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Plasmid#62245Purposeused to characterize the promoter activities of PTet for dCas9 and sgRNA response functions.DepositorInsertYFP
UseTagsExpressionBacterialMutationPromoterPtetAvailable sinceApril 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLITMUS-rpoN-cI5G6G-J23106-geneIII
Plasmid#80906PurposePhagemid (encodes cI5G6G and M13 gene III)DepositorInsertsM13 gene III
cI5G6G
UseTagsExpressionBacterialMutationPromoterBBa_J23106 and rpoNAvailable sinceDec. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLITMUS-rpoN-cI5G6T-J23106-geneIII
Plasmid#80907PurposePhagemid (encodes cI5G6T and M13 gene III)DepositorInsertsM13 gene III
cI5G6T
UseTagsExpressionBacterialMutationPromoterBBa_J23106 and rpoNAvailable sinceDec. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLITMUS-rpoN-cI4A5T6T-J23106-geneIII
Plasmid#80908PurposePhagemid (encodes cI4A5T6T and M13 gene III)DepositorInsertsM13 gene III
cI4A5T6T
UseTagsExpressionBacterialMutationPromoterBBa_J23106 and rpoNAvailable sinceDec. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAN-AND
Plasmid#62308Purposesynthetic circuit: AND gateDepositorInsertsA1NT sgRNA
A4NT sgRNA
A2NT sgRNA
A1NT
UseCRISPRTagsExpressionBacterialMutationPromoterPA2, PPhlF, pA4, and pBADAvailable sinceApril 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
LI LacZ
Plasmid#104528PurposeLI lacZ moduleDepositorInsertlacZ
UseCloning vectorTagsExpressionMutationPromoterAvailable sinceOct. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBad_crtEBI_27
Plasmid#63806Purposeexpresses crtEBI genes for neurosporene productionDepositorInsertcrtEBI operon
UseTagsExpressionBacterialMutationCodon optimized for E.coliPromoterPBADAvailable sinceApril 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAN-PA4-RFP
Plasmid#62250Purposereporter plasmid that encodes one of five synthetic sgRNA-repressible promoters that express mRFP1DepositorInsertmRFP
UseTagsExpressionBacterialMutationPromoterpA4Available sinceApril 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAN-PA3-RFP
Plasmid#62249Purposereporter plasmid that encodes one of five synthetic sgRNA-repressible promoters that express mRFP1DepositorInsertmRFP
UseTagsExpressionBacterialMutationPromoterpA3Available sinceApril 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTK713
Plasmid#114699PurposeExpress NuMA(1-2115ΔNLS)-RFP-Nano from Rosa 26 locusDepositorInsertNuMA(1-2115ΔNLS)-RFP-Nano
UseTagsExpressionMutationPromoterAvailable sinceApril 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pWT060e
Plasmid#107905PurposeW2m.0-3, W2m.3-2 plasmid. Contains U6-sgRNA B (CCR5) and is part of the CAMERA2m system for cellular recording in mammalian cellsDepositorInsertU6-sgRNA B (CCR5)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTK729
Plasmid#114710PurposeExpress NuMA(1-705+1700-2115ΔNLS)-RFP-Nano from Rosa 26 locusDepositorInsertNuMA(1-705+1700-2115ΔNLS)-RFP-Nano
UseTagsExpressionMutationPromoterAvailable sinceApril 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBad_crtEBI_2
Plasmid#63803Purposeexpresses crtEBI genes for neurosporene productionDepositorInsertcrtEBI operon
UseTagsExpressionBacterialMutationCodon optimized for E.coliPromoterPBADAvailable sinceApril 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
pWT019b
Plasmid#107894PurposeW1.2 plasmid. Contains PTetO-sd2U-Cas9, PLacO-sgRNA1, PRha-sgRNA2 (sgRNA1-G19T-G20C) and is part of CAMERA 1.2DepositorInsertPTetO-sd2U-Cas9, PLacO-sgRNA1, PRha-sgRNA2 (sgRNA1-G19T-G20C)
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAN-PBAD-YFP
Plasmid#62246Purposeused to characterize the promoter activities of PBAD for dCas9 and sgRNA response functions.DepositorInsertYFP
UseTagsExpressionBacterialMutationPromoterpBADAvailable sinceApril 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBad_crtEBI_52
Plasmid#63808Purposeexpresses crtEBI genes for neurosporene productionDepositorInsertcrtEBI operon
UseTagsExpressionBacterialMutationCodon optimized for E.coliPromoterPBADAvailable sinceApril 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBad_crtEBI_37
Plasmid#63807Purposeexpresses crtEBI genes for neurosporene productionDepositorInsertcrtEBI operon
UseTagsExpressionBacterialMutationCodon optimized for E.coliPromoterPBADAvailable sinceApril 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAN-OR
Plasmid#62307Purposesynthetic circuit: OR gateDepositorInsertsA2NT sgRNA
A4NT sgRNA
A2NT sgRNA
UseCRISPRTagsExpressionBacterialMutationPromoterPA2, PPhlF, and pBADAvailable sinceApril 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
PMT-mApple_2
Plasmid#203772PurposeBrightness calibrationDepositorInsertPMT-mApple dimer
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-3xGFP-tDeg
Plasmid#191371PurposeOverexpression of 3xGFP-tDeg in human cellsDepositorInsert3xGFP-tDeg
UseTags3xGFPExpressionMammalianMutationPromoterAvailable sinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only