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Showing: 4621 - 4640 of 5244 results

  6. AXBE

    Plasmid
    #204605
    Purpose
    Mammalian expression of adenine transversion base editor AXBE
    Depositor
    Dali Li
    Article
    Chen et al Nat Biotechnol. 2023 Jun 15. doi: 10.103
    Insert
    bpNLS-TadA-8e-nSpCas9-mAAG-bpNLS-P2A-GFP
    Use
    CRISPR
    Tags
    6*His
    Expression
    Mammalian
    Mutation
    S. pyogenes Cas9 (D10A)
    Promoter
    Available since
    Aug. 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    204605-plasmid-map-sequence-id-404718

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  8. Sniper-Cas9

    Plasmid
    #138559
    Purpose
    Expresses human codon-optimized Sniper-SpCas9 and blasticidin resistance: EFS promoter-Sniper-Cas9-NLS-FLAG-P2A-BSD
    Depositor
    Hyongbum Kim
    Article
    Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133
    Insert
    Sniper-Cas9
    Use
    CRISPR and Lentiviral
    Tags
    BSD, FLAG, and NLS
    Expression
    Mammalian
    Mutation
    F539S, M763I, K890N
    Promoter
    EFS
    Available since
    June 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    138559-plasmid-map-sequence-id-298901

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  12. pUC57-Amp_CRISPIE donor B5

    Plasmid
    #172846
    Purpose
    CRISPIE donor B5 (Zhong et al, eLife 2021), CDS of VCL exon21-mEGFP, translational phase (0-stop), excised by ACTB i4 sgRNA or DRS-1 sgRNA (with SpCas9).
    Depositor
    Haining Zhong
    Article
    Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1
    Insert
    CRISPIE designer exon (phase 0-stop) encoding the CDS of VCL exon 21 fused to mEGFP
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    172846-plasmid-map-sequence-id-340979

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  13. FKBP*-PTEN

    Plasmid
    #86781
    Purpose
    Fusion of PTEN with a modified human FKBP variant harboring the F36V and L106P point mutations (FKBP*) leads to degradation of the fusion protein.
    Depositor
    Britta Eickholt
    Article
    An et al PLoS One. 2015 Dec 30;10(12):e0145783. d
    Insert
    PTEN (PTEN Human)
    Use
    Tags
    FKBP* (F36V and L106P) and linker
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 6, 2017
    Availability
    Academic Institutions and Nonprofits only
    86781-plasmid-map-sequence-id-169199

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  19. AAEL006511-Cas9

    Plasmid
    #100580
    Purpose
    The promoter of AAEL006511 drive expression of Streptococcus pyogenes Cas9 gene in Aedes aegypti
    Depositor
    Omar Akbari
    Article
    Li et al Proc Natl Acad Sci U S A. 2017 Nov 14. p
    Inserts
    AAEL006511-hspcas9-GFP
    Opie2-dsRed
    Use
    CRISPR
    Tags
    GFP and dsRED
    Expression
    Insect
    Mutation
    Promoter
    AAEL006511 and Opie2
    Available since
    Oct. 10, 2017
    Availability
    Academic Institutions and Nonprofits only
    100580-plasmid-map-sequence-id-193175

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  20. MSP712

    Plasmid
    #65768
    Purpose
    Bacterial expression plasmid for Sp-dCas9 & sgRNA (need to clone in spacer into BsaI sites): T7-humanSpdCas9(D10A/H840A)-T7-BsaIcassette-Sp-sgRNA
    Depositor
    Keith Joung
    Article
    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature
    Insert
    mammalian codon-optimized Streptococcus pyogenes dCas9 (D10A/H840A)-NLS-3XFlag, and SpCas9 gRNA
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Bacterial
    Mutation
    D10A and H840A mutations in Cas9
    Promoter
    T7 (x2)
    Available since
    June 24, 2015
    Availability
    Academic Institutions and Nonprofits only
    65768-plasmid-map-sequence-id-112655

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Showing: 4621 - 4640 of 5244 results