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pBS-f1(-)-ACTB-iTagRFP-T

Plasmid

#115916

Purpose

donor to insert iTagRFP-T at the ACTB locus in human cells

Depositor

Erik Sontheimer

Publication

Iyer et al CRISPR J. 2022 Oct;5(5):685-701. doi: 10

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

ACTB-TagRFP-T

Use

Donor template

Tags

TagRFP-T

Available since

March 19, 2021

Availability

Academic Institutions and Nonprofits only

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pAP1680

Plasmid

#137088

Purpose

Plasmid for PCR-generated donor DNA for rapid tagging of proteins with tagRFP

Depositor

Geraldine Seydoux

Publication

Paix et al Curr Protoc Mol Biol. 2019 Dec;129(1):e1

Insert

tagRFP

Use

Pcr template for donor dna

Expression

Bacterial

Available since

March 13, 2020

Availability

Academic Institutions and Nonprofits only

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pJUMP18-pheS_NOC

Plasmid

#127031

Purpose

Basic Part NOC- Complete ORF; PheS; counter-selection marker; mutant phenylalanine tRNA synthetase.

Depositor

Chris French

Publication

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Insert

Part PheS_NOC

Use

Synthetic Biology

Expression

Bacterial

Mutation

pheS A294G promiscuous mutation

Available since

July 1, 2019

Availability

Academic Institutions and Nonprofits only

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pJUMP18-B0034-MV_RN

Plasmid

#127017

Purpose

Basic Part RN- RBS+N-terminus; pET vector Ribosome Binding Site + Met-Val N-terminus start.

Depositor

Chris French

Publication

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Insert

Part B0034m-MV_RN

Use

Synthetic Biology

Expression

Bacterial

Mutation

None

Available since

July 1, 2019

Availability

Academic Institutions and Nonprofits only

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pJUMP18-B0032-MV_RN

Plasmid

#127015

Purpose

Basic Part RN- RBS+N-terminus; B0033m Ribosome Binding Site + Met-Val N-terminus start.

Depositor

Chris French

Publication

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Insert

Part B0032m-MV_RN

Use

Synthetic Biology

Expression

Bacterial

Mutation

None

Available since

July 1, 2019

Availability

Academic Institutions and Nonprofits only

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FB001

Plasmid

#119057

Purpose

Promoter trpC from Aspergillus nidulans domesticated into pUPD2 with GGAG/AATG barcodes. According to FungalBraid/GoldenBraid modular DNA assembly for A. tumefaciens-mediated genetic transformation.

Depositor

Jose Marcos

Publication

Hernanz-Koers et al Fungal Genet Biol. 2018 Jul;116:51-61. d

Insert

Promoter trpC

Use

Synthetic Biology; Domestication of dna parts for…

Available since

March 28, 2019

Availability

Academic Institutions and Nonprofits only

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pDD401

Plasmid

#91828

Purpose

Pmyo-2::GFP FP-slot donor for the SapTrap cloning system

Depositor

Bob Goldstein

Publication

Dickinson et al MicroPubl Biol. 2018 May 1;2018:10.17912

Insert

Pmyo-2::GFP

Use

CRISPR

Expression

Worm

Mutation

Nucleotide 489 of the myo-2 promoter was changed …

Available since

June 23, 2017

Availability

Academic Institutions and Nonprofits only

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pEN_hU6miR-G12-E

Plasmid

#25757

Purpose

Entry vector with human U6 promoter driving mouse G alpha 12 miR30-based shRNA.

Depositor

Iain Fraser

Publication

Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1

Insert

G alpha 12 miR-shRNA (Gna12 Mouse)

Use

Gateway Cloning

Available since

July 14, 2010

Availability

Academic Institutions and Nonprofits only

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SFFV-SOX18-Brd 489

Plasmid

#219383

Purpose

Transcription factor SOX18 with a specific barcode assigned.

Depositor

Filipe Pereira

Publication

Kurochkin et al Cell Syst. 2026 Jan 21;17(1):101457. doi

Insert

SOX18 (SOX18 Human)

Use

Lentiviral

Promoter

The spleen focus-forming virus (SFFV) promoter

Available since

Oct. 29, 2025

Availability

Academic Institutions and Nonprofits only

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SFFV-ZBTB1-Brd 227

Plasmid

#219170

Purpose

Transcription factor ZBTB1 with a specific barcode assigned.

Depositor

Filipe Pereira

Publication

Kurochkin et al Cell Syst. 2026 Jan 21;17(1):101457. doi

Insert

ZBTB1 (ZBTB1 Human)

Use

Lentiviral

Promoter

The spleen focus-forming virus (SFFV) promoter

Available since

Oct. 29, 2025

Availability

Academic Institutions and Nonprofits only

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SFFV-PREB-Brd 239

Plasmid

#219182

Purpose

Transcription factor PREB with a specific barcode assigned.

Depositor

Filipe Pereira

Publication

Kurochkin et al Cell Syst. 2026 Jan 21;17(1):101457. doi

Insert

PREB (PREB Human)

Use

Lentiviral

Promoter

The spleen focus-forming virus (SFFV) promoter

Available since

Oct. 29, 2025

Availability

Academic Institutions and Nonprofits only

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SFFV-ARID3A-Brd 69

Plasmid

#219047

Purpose

Transcription factor ARID3A with a specific barcode assigned.

Depositor

Filipe Pereira

Publication

Kurochkin et al Cell Syst. 2026 Jan 21;17(1):101457. doi

Insert

ARID3A (ARID3A Human)

Use

Lentiviral

Promoter

The spleen focus-forming virus (SFFV) promoter

Available since

Oct. 29, 2025

Availability

Academic Institutions and Nonprofits only

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SFFV-NR4A3-Brd 28

Plasmid

#219010

Purpose

Transcription factor NR4A3 with a specific barcode assigned.

Depositor

Filipe Pereira

Publication

Kurochkin et al Cell Syst. 2026 Jan 21;17(1):101457. doi

Insert

NR4A3 (NR4A3 Human)

Use

Lentiviral

Promoter

The spleen focus-forming virus (SFFV) promoter

Available since

Oct. 29, 2025

Availability

Academic Institutions and Nonprofits only

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SFFV-EGR2-Brd 32

Plasmid

#219014

Purpose

Transcription factor EGR2 with a specific barcode assigned.

Depositor

Filipe Pereira

Publication

Kurochkin et al Cell Syst. 2026 Jan 21;17(1):101457. doi

Insert

EGR2 (EGR2 Human)

Use

Lentiviral

Promoter

The spleen focus-forming virus (SFFV) promoter

Available since

Oct. 29, 2025

Availability

Academic Institutions and Nonprofits only

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SFFV-GATA2-Brd 9

Plasmid

#218993

Purpose

Transcription factor GATA2 with a specific barcode assigned.

Depositor

Filipe Pereira

Publication

Kurochkin et al Cell Syst. 2026 Jan 21;17(1):101457. doi

Insert

GATA2 (GATA2 Human)

Use

Lentiviral

Promoter

The spleen focus-forming virus (SFFV) promoter

Available since

Oct. 29, 2025

Availability

Academic Institutions and Nonprofits only

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SFFV-NFKBIA-Brd 17

Plasmid

#219000

Purpose

Transcription factor NFKBIA with a specific barcode assigned.

Depositor

Filipe Pereira

Publication

Kurochkin et al Cell Syst. 2026 Jan 21;17(1):101457. doi

Insert

NFKBIA (NFKBIA Human)

Use

Lentiviral

Promoter

The spleen focus-forming virus (SFFV) promoter

Available since

Oct. 29, 2025

Availability

Academic Institutions and Nonprofits only

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Bxb1-GA-CpG-free-mStayGold

Plasmid

#241386

Purpose

Bxb1-GA donor plasmid for integrating a transcription unit for constitutive expression of mStayGold with CpG-free promoter

Depositor

Richard Davis

Publication

Blanch-Asensio et al Nat Biomed Eng. 2026 Apr 30. doi: 10.103

Insert

mStayGold

Expression

Mammalian

Promoter

CpG-free

Available since

Sept. 15, 2025

Availability

Academic Institutions and Nonprofits only

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Reckleen_3plus_sgRNA_ptet_pos5a

Plasmid

#233461

Purpose

Level 1 part, Ptet sfgfp dropout sgRNA for position 5a, for building RECKLEEN plasmid with multiple sgRNAs

Depositor

Anke Becker

Publication

Elsayed et al Commun Biol. 2025 Oct 30;8(1):1509. doi:

Insert

Level 1 part, Ptet sfgfp dropout sgRNA for position 5a, ColE1 ori, and KanR.

Use

CRISPR

Available since

April 24, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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Reckleen_3plus_sgRNA_ptet_pos5b

Plasmid

#233464

Purpose

Level 1 part, Ptet sfgfp dropout sgRNA for position 5b, for building RECKLEEN plasmid with multiple sgRNAs

Depositor

Anke Becker

Publication

Elsayed et al Commun Biol. 2025 Oct 30;8(1):1509. doi:

Insert

Level 1 part, Ptet sfgfp dropout sgRNA for position 5b, ColE1 ori, and KanR.

Use

CRISPR

Available since

April 24, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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Bxb1-GA_CAG-TurboGFP

Plasmid

#229781

Purpose

Bxb1-GA donor plasmid for integrating a transcription unit for constitutive expression of nuclear-localised TurboGFP

Depositor

Richard Davis

Publication

Blanch-Asensio et al Nat Biomed Eng. 2026 Apr 30. doi: 10.103

Insert

TurboGFP

Use

Synthetic Biology

Tags

NLS

Expression

Mammalian

Promoter

CAG

Available since

March 6, 2025

Availability

Academic Institutions and Nonprofits only

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