Addgene

We narrowed to 916 results for: THY-2

Showing: 441 - 460 of 916 results

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pEGFP-LC3-R70A

Plasmid

#155264

Purpose

Mammalian expression of rat LC3 fused to EGFP

Depositor

David Rubinsztein

Article

Puri et al Dev Cell. 2020 Apr 20;53(2):154-168.e6.

Insert

microtubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)

Use

Tags

pEGFP

Expression

Mammalian

Mutation

Arginine 70 to Alanine

Promoter

Available since

May 18, 2021

Availability

Academic Institutions and Nonprofits only

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pLKO.TRC1.shmNsd1.2, puro

Plasmid

#114447

Purpose

Lentiviral vector for expression of shRNA sequence targeting mouse Nsd1

Depositor

Adrian Bracken

Article

Streubel et al Mol Cell. 2018 Apr 19;70(2):371-379.e5.

Insert

shNsd1.2 (Nsd1 Mouse)

Use

Lentiviral and RNAi

Tags

Expression

Mutation

Promoter

Available since

Sept. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pLKO.TRC1.shmNsd1.1, puro

Plasmid

#114446

Purpose

Lentiviral vector for expression of shRNA sequence targeting mouse Nsd1

Depositor

Adrian Bracken

Article

Streubel et al Mol Cell. 2018 Apr 19;70(2):371-379.e5.

Insert

shNsd1.1 (Nsd1 Mouse)

Use

Lentiviral and RNAi

Tags

Expression

Mutation

Promoter

Available since

Sept. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pSt1374-N-NLS-DNMT3L-L-DNMT3Amut-L-dcas9-NLS

Plasmid

#112209

Purpose

Targeted DNA methylation

Depositor

Xingxu Huang

Article

Lu et al Cell Death Dis. 2020 Feb 3;11(2):85. doi

Insert

DNMT3L-L-DNMT3Amut-L-dcas9 (DNMT3L, DNMT3A Human)

Use

CRISPR

Tags

Expression

Mutation

D10A,H840A,N863A,C710S

Promoter

Available since

Oct. 30, 2020

Availability

Academic Institutions and Nonprofits only

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pSt1374m-EF1alpha-N-NLS-DNMT3L-L-DNMT3A-L-dcas9-NLS

Plasmid

#112214

Purpose

Targeted DNA methylation

Depositor

Xingxu Huang

Article

Lu et al Cell Death Dis. 2020 Feb 3;11(2):85. doi

Insert

DNMT3L-L-DNMT3A-L-dcas9 (DNMT3L, DNMT3A Human)

Use

CRISPR

Tags

mCherry

Expression

Mutation

D10A,H840A,N863A

Promoter

Available since

Aug. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pSt1374m-EF1alpha-N-NLS-DNMT3L-L-DNMT3Amut-L-dcas9-NLS

Plasmid

#112215

Purpose

Targeted DNA methylation

Depositor

Xingxu Huang

Article

Lu et al Cell Death Dis. 2020 Feb 3;11(2):85. doi

Insert

DNMT3L-L-DNMT3Amut-L-dcas9 (DNMT3A, DNMT3L Human)

Use

CRISPR

Tags

mCherry

Expression

Mutation

D10A,H840A,N863A,C710S

Promoter

Available since

Aug. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pPICZ A-SLC-His+MFN2[∆TM,∆HB2]-Strep (SB261)

Plasmid

#227610

Purpose

Inducible coexpression of His-tagged SLC25A46 and Strep-tagged MFN2 lacking its transmembrane region and helical bundle 2 (HB2) in Pichia pastoris

Depositor

Luke Chao

Article

Boopathy et al J Biol Chem. 2024 Aug 31:107740. doi: 10

Inserts

SLC25A46 (SLC25A46 Human)
MFN2 (MFN2 Human)

Use

Tags

10xHis and Twin-StrepTag

Expression

Yeast

Mutation

contains aa 2-400, 706-757 [TM and HB2 deleted]

Promoter

AOX1

Available since

Dec. 6, 2024

Availability

Academic Institutions and Nonprofits only

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pDCAF-B

Plasmid

#113647

Purpose

Demethylates methylxanthines.

Depositor

Jeffrey Barrick

Article

Gutierrez et al Appl Environ Microbiol. 2019 Nov 14;85(2

Insert

ndmB

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Nov. 18, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pDCAF-C

Plasmid

#113648

Purpose

Demethylates methylxanthines.

Depositor

Jeffrey Barrick

Article

Gutierrez et al Appl Environ Microbiol. 2019 Nov 14;85(2

Insert

ndmC

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Nov. 18, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pDCAF-AB

Plasmid

#113649

Purpose

Demethylates methylxanthines.

Depositor

Jeffrey Barrick

Article

Gutierrez et al Appl Environ Microbiol. 2019 Nov 14;85(2

Insert

ndmA and ndmB

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Nov. 18, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pDCAF-AC

Plasmid

#113650

Purpose

Demethylates methylxanthines.

Depositor

Jeffrey Barrick

Article

Gutierrez et al Appl Environ Microbiol. 2019 Nov 14;85(2

Insert

ndmA and ndmC

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Nov. 18, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pDCAF-BC

Plasmid

#113651

Purpose

Demethylates methylxanthines.

Depositor

Jeffrey Barrick

Article

Gutierrez et al Appl Environ Microbiol. 2019 Nov 14;85(2

Insert

ndmB and ndmC

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Nov. 18, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pDCAF-A

Plasmid

#113646

Purpose

Demethylates methylxanthines.

Depositor

Jeffrey Barrick

Article

Gutierrez et al Appl Environ Microbiol. 2019 Nov 14;85(2

Insert

ndmA

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Nov. 18, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pDCAF-ABC

Plasmid

#113652

Purpose

Demethylates methylxanthines.

Depositor

Jeffrey Barrick

Article

Gutierrez et al Appl Environ Microbiol. 2019 Nov 14;85(2

Insert

ndmA and ndmB and ndmC

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Nov. 18, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pSLQ5428_pHR_EF1a-mCherry-P2A-Rfx_Cas13d-2xNLS-3xFLAG

Plasmid

#155305

Purpose

Lentiviral vector encoding Rfx Cas13d fused with 2xNLS, 3xFLAG, and 2A-tagged mCherry.

Depositor

Stanley Qi

Article

Abbott et al Cell. 2020 May 14;181(4):865-876.e12. do

Insert

mCherry 2A-tagged to Ruminococcus flavefaciens XPD3002 Cas13d

Use

Lentiviral

Tags

2xNLS-3xFLAG

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

July 21, 2020

Availability

Academic Institutions and Nonprofits only

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pCS2_IRES2_EGFP_KCNQ2 var4

Plasmid

#173154

Purpose

For mammalian expression of KCNQ2 and creating variants

Depositor

Al George

Article

Simkin et al Elife. 2021 Feb 5;10. pii: 64434. doi: 1

Insert

KCNQ2 (KCNQ2 Human)

Use

Tags

IRES2-EGFP

Expression

Mammalian

Mutation

Promoter

Simian CMV IE94

Available since

Sept. 16, 2021

Availability

Academic Institutions and Nonprofits only

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pABE8e-NG-403Q-Nterm-AAV

Plasmid

#194651

Purpose

N-terminal AAV genome plasmid encoding ABE8e + sgRNA to correct the R403Q mutation in mice

Depositor

David Liu

Article

Reichart et al Nat Med. 2023 Feb;29(2):412-421. doi: 10

Insert

ABE8e-NG N-term

Use

AAV

Tags

BPNLS and NpuN spilt intein

Expression

Mutation

Promoter

TNNT2

Available since

Feb. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pABE8e-NG-403Q-Cterm-AAV

Plasmid

#194652

Purpose

C-terminal AAV genome plasmid encoding ABE8e + sgRNA to correct the R403Q mutation in mice

Depositor

David Liu

Article

Reichart et al Nat Med. 2023 Feb;29(2):412-421. doi: 10

Insert

ABE8e-NG C-term

Use

AAV

Tags

BPNLS and NpuC split intein

Expression

Mutation

Promoter

TNNT2

Available since

Feb. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pCS2_IRES2_CyOFP1_KCNQ2 var4

Plasmid

#173155

Purpose

For mammalian expression of KCNQ2 and creating variants

Depositor

Al George

Article

Simkin et al Elife. 2021 Feb 5;10. pii: 64434. doi: 1

Insert

KCNQ2 (KCNQ2 Human)

Use

Tags

IRES2-CyOFP1

Expression

Mammalian

Mutation

Promoter

Simian CMV IE94

Available since

Sept. 16, 2021

Availability

Academic Institutions and Nonprofits only

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pSLQ5465_pHR_hU6-crScaffold_EF1a-PuroR-T2A-BFP

Plasmid

#155307

Purpose

Rfx Cas13d guide cloning lentiviral vector with constitutively expressed puromycin resistance 2A-tagged with tagBFP

Depositor

Stanley Qi

Article

Abbott et al Cell. 2020 May 14;181(4):865-876.e12. do

Insert

Rfx Cas13d CRISPR RNA puromycin resistance 2A-tagged BFP

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6, EF1a

Available since

July 21, 2020

Availability

Academic Institutions and Nonprofits only

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