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Showing: 4601 - 4620 of 8506 results

  1. pYFP-NEAT1m_v1-RT

    Plasmid
    #97089
    Purpose
    Encodes a HR repair template for knock-in a YFP expression cassette downstream of the poly(A) signal of human NEAT1_1 isoform. Best used with px330-NEAT1m_v1 vector.
    Depositor
    Archa Fox
    Article
    Li et al RNA. 2017 Mar 21. pii: rna.059477.116. d
    Insert
    NEAT1m_v1-RT (NEAT1 Human)
    Use
    CRISPR
    Tags
    EYFP
    Expression
    Mutation
    Promoter
    CMV
    Available since
    July 31, 2017
    Availability
    Academic Institutions and Nonprofits only
    97089-plasmid-map-sequence-id-189689

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  2. pLenti-gRNA-puro

    Plasmid
    #180426
    Purpose
    Expresses puromycin resistance gene and scrambled gRNA for stable integration in mammalian cells; useful as control and template for new gRNAs by mutagenesis.
    Depositor
    Moshe Szyf
    Article
    Sapozhnikov et al Nat Commun. 2021 Sep 29;12(1):5711. doi:
    Insert
    gRNAscr
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    human U6
    Available since
    Feb. 18, 2022
    Availability
    Academic Institutions and Nonprofits only
    180426-plasmid-map-sequence-id-354805

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  4. pYFP-NEAT1_IS_RT

    Plasmid
    #97091
    Purpose
    Encodes a HR repair template that mutates the poly(A) signal as well as a few splicing factor binding sites of human NEAT1_1 isoform. Best used with px335-NEAT1_IS_v1 and v2 vectors.
    Depositor
    Archa Fox
    Article
    Li et al RNA. 2017 Mar 21. pii: rna.059477.116. d
    Insert
    NEAT1_IS_RT (NEAT1 Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    CMV
    Available since
    July 31, 2017
    Availability
    Academic Institutions and Nonprofits only
    97091-plasmid-map-sequence-id-189691

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  5. AICSDP-22:CETN2-mTagRFP-T

    Plasmid
    #101781
    Purpose
    Homology arms and mTagRFPT-linker sequence for N-terminus tagging of human CETN2
    Depositor
    Allen Institute for Cell Science
    Insert
    CETN2 Homology Arms with mTagRFPT-linker (CETN2 Human)
    Use
    CRISPR; Donor template
    Tags
    mTagRFPT-linker
    Expression
    Mammalian
    Mutation
    homology arms contain point mutations to disrupt …
    Promoter
    Available since
    Oct. 31, 2017
    Availability
    Industry, Academic Institutions, and Nonprofits
    101781-plasmid-map-sequence-id-197673

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  6. pRB17

    Plasmid
    #52527
    Purpose
    serves as template for PCR-mediated fusion of U6-promoter with sgRNA
    Depositor
    Klaus Foerstemann
    Article
    Bottcher et al Nucleic Acids Res. 2014 Apr 19.
    Insert
    Drosophila U6C promoter
    Use
    CRISPR; Blunt cloning kit
    Tags
    T7-promoter
    Expression
    Insect
    Mutation
    Promoter
    Available since
    April 14, 2014
    Availability
    Academic Institutions and Nonprofits only
    52527-plasmid-map-sequence-id-136307

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  8. AICSDP-83:EZH2-mEGFP

    Plasmid
    #164499
    Purpose
    Homology arms and mEGFP-linker sequence for N-terminus tagging of human EZH2
    Depositor
    Allen Institute for Cell Science
    Insert
    EZH2 Homology Arms with mEGFP-linker (EZH2 Human)
    Use
    CRISPR; Donor template
    Tags
    mEGFP-linker
    Expression
    Mammalian
    Mutation
    homology arms contain point mutations to disrupt …
    Promoter
    Available since
    Feb. 17, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    164499-plasmid-map-sequence-id-324903

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  9. pUC57-GFP-SFPQ-RT

    Plasmid
    #97090
    Purpose
    Encodes a HR repair template for knock-in of an EGFP insert that fused to the 5'-end of human SFPQ gene, without disturbing its promoter. Best used with px330-GFP-SFPQ vector.
    Depositor
    Archa Fox
    Article
    Li et al RNA. 2017 Mar 21. pii: rna.059477.116. d
    Insert
    EGFP-SFPQ-RT (SFPQ Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    July 31, 2017
    Availability
    Academic Institutions and Nonprofits only
    97090-plasmid-map-sequence-id-189690

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  12. pSUB7

    Plasmid
    #169698
    Purpose
    Template plasmid for generating C-terminal 6-His epitope tag fusions to bacterial genes by "Lambda red" recombineering.
    Depositor
    Nara Figueroa
    Article
    Uzzau et al Proc Natl Acad Sci U S A. 2001 Dec 18;98
    Insert
    6xHis epitope tag adjacent to FRT-flanked aph (KanR) gene
    Use
    Tags
    6xHis tag
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    July 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    169698-plasmid-map-sequence-id-334547

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  14. pExpreS2-1-C3-PA

    Plasmid
    #175446
    Purpose
    HiFi-compatible destination vector for secreted overexpression of 3C-cleavable, C-terminal Protein A tagged proteins with ExpreS2 Platform
    Depositor
    Wyatt Yue
    Article
    Coker et al Front Bioeng Biotechnol. 2022 May 5;10:8
    Type
    Empty backbone
    Use
    Tags
    3C-Protein A and BiP Secretion Peptide
    Expression
    Insect
    Mutation
    Promoter
    Fused Actin-HSP70
    Available since
    March 30, 2022
    Availability
    Academic Institutions and Nonprofits only
    175446-plasmid-map-sequence-id-358138

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  18. pSU315

    Plasmid
    #169697
    Purpose
    Template plasmid for generating C-terminal hemagglutinin (1xHA) epitope tag fusions to bacterial genes by "Lambda red" recombineering.
    Depositor
    Nara Figueroa
    Article
    Uzzau et al Proc Natl Acad Sci U S A. 2001 Dec 18;98
    Insert
    1xHA epitope tag adjacent to FRT-flanked aph (KanR) gene
    Use
    Tags
    1xHA tag
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    July 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    169697-plasmid-map-sequence-id-334518

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  19. pWL502-crRNA

    Plasmid
    #174383
    Purpose
    The plasmids carrying a mini-CRISPR array were used to express crRNAs; article demonstrates use in Haloferax mediterranei
    Depositor
    Jing Han
    Article
    Lin et al Commun Biol. 2021 Aug 25;4(1):1007. doi:
    Insert
    a crRNA targeting the template strand
    Use
    CRISPR; Archaeal expression
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PhaR promoter
    Available since
    Nov. 11, 2021
    Availability
    Academic Institutions and Nonprofits only
    174383-plasmid-map-sequence-id-343840

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  20. pSLC-238

    Plasmid
    #73192
    Purpose
    Template plasmid which encodes kanamycin resistance gene for positive selection and toxin gene (chpB) under the control of rhamnose induceable promoter (PrhaB) for negative selection.
    Depositor
    Swaine Chen
    Article
    Khetrapal et al Nucleic Acids Res. 2015 Jul 27;43(13):e8
    Insert
    PrhaB-chpB (chpB )
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PrhaB
    Available since
    June 30, 2016
    Availability
    Academic Institutions and Nonprofits only
    73192-plasmid-map-sequence-id-151373

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Showing: 4601 - 4620 of 8506 results