Addgene

We narrowed to 6,543 results for: tev

Showing: 4541 - 4560 of 6543 results

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pMH-HT_ygiF-TTM

Plasmid

#127794

Purpose

Expresses the TTM/CYTH domain of ygiF in E. coli cells

Depositor

Michael Hothorn

Article

Lorenzo-Orts et al Life Sci Alliance. 2019 May 27;2(3). pii

Insert

ygiF (ygiF Escherichia coli)

Use

Tags

6xHis tag + TEV cleavage site

Expression

Bacterial

Mutation

N-terminal TTM domain of ygiF (residues 1-200)

Promoter

Available since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pNH-TrxT-G-S10

Plasmid

#127829

Purpose

Production of recombinant human ribosomal protein S10

Depositor

Ivanhoe Leung

Article

Correddu et al Sci Rep. 2019 Jun 20;9(1):8884. doi: 10.

Insert

RPS10 (RPS10 Human)

Use

Tags

His6 - Thioredoxin - TEV

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pCF736

Plasmid

#121893

Purpose

EF1a-dTEV-protease-GFP

Depositor

David Savage

Article

Oakes et al Cell. 2019 Jan 10;176(1-2):254-267.e16.

Inserts

TEV protease
eGFP

Use

Lentiviral

Tags

Expression

Mutation

Promoter

EF-1a core promoter

Available since

March 5, 2019

Availability

Academic Institutions and Nonprofits only

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pTriEX Tom20 mVenus BoPD

Plasmid

#114466

Purpose

Mammalian expression of mitochondrially targeted, mVenus labelled BoPD

Depositor

Andrew Woolley

Article

Reis et al ACS Synth Biol. 2018 Oct 19;7(10):2355-2

Insert

BoPD

Use

Tags

HIS, TEV and FLAG and TOM20 and mVenus

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 25, 2018

Availability

Academic Institutions and Nonprofits only

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pEHIShqprtR161Q

Plasmid

#92413

Purpose

hQPRTase harboring R161Q mutation

Depositor

Huanting Liu, James Naismith

Article

Liu et al J Mol Biol. 2007 Oct 26;373(3):755-63. E

Insert

hQPRTase (QPRT Human)

Use

Tags

TEV protease cleavable 6xHis

Expression

Bacterial

Mutation

R161Q

Promoter

T7

Available since

Nov. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pEHIAhqprtR161G

Plasmid

#92412

Purpose

hQPRTase harbouring R161G mutation

Depositor

Huanting Liu, James Naismith

Article

Liu et al J Mol Biol. 2007 Oct 26;373(3):755-63. E

Insert

hQPRTase (QPRT Human)

Use

Tags

TEV protease cleavable 6xHis

Expression

Bacterial

Mutation

R161G

Promoter

T7

Available since

Aug. 23, 2017

Availability

Academic Institutions and Nonprofits only

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pEHIShqprtR102Q

Plasmid

#92407

Purpose

hQPRTase harboring R102Q mutation

Depositor

Huanting Liu, James Naismith

Article

Liu et al J Mol Biol. 2007 Oct 26;373(3):755-63. E

Insert

hQPRTase (QPRT Human)

Use

Tags

TEV protease cleavable 6xHis

Expression

Bacterial

Mutation

R102Q

Promoter

T7

Available since

Aug. 17, 2017

Availability

Academic Institutions and Nonprofits only

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pEHIShqprtK171S

Plasmid

#92404

Purpose

hQPRTase harbouring K171S mutation

Depositor

Huanting Liu, James Naismith

Article

Liu et al J Mol Biol. 2007 Oct 26;373(3):755-63. E

Insert

hQPRTase (QPRT Human)

Use

Tags

TEV protease cleavable 6xHis

Expression

Bacterial

Mutation

K171S

Promoter

T7

Available since

Aug. 8, 2017

Availability

Academic Institutions and Nonprofits only

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pEHIShqprtR138Q

Plasmid

#92411

Purpose

hQPRTase harbouring R138Q mutation

Depositor

Huanting Liu, James Naismith

Article

Liu et al J Mol Biol. 2007 Oct 26;373(3):755-63. E

Insert

hQPRTase (QPRT Human)

Use

Tags

TEV protease cleavable 6xHis

Expression

Bacterial

Mutation

R138Q

Promoter

T7

Available since

Aug. 8, 2017

Availability

Academic Institutions and Nonprofits only

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pEHIShqprtK139A

Plasmid

#92403

Purpose

hQPRTase harbouring K139A mutation

Depositor

Huanting Liu, James Naismith

Article

Liu et al J Mol Biol. 2007 Oct 26;373(3):755-63. E

Insert

hQPRTase (QPRT Human)

Use

Tags

TEV cleacable 6xHis

Expression

Bacterial

Mutation

K139A

Promoter

T7

Available since

Aug. 8, 2017

Availability

Academic Institutions and Nonprofits only

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pEHIShqprtR102A

Plasmid

#92405

Purpose

hQPRTASE harbouring R102A mutation

Depositor

Huanting Liu, James Naismith

Article

Liu et al J Mol Biol. 2007 Oct 26;373(3):755-63. E

Insert

hQPRTase (QPRT Human)

Use

Tags

TEV protease cleavable 6xHis

Expression

Bacterial

Mutation

R102A

Promoter

T7

Available since

July 26, 2017

Availability

Academic Institutions and Nonprofits only

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pQStrep2-UBL3

Plasmid

#31330

Depositor

Konrad Buessow

Article

Bussow et al Microb Cell Fact. 2005 Jul 5. 4():21.

Insert

ubiquitin-like 3 (UBL3 Human)

Use

Tags

His, StrepII-tag, and TEV

Expression

Bacterial

Mutation

Promoter

Available since

March 22, 2013

Availability

Academic Institutions and Nonprofits only

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pEG634

Plasmid

#40121

Depositor

Geraldine Seydoux

Article

Griffin et al Cell. 2011 Sep 16;146(6):955-68.

Insert

Mex-5 (aa244-468) (mex-5 Nematode)

Use

Gateway destination vector

Tags

Dendra2 PAFP (sequence optimized for worm express…

Expression

Worm

Mutation

contains aa244-468

Promoter

4.4 kb mex-5 promoter fragment

Available since

Sept. 19, 2012

Availability

Academic Institutions and Nonprofits only
pLIC.B3.MJ0130m

Plasmid

#11528

Depositor

Sung-Hou Kim

Article

Kim et al Proc Natl Acad Sci U S A. 2005 Mar 1. 10

Insert

Type I restriction-modification enzyme (MJ_0130m M. jannaschii)

Use

Tags

HisTag, TEV cleavable

Expression

Bacterial

Mutation

Promoter

Available since

April 10, 2006

Availability

Academic Institutions and Nonprofits only
pFastBac(His10-Lckg2a,Y394F)

Plasmid

#177893

Purpose

Baculo expression of His10 tag fused to human LckG2A

Depositor

Ron Vale

Article

Hui et al Nat Struct Mol Biol. 2014 Feb;21(2):133-

Insert

Lck (LCK Human)

Use

Tags

10xHis-TEV

Expression

Insect

Mutation

G2A,Y394F

Promoter

Polyhedrin

Available since

Availability

Academic Institutions and Nonprofits only

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pAAV-S5E2-ChR2-mCherry (AAV9)

Viral Prep

#135634-AAV9

Purpose

Ready-to-use AAV9 particles produced from pAAV-S5E2-ChR2-mCherry (#135634). In addition to the viral particles, you will also receive purified pAAV-S5E2-ChR2-mCherry plasmid DNA. Expression of ChR2-mCherry under the control of the E2 regulatory element. These AAV preparations are suitable purity for injection into animals.

Depositor

Jordane Dimidschstein

Article

Vormstein-Schneider et al Nat Neurosci. 2020 Dec;23(12):1629-1636.

Promoter

Tags

mCherry

Available since

Sept. 15, 2021

Availability

Academic Institutions and Nonprofits only
pAAV-S5E2-Gq-P2A-dTomato (AAV1)

Viral Prep

#135635-AAV1

Purpose

Ready-to-use AAV1 particles produced from pAAV-S5E2-Gq-P2A-dTomato (#135635). In addition to the viral particles, you will also receive purified pAAV-S5E2-Gq-P2A-dTomato plasmid DNA. Expression of Gq-DREADD and dTomato (physically separate) under the control of the E2 regulatory element. These AAV preparations are suitable purity for injection into animals.

Depositor

Jordane Dimidschstein

Article

Vormstein-Schneider et al Nat Neurosci. 2020 Dec;23(12):1629-1636.

Promoter

Tags

dTomato (physically separate, not a fusion protein)

Available since

Nov. 29, 2021

Availability

Academic Institutions and Nonprofits only
pAAV-S5E2-dTom-nlsdTom (AAV9)

Viral Prep

#135630-AAV9

Purpose

Ready-to-use AAV9 particles produced from pAAV-S5E2-dTom-nlsdTom (#135630). In addition to the viral particles, you will also receive purified pAAV-S5E2-dTom-nlsdTom plasmid DNA. Bicistronic expression of dTomato and nuclear-targeted dTomato under the control of the E2 regulatory element. These AAV preparations are suitable purity for injection into animals.

Depositor

Jordane Dimidschstein

Article

Vormstein-Schneider et al Nat Neurosci. 2020 Dec;23(12):1629-1636.

Promoter

Tags

dTomato

Available since

Nov. 29, 2021

Availability

Academic Institutions and Nonprofits only
pAAV-S5E2-GFP-fGFP (AAV1)

Viral Prep

#135631-AAV1

Purpose

Ready-to-use AAV1 particles produced from pAAV-S5E2-GFP-fGFP (#135631). In addition to the viral particles, you will also receive purified pAAV-S5E2-GFP-fGFP plasmid DNA. Bicistronic expression of GFP and membrane-targeted GFP under the control of the E2 regulatory element. These AAV preparations are suitable purity for injection into animals.

Depositor

Jordane Dimidschstein

Article

Vormstein-Schneider et al Nat Neurosci. 2020 Dec;23(12):1629-1636.

Promoter

Tags

EGFP

Available since

Sept. 15, 2021

Availability

Academic Institutions and Nonprofits only
pAAV-S5E2-dTom-nlsdTom (AAV1)

Viral Prep

#135630-AAV1

Purpose

Ready-to-use AAV1 particles produced from pAAV-S5E2-dTom-nlsdTom (#135630). In addition to the viral particles, you will also receive purified pAAV-S5E2-dTom-nlsdTom plasmid DNA. Bicistronic expression of dTomato and nuclear-targeted dTomato under the control of the E2 regulatory element. These AAV preparations are suitable purity for injection into animals.

Depositor

Jordane Dimidschstein

Article

Vormstein-Schneider et al Nat Neurosci. 2020 Dec;23(12):1629-1636.

Promoter

Tags

dTomato

Available since

Jan. 6, 2022

Availability

Academic Institutions and Nonprofits only