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Plasmid#171912PurposePromoter SPLc17 and RBSc123 in pStA0DepositorInsertPromoter SPLc17 and RBSc123
UseSynthetic BiologyTagsExpressionMutationN/APromoterAvailable sinceFeb. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pStA0::SPLc3-RBSc21
Plasmid#171884PurposePromoter SPLc3 and RBSc21 in pStA0DepositorInsertPromoter SPLc3 and RBSc21
UseSynthetic BiologyTagsExpressionMutationN/APromoterAvailable sinceFeb. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T-1-Staufen-dsRBD(+6)
Plasmid#171546PurposeEncoding part of the Drosophila Staufen protein from M571 to P656DepositorInsertStaufen (stau Fly)
UseTagsExpressionBacterialMutationa six amino acid_TRLTEG_insertion in the L1 regionPromoterAvailable sinceAug. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
ymTq2-ystagRFP pDRF1-GW
Plasmid#168070PurposeFRET fusion between ymTq2 and ystagRFP for use in yeastDepositorInsertymTq2-ystagRFPt
UseTagsExpressionYeastMutationPromoterAvailable sinceJune 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
Cyterm-ystagRFP pDRF1-GW
Plasmid#168061PurposeExpresses Cyterm-ystagRFP to check for monomericity.DepositorInsertCyterm-ystagRFP
UseTagsExpressionYeastMutationPromoterAvailable sinceJune 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-rUSP19ER-siRNAresistant
Plasmid#155251Purposefor mammalian overexpression of the siRNA resistant ER isoform of rat USP19DepositorInsertUSP19 (ER isoform) siRNA resistant (Usp19 Rat)
UseTags6XHis-3XFlagExpressionMammalianMutationLys(23)AAG to AAA, Lys(24)AAG to AAA, Gln(26)CAG …PromoterCMVAvailable sinceOct. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-rUSP19CYT-siRNAresistant
Plasmid#155252Purposefor mammalian overexpression of the siRNA resistant CYT isoform of rat USP19DepositorInsertUSP19 (CYT isoform) siRNA resistant (Usp19 Rat)
UseTags6XHis-3XFlagExpressionMammalianMutationLys(23)AAG to AAA, Lys(24)AAG to AAA, Gln(26)CAG …PromoterCMVAvailable sinceOct. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSTAP-seq_fly-4xUAS
Plasmid#125149PurposeVector to measure the activity of CP candidates in response to a tethered (4xUAS) GAL4-DBD-COF by determining the abundance of transcripts originating from each candidate in fly cellsDepositorInsert4 x upstream activating sequence (UAS)
UseStap-seq screening vectorTagsExpressionMutationPromoterAvailable sinceAug. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pNOC-stacked-GeNL-Ca
Plasmid#101003PurposeConstruct for fusion expression of GeNL calcium reporter and neomycin/G418 resistance gene by a bidirectional promoterDepositorTypeEmpty backboneUseAlgae, nannochloropsis expressionTagsExpressionMutationPromoterAvailable sinceMay 31, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDONR223 SCAF4_CRISPR resistant
Plasmid#122465PurposepDONR for Gateway cloningDepositorInsertSCAF4 CRISPR resistant (SCAF4 Human)
UseGateway cloningTagsExpressionMutationSynonymous mutations conferring resistance to gRN…PromoterAvailable sinceMay 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
hSTARR-seq_SCP1 vector_blocking 2
Plasmid#99317PurposeVector to measure enhancer activity of candidates from a DNA library by STARR-seq.DepositorTypeEmpty backboneUseHstarr-seq screening vectorTagsExpressionMammalianMutationPromoterAvailable sinceSept. 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSTAP-seq_fly-zfh1_spikeIn-1
Plasmid#86387PurposeExpression vector for a transcript origination from a D. pse core promoter, which is used as trasfection control (normalization), i.e. STAP-seq screening vector containing one specific core promoterDepositorInsertD. melanogaster zfh1 enhancer & D. pseudoobscura eve core promoter
UseStap-seq spike in vectorTagsExpressionMutationPromoterAvailable sinceSept. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTALE-STAR-KRAB-C
Plasmid#87540PurposeDestination vector for TALE-KRAB constructionDepositorTypeEmpty backboneUseSynthetic Biology and TALEN; Tale-tf (repressor)TagsExpressionMutationPromoterAvailable sinceJuly 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTALE-STAR-KRAB-G
Plasmid#87541PurposeDestination vector for TALE-KRAB constructionDepositorTypeEmpty backboneUseSynthetic Biology and TALEN; Tale-tf (repressor)TagsExpressionMutationPromoterAvailable sinceJuly 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTALE-STAR-KRAB-T
Plasmid#87538PurposeDestination vector for TALE-KRAB constructionDepositorTypeEmpty backboneUseSynthetic Biology and TALEN; Tale-tf (repressor)TagsExpressionMutationPromoterAvailable sinceJuly 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTALE-STAR-KRAB-A
Plasmid#87539PurposeDestination vector for TALE-KRAB constructionDepositorTypeEmpty backboneUseSynthetic Biology and TALEN; Tale-tf (repressor)TagsExpressionMutationPromoterAvailable sinceJuly 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSTAP-seq_fly-zfh1_spikeIn-3
Plasmid#86389PurposeExpression vector for a transcript origination from a D. pse core promoter, which is used as trasfection control (normalization), i.e. STAP-seq screening vector containing one specific core promoterDepositorInsertD. melanogaster zfh1 enhancer & D. pseudoobscura ush core promoter -TSS#1
UseStap-seq spike in vectorTagsExpressionMutationPromoterAvailable sinceMarch 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSTAP-seq_fly-zfh1_spikeIn-4
Plasmid#86390PurposeExpression vector for a transcript origination from a D. pse core promoter, which is used as trasfection control (normalization), i.e. STAP-seq screening vector containing one specific core promoterDepositorInsertD. melanogaster zfh1 enhancer & D. pseudoobscura ush core promoter -TSS#2
UseStap-seq spike in vectorTagsExpressionMutationPromoterAvailable sinceMarch 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSTAP-seq_fly-ssp3
Plasmid#86382PurposeVector to measure enhancer responsiveness of candidates from a genomic library (cloned instead of the core promoter) by determining the abundance of transcripts originating from each candidate.DepositorInsertD. melanogaster ssp3 enhancer
UseStap-seq screening vectorTagsExpressionMutationPromoterAvailable sinceMarch 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSTAP-seq_fly-ham
Plasmid#86383PurposeVector to measure enhancer responsiveness of candidates from a genomic library (cloned instead of the core promoter) by determining the abundance of transcripts originating from each candidate.DepositorInsertD. melanogaster ham enhancer
UseStap-seq screening vectorTagsExpressionMutationPromoterAvailable sinceMarch 27, 2017AvailabilityAcademic Institutions and Nonprofits only