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Plasmid#68172PurposeProvides the Arabidopsis thaliana heat shock protein 70B promoter as a level 0 GoldenBraid partDepositorInsertAtHsp70B promoter
UseSynthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceOct. 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-His3MX6-PGAL1-3HA
Plasmid#41610DepositorInsertGAL1 promoter (GAL1 Budding Yeast)
UseYeast genomic targetingTags3xHA, A. gossypii translation elongation factor 1…ExpressionMutationPromoterAvailable sinceDec. 10, 2012AvailabilityAcademic Institutions and Nonprofits only -
pSEM228 - [Pmlc-1 | mNeonGreen(2x NLS) | cbr-tbb-2 UTR]
Plasmid#159895PurposeFluorescent co-injection marker to identify extrachromosomal arrays in C. elegansDepositorInsertPmlc-1 | mNeonGreen(2x NLS) | cbr-tbb-2 UTR
UseTagsExpressionWormMutationPromoterAvailable sinceOct. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRS-Dhh1-PA(D195A,E196A,S226A,T228A)(405)
Plasmid#122067PurposeExpressing Dhh1-PADepositorInsertDhh1-PA (DHH1 Budding Yeast)
UseTagsPAExpressionYeastMutationAspartic acid 195 to alanine; Glutamic acid 196 t…PromoterAvailable sinceApril 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pWB-Flag-IKKe
Plasmid#44115DepositorInsertIKKe (IKBKE Human)
UseRetroviralTagsFlagExpressionMutationPromoterAvailable sinceMarch 25, 2013AvailabilityAcademic Institutions and Nonprofits only -
pSKA397
Plasmid#80380Purposemini-Tn7 delivery plasmid with metE under the cpcG2Δ59 promoter.DepositorInsertscat
metE
UseSynthetic Biology; Mini-tn7 delivery vectorTagsExpressionBacterialMutationPromotercat promoter and cpcG2Δ59Available sinceOct. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
PRE-Hsp70BbCas9_1.3
Plasmid#190797Purposeaka PRE-Hsp70BbCas9 or 2gypsyPRE-Hsp70Bb-Cas9-T2A-eGFP; heat-shock inducible Cas9-T2A-eGFP expression regulated by upstream Polycomb response elements with two gypsy insulator elements. Final productDepositorInsert2gypsyPRE-Hsp70Bb-Cas9-T2A-eGFP
UseTagsExpressionInsectMutationPromoterAvailable sinceNov. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK-2-Zfp36-3'UTR
Plasmid#61780PurposeLuciferase reporter containing the 3'UTR of mouse Zfp36DepositorInsertmouse Zfp36 3'UTR
UseLuciferaseTagsExpressionMutationPromoterAvailable sinceMay 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
Flag-Braf R188L
Plasmid#118338PurposeExpression of human BrafDepositorInsertBraf R188L (BRAF Human)
UseTagsFlagExpressionMammalianMutationR188LPromoterCMVAvailable sinceNov. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDUAL_2_T5
Plasmid#155175Purposesee pDUAL_T5: exchanged origin of replication (pBR322) and introduced bom siteDepositorInsertsSucrose Phosphorylase from Bifidobacterium adolescentis (BAD_RS00415 Bifidobacterium adolescentis)
Cellobiose phosphorylase from Cellulomonas uda
UseSynthetic BiologyTagsHis Tag and Strep - Tag IIExpressionBacterialMutationPromoterAvailable sinceSept. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
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pSUPER.retro-{beta}4 SCR
Plasmid#16267DepositorInsertSCR Beta 4 Integrin (ITGB4 Human)
UseRNAi and RetroviralTagsExpressionMammalianMutationPromoterAvailable sinceMarch 6, 2008AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+) neo with Smad5 CDS
Plasmid#61800PurposepcDNA3.1(+) with full length mouse Smad5 CDSDepositorInsertSmad5 CDS (Smad5 Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pExT
Plasmid#36889DepositorInsertsT (i.e., ATG, start codon as a first exon)
GFP
beta-globin intron
Hygro
UseTagsExpressionMammalianMutationdeleted nucleotides before nucleotide 275 and ins…PromoterCAG (chicken beta actin promoter and CMV enhancer…Available sinceAug. 8, 2012AvailabilityAcademic Institutions and Nonprofits only -
pExG
Plasmid#36888DepositorInsertsGFP
tdTomato-3Myc
beta-globin intron
Hygro
UseTags3 Myc tagsExpressionMammalianMutationdeleted nucleotides after nucleotide 274, inserti…PromoterCAG (chicken beta actin promoter and CMV enhancer…Available sinceAug. 17, 2012AvailabilityAcademic Institutions and Nonprofits only -
Ef1a-QCP-3xBFP
Plasmid#158206PurposeQb coat protein fused to three repeats of the blue fluorescent protein under a Efla promoter.DepositorInsertQCP under the Elfa promoter and three BFP tags
UseSynthetic BiologyTags3xBFPExpressionMammalianMutationPromoterElfaAvailable sinceMarch 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBIND-HBSP
Plasmid#53113PurposeExpression of GAL4BD-tagged Hepatitis B splicing proteinDepositorInsertHBSP
UseTagsGAL4BDExpressionMammalianMutationPromoterCMVAvailable sinceMay 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-flag-UTR
Plasmid#183234PurposeMammalian expression empty backboneDepositorTypeEmpty backboneUseTagsFLAG tagExpressionMammalianMutationPromoterCMVAvailable sinceOct. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pVRb11_3726
Plasmid#49707DepositorInsertsfgfp
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPecf11_3726Available sinceJan. 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBSU6-shTim23/CMV-eGFP
Plasmid#89795PurposeExpresses shRNA against TIM23 with a separate promoter for expression of GFP.DepositorInsertshRNA Tim23 (Timm23 Mouse)
UseTagseGFP under separate promoterExpressionMammalianMutationPromoterU6Available sinceMay 31, 2017AvailabilityAcademic Institutions and Nonprofits only