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Showing: 4621 - 4640 of 150170 results

  1. GST-mDia1(FH1FH2DAD)-His

    Plasmid
    #85822
    Purpose
    contains the formin homolgy domains: FH2 is required for nucleation, processive tracking of barbed-end of actin filaments ; FH1 permits enhancement of actin polymerization rate.
    Depositor
    Guillaume Romet-Lemonne, Antoine Jégou
    Article
    Jegou et al Nat Commun. 2013;4:1883. doi: 10.1038/nc
    Insert
    Protein diaphanous homolog 1 (Diaph1 Mouse)
    Use
    Tags
    8*His and GST
    Expression
    Bacterial
    Mutation
    Promoter
    tac
    Available since
    July 20, 2017
    Availability
    Academic Institutions and Nonprofits only
    85822-plasmid-map-sequence-id-183287

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  7. Gs(short)-CASE

    Plasmid
    #168124
    Purpose
    Encodes a BRET-based activity sensor for the heterotrimeric G protein Gs. Composed of the subunits G alpha s(short isoform) (GNAS) tagged with NanoLuciferase, G beta 1 (GNB1) and Venus-tagged G gamma 1 (GNG1).
    Depositor
    Gunnar Schulte
    Article
    Schihada et al Sci Signal. 2021 Sep 7;14(699):eabf1653.
    Insert
    GNAS, GNB1, GNG1 (GNGT1, GNAS, GNB1 Human)
    Use
    Luciferase
    Tags
    cpVenus on GNG1
    Expression
    Mammalian
    Mutation
    NLuc is inserted at N136/V137 within GNAS
    Promoter
    Available since
    July 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    168124-plasmid-map-sequence-id-332868

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  9. pAAV-S5E2-GCaMP6f (AAV PHP.eB)

    Viral Prep
    #135632-PHPeB
    Purpose
    Ready-to-use AAV PHP.eB particles produced from pAAV-S5E2-GCaMP6f (#135632). In addition to the viral particles, you will also receive purified pAAV-S5E2-GCaMP6f plasmid DNA. Expression of GCaMP6f under the control of the E2 regulatory element. These AAV were produced with the PHPeB serotype, which permits efficient transduction of the central nervous system. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Jordane Dimidschstein
    Article
    Vormstein-Schneider et al Nat Neurosci. 2020 Dec;23(12):1629-1636.
    Promoter
    Tags
    None
    Available since
    Sept. 13, 2021
    Availability
    Academic Institutions and Nonprofits only

  10. pB1H2 UV5 Zif268-cons (2-hybrid bait plasmid)

    Plasmid
    #128164
    Purpose
    UV5 promoter drives expression of Zif268-consensus peptide fusion. Pair with pGHUC w-ERBIN as a positive control (turns on HIS3/GFP).
    Depositor
    Marcus Noyes
    Article
    Ichikawa et al ACS Synth Biol. 2019 May 17;8(5):918-928
    Insert
    10 amino acid glycine/serine linker + ERBIN PDZ consensus ligand (WETWV)
    Use
    Synthetic Biology
    Tags
    FLAG and zif268
    Expression
    Bacterial
    Mutation
    Promoter
    UV5
    Available since
    Jan. 3, 2020
    Availability
    Academic Institutions and Nonprofits only
    128164-plasmid-map-sequence-id-267570

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  14. pGHUC w-ERBIN (2-hybrid prey plasmid)

    Plasmid
    #128163
    Purpose
    UV5 promoter drives expression of omega-erbin fusion. Pair with pB1H2 UV5 Zif268-cons as a positive control (turns on HIS3/GFP).
    Depositor
    Marcus Noyes
    Article
    Ichikawa et al ACS Synth Biol. 2019 May 17;8(5):918-928
    Insert
    ERBIN PDZ domain (ERBIN Synthetic)
    Use
    Synthetic Biology
    Tags
    FLAG and Omega subunit of RNA polymerase
    Expression
    Bacterial
    Mutation
    Promoter
    UV5
    Available since
    Jan. 3, 2020
    Availability
    Academic Institutions and Nonprofits only
    128163-plasmid-map-sequence-id-267568

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  18. pT076

    Plasmid
    #137880
    Purpose
    Donor vector for integration at the human AAVS1 safe harbor locus of Doxicycline-inducible dCas9-VPR-T2A-EGFP
    Depositor
    Lindy Barrett
    Article
    Hazelbaker et al Sci Rep. 2020 Jan 20;10(1):635. doi: 10.
    Insert
    TRE3G-dCas9-VPR-T2A-EGFP-CAG-TetON-NeoR-SA
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    137880-plasmid-map-sequence-id-271983

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  19. pRosetta

    Plasmid
    #59700
    Purpose
    Lentiviral vector for use as a standard for titering experiments when trying to compare vectors with different selection markers or to determine appropriate antibiotic conditions.
    Depositor
    John Doench, David Root
    Inserts
    Puro
    BlastR
    EGFP
    Use
    Lentiviral
    Tags
    2A
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Sept. 3, 2014
    Availability
    Academic Institutions and Nonprofits only
    59700-plasmid-map-sequence-id-140560

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Showing: 4621 - 4640 of 150170 results