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pX330_NANOS3_cterm

Plasmid

#222904

Purpose

Cas9/sgRNA plasmid for targeting NANOS3

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2022.07.11.499564

Insert

Cas9, NANOS3 sgRNA (NANOS3 Synthetic, Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

July 22, 2024

Availability

Academic Institutions and Nonprofits only

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pPB-cT3G-cERP2-FIGLA

Plasmid

#222929

Purpose

PiggyBac transposon plasmid for doxycycline inducible expression of FIGLA

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2022.07.11.499564

Insert

FIGLA (FIGLA Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 22, 2024

Availability

Academic Institutions and Nonprofits only

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pPB-cT3G-cERP2-HHEX

Plasmid

#222928

Purpose

PiggyBac transposon plasmid for doxycycline inducible expression of HHEX

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2022.07.11.499564

Insert

HHEX (HHEX Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 22, 2024

Availability

Academic Institutions and Nonprofits only

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pPB-cT3G-cERP2-DLX5

Plasmid

#222927

Purpose

PiggyBac transposon plasmid for doxycycline inducible expression of DLX5

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2022.07.11.499564

Insert

DLX5 (DLX5 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 19, 2024

Availability

Academic Institutions and Nonprofits only

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pPB-cT3G-cERH-LHX8

Plasmid

#222921

Purpose

PiggyBac transposon plasmid for doxycycline inducible expression of LHX8

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2022.07.11.499564

Insert

LHX8 (LHX8 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 18, 2024

Availability

Academic Institutions and Nonprofits only

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pCDNA3-TEX264-SIM2_mut-cFlag

Plasmid

#220206

Purpose

Mammalian expression of TEX264 with SIM2 motif mutated, C-terminal Flag

Depositor

Kristijan Ramadan

Article

Fielden et al Nat Commun. 2020 Mar 9;11(1):1274. doi:

Insert

TEX264 (TEX264 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

p.111ILSIW>ALAIA; c.421A>G,422T>C,427T&g…

Promoter

CMV

Available since

June 12, 2024

Availability

Academic Institutions and Nonprofits only

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pCDNA3-TEX264-SHP1mut-cFlag

Plasmid

#220208

Purpose

Mammalian expression of TEX264 with SHP domain mutated, C-terminal Flag

Depositor

Kristijan Ramadan

Article

Fielden et al Nat Commun. 2020 Mar 9;11(1):1274. doi:

Insert

TEX264 (TEX264 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

p.280GEGPL>RERPA; c.838G>C,844G>A,850T&g…

Promoter

CMV

Available since

June 12, 2024

Availability

Academic Institutions and Nonprofits only

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pCDNA5-TEX264-deltaLRR-cSSH

Plasmid

#220210

Purpose

Mammalian expression of TEX264, 2-25 residues (LRR) truncated, C-terminal twin-Strep_HA tag

Depositor

Kristijan Ramadan

Article

Fielden et al Nat Commun. 2020 Mar 9;11(1):1274. doi:

Insert

TEX264 (TEX264 Human)

Use

Tags

Strep-strep-HA

Expression

Mammalian

Mutation

c.4-75del / p.2-24del

Promoter

CMV/TetO

Available since

June 12, 2024

Availability

Academic Institutions and Nonprofits only

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pCDNA5-TEX264-SIM2_mut-cGFP

Plasmid

#220213

Purpose

Mammalian expression of TEX264 with SIM2 motif mutated, fused at C-terminus to GFP

Depositor

Kristijan Ramadan

Article

Fielden et al Nat Commun. 2020 Mar 9;11(1):1274. doi:

Insert

TEX264 (TEX264 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

p.111ILSIW>ALAIA; c.421A>G,422T>C,427T&g…

Promoter

CMV/TetO

Available since

June 12, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_V5-TWIST1_NEUROD1loop

Plasmid

#215032

Purpose

Mammalian expression of human TWIST1 (with NEUROD1 loop)

Depositor

Joanna Wysocka

Article

Kim et al Cell. 2024 Feb 1;187(3):692-711.e26. doi

Insert

twist family bHLH transcription factor 1 (TWIST1 Human)

Use

Tags

V5

Expression

Mammalian

Mutation

TWIST1 bHLH loop (aa134-141, IIPTLPSD) replaced w…

Promoter

CMV

Available since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_V5-TWIST1_NEUROG2loop

Plasmid

#215033

Purpose

Mammalian expression of human TWIST1 (with NEUROG2 loop)

Depositor

Joanna Wysocka

Article

Kim et al Cell. 2024 Feb 1;187(3):692-711.e26. doi

Insert

twist family bHLH transcription factor 1 (TWIST1 Human)

Use

Tags

V5

Expression

Mammalian

Mutation

TWIST1 bHLH loop (aa137-141, TLPSD) replaced with…

Promoter

CMV

Available since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_V5-TWIST1_HAND2loop

Plasmid

#215034

Purpose

Mammalian expression of human TWIST1 (with HAND2 loop)

Depositor

Joanna Wysocka

Article

Kim et al Cell. 2024 Feb 1;187(3):692-711.e26. doi

Insert

twist family bHLH transcription factor 1 (TWIST1 Human)

Use

Tags

V5

Expression

Mammalian

Mutation

TWIST1 bHLH loop (aa137-141, TLPSD) replaced with…

Promoter

CMV

Available since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_V5-TWIST1_TAL1loop

Plasmid

#215035

Purpose

Mammalian expression of human TWIST1 (with TAL1 loop)

Depositor

Joanna Wysocka

Article

Kim et al Cell. 2024 Feb 1;187(3):692-711.e26. doi

Insert

twist family bHLH transcription factor 1 (TWIST1 Human)

Use

Tags

V5

Expression

Mammalian

Mutation

TWIST1 bHLH loop (aa137-141, TLPSD) replaced with…

Promoter

CMV

Available since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_V5-TWIST1_NEUROD1L

Plasmid

#215043

Purpose

Mammalian expression of human TWIST1 (with fragment of loop from NEUROD1)

Depositor

Joanna Wysocka

Article

Kim et al Cell. 2024 Feb 1;187(3):692-711.e26. doi

Insert

twist family bHLH transcription factor 1 (TWIST1 Human)

Use

Tags

V5

Expression

Mammalian

Mutation

Left portion of TWIST1 bHLH loop (aa137-139, TLP)…

Promoter

CMV

Available since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_V5-TWIST1_NEUROD1R

Plasmid

#215044

Purpose

Mammalian expression of human TWIST1 (with fragment of loop from NEUROD1)

Depositor

Joanna Wysocka

Article

Kim et al Cell. 2024 Feb 1;187(3):692-711.e26. doi

Insert

twist family bHLH transcription factor 1 (TWIST1 Human)

Use

Tags

V5

Expression

Mammalian

Mutation

Right portion of TWIST1 bHLH loop (aa139-141, PSD…

Promoter

CMV

Available since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_V5-TWIST1_NEUROD1M

Plasmid

#215045

Purpose

Mammalian expression of human TWIST1 (with fragment of loop from NEUROD1)

Depositor

Joanna Wysocka

Article

Kim et al Cell. 2024 Feb 1;187(3):692-711.e26. doi

Insert

twist family bHLH transcription factor 1 (TWIST1 Human)

Use

Tags

V5

Expression

Mammalian

Mutation

Middle portion of TWIST1 bHLH loop (aa139, P) rep…

Promoter

CMV

Available since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV_FKBP-V5-ALX1

Plasmid

#215023

Purpose

AAV vector for knocking in an N-terminal tag (FKBP12F36V and V5) for human ALX1

Depositor

Joanna Wysocka

Article

Kim et al Cell. 2024 Feb 1;187(3):692-711.e26. doi

Insert

ALX homeobox 1 (ALX1 Human)

Use

AAV

Tags

FKBP-V5

Expression

Mutation

Promoter

Available since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

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pGL3-noSV40-humanEC1.45_min1-2_4XEboxMutant

Plasmid

#215026

Purpose

Firefly luciferase enhancer reporter plasmid with minimal enhancer regions 1 and 2 (min1 and min2) from SOX9 enhancer cluster EC1.45 with E-boxes mutated

Depositor

Joanna Wysocka

Article

Kim et al Cell. 2024 Feb 1;187(3):692-711.e26. doi

Insert

Minimal enhancer regions 1 and 2 (min1 and min2) from SOX9 enhancer cluster EC1.45 with E-boxes mutated

Use

Luciferase

Tags

Expression

Mutation

4X E-box mutations within Coordinator motifs

Promoter

SV40

Available since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti-CMV-GFP-Puro_TAL1-short

Plasmid

#210641

Purpose

Overexpression of TAL1-short

Depositor

Maayan Salton

Article

Sharma et al PLoS Biol. 2023 Jun 28;21(6):e3002175. d

Insert

TAL1-short (TAL1 Human)

Use

Lentiviral

Tags

GFP

Expression

Mutation

Promoter

Available since

Jan. 3, 2024

Availability

Academic Institutions and Nonprofits only

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Tet-pLKO-puro-shTAL1

Plasmid

#210640

Purpose

Knockdown of TAL1 endogenous (3'UTR)

Depositor

Maayan Salton

Article

Sharma et al PLoS Biol. 2023 Jun 28;21(6):e3002175. d

Insert

TAL1 3' UTR gRNA (TAL1 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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