    AAV and CRISPR
    Tags
    BPNLS and NpuN(intein)-BPNLS
    Expression
    Mutation
    ABE8e mutations in TadA and nSpRY(D10A/A61R)
    Promoter
    Cbh
    Available since
    March 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    197510-plasmid-map-sequence-id-386692

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Showing: 4661 - 4680 of 46098 results