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Plasmid#30121DepositorTypeEmpty backboneUseTagsExpressionInsectMutationPromoterAvailable sinceJune 9, 2011AvailabilityAcademic Institutions and Nonprofits only
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pFastBac Dual α1B/β2B Tubulin
Plasmid#170553PurposeEncodes codon-optimized human α1B and β2B tubulin for expression in and purification from insect cells.DepositorUseTagsAP linker, GS linker (GGSGG), His10 tag, L21 enha…ExpressionInsectMutationPromoterAvailable sinceJune 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET51b-His-TEV-SNAP-tag_fast
Plasmid#167271PurposeOverexpression of the fast variant (E30R) of SNAP-tag in E. coliDepositorInsertSNAPf-tag
UseTagsHis x10ExpressionBacterialMutationE30RPromoterT7Available sinceApril 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-FAS-EGFP-WPRE-pA
Plasmid#37091PurposeExpresses EGFP in cells lacking Cre (Cre-Off, flanked by alternative lox FAS sites)DepositorInsertd1eGFP
UseAAV and Cre/Lox; Cre-offTagsExpressionMutationPromoterEF1aAvailable sinceJuly 27, 2012AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-FAS-TdTomato-WPRE-pA
Plasmid#37092PurposeExpresses tdTomato in cells lacking Cre (Cre-Off, flanked by alternative lox FAS sites)DepositorInsertTdTomato
UseAAV and Cre/Lox; Cre-offTagsExpressionMutationPromoterEF1aAvailable sinceJuly 27, 2012AvailabilityAcademic Institutions and Nonprofits only -
pIG-836_NY-ESO-1_TCR_FAS/OX40
Plasmid#207499PurposeThis plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.DepositorInsertFAS/OX40, NY-ESO-1_TCR (FAS Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJan. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFAST-BAC BAF155/SMARCC1-His
Plasmid#177863PurposeTransfer vector to generate recombinant baculovirus expressing BAF155/SMARCC1-HisDepositorInsertSMARCC1 (SMARCC1 Human)
UseTagsHis and His tag at C-terminus with GGGGS linkerExpressionInsectMutationPromoterpolyhedrinAvailable sinceDec. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pIG-832_NY-ESO-1_TCR_FAS/4-1BB
Plasmid#207495PurposeThis plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.DepositorInsertFAS/4-1BB, NY-ESO-1_TCR (FAS Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJan. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pIG-833_NY-ESO-1_TCR_FAS/CD28
Plasmid#207496PurposeThis plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.DepositorInsertFAS/CD28, NY-ESO-1_TCR (FAS Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJan. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFastBac-GFP-dynein-2(D1091–Q4307)
Plasmid#64064PurposeCodon-optimized human dynein-2 motor domain (D1091–Q4307) for baculovirus expression. 6His-ZZ-GFP N-terminal tag, TEV site to cleave 6His-ZZ.DepositorInsertCytoplasmic dynein-2 (DYNC2H1 Human)
UseTagsGFP, His tag, and ZZ tagExpressionInsectMutationDeleted amino acids 1-1090, extra C-terminal vali…PromoterPolyhedrinAvailable sinceApril 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET51b-His-TEV-CLIP-tag_fast
Plasmid#167275PurposeOverexpression of the fast variant (E30R) of CLIP-tag in E. coliDepositorInsertCLIPf-tag
UseTagsHis x10ExpressionBacterialMutationE30RPromoterT7Available sinceApril 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
hSyn-FAS-DO-ReaChR-mCitrine-WPRE
Plasmid#64035PurposeExpresses ReaChR under hSyn promoter. FAS-DO turns off expression in Cre-positive cells. FAS is compatible with other traditional Flex/DIO constructsDepositorInserthSyn-FAS-DO-ReaChR-mCitrine-WPRE
UseAAVTagsReaChR tagged with mCitrineExpressionMammalianMutationPromoterhSynAvailable sinceMay 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFastBac-M13-6His-KIF1C-GFP
Plasmid#130975PurposeExpression of 6His-KIF1C-GFP in insect cells for protein purification.DepositorInsertKIF1C-GFP (KIF1C Human)
UseTags6His and GFPExpressionInsectMutation5 silent mutations that make this construct RNAi …PromoterPolyhedrinAvailable sinceOct. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFAST-BAC BAF57/SMARCE1-His
Plasmid#177861PurposeTransfer vector to generate recombinant baculovirus expressing BAF57/SMARCE1-His proteinDepositorInsertSMARCE1 (SMARCE1 Human)
UseTagsHis tag at C-terminus with GGGGS linkerExpressionInsectMutationPromoterpolyhedrinAvailable sinceDec. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFastBacM13-8His-ZZ-LTLT-Hook3-SNAPf
Plasmid#130976PurposeExpression of 8His-ZZ-LTLT tagged Hook3-SNAPf in insect cells for protein purification.DepositorInsertHook3-SNAPf (HOOK3 Human)
UseTags8His-ZZ-LTLT and SNAPfExpressionInsectMutationPromoterPolyhedrinAvailable sinceOct. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pWZL Neo Myr Flag FASTK
Plasmid#20485DepositorInsertFASTK (FASTK Human)
UseRetroviralTagsFlag and MyrExpressionMammalianMutationPromoterAvailable sinceOct. 27, 2009AvailabilityAcademic Institutions and Nonprofits only -
pFastBac1-OSF-TRIM5alpha(human)(R332P)
Plasmid#79037PurposeBaculoviral entry vector to produce Bac-to-bac baculovirusesDepositorInsertOSF-TRIM5alpha(human R332P) (TRIM5 Human)
UseTagsOneSTrEP-FLAGExpressionInsectMutationChanged Arg 332 to ProPromoterpolyhedrinAvailable sinceMarch 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGL3-Basic-Fascin-Promoter (-402-0)
Plasmid#89823PurposePromoter luciferase assayDepositorInsertFascin1 (FSCN1 Human)
UseLuciferaseTagsExpressionMutationPromoterSV40Available sinceJuly 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFastBac-His-Flag-Tipin-L195A
Plasmid#23120DepositorInsertTipin (TIPIN Human)
UseTagsFlag- and HisExpressionInsectMutationChanged Leucine 195 to AlaninePromoterAvailable sinceMarch 3, 2010AvailabilityAcademic Institutions and Nonprofits only -
pFastBac HTB His-TEV-rDPP9
Plasmid#217326PurposePurification of rat DPP9 from insect cellsDepositorInsertDPP9
UseTagsHis-TEVExpressionInsectMutationPromoterpolyhedrinAvailable sinceAug. 19, 2024AvailabilityAcademic Institutions and Nonprofits only