Addgene

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Showing: 4541 - 4560 of 12099 results

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pET14b-PITPalpha K61A

Plasmid

#58258

Purpose

bacterial expression of human PITP alpha K61A

Depositor

Shamshad Cockcroft

Article

Tilley et al Structure. 2004 Feb;12(2):317-26.

Insert

PITP alpha (PITPNA Human)

Use

Tags

HIS

Expression

Bacterial

Mutation

K61A (abolishes PI binding)

Promoter

T7

Available since

July 15, 2014

Availability

Academic Institutions and Nonprofits only
Danio rerio cryaa V62T

Plasmid

#47630

Purpose

Recombinant protein production

Depositor

Mason Posner

Article

Posner et al PLoS One. 2012;7(3):e34438. doi: 10.1371

Insert

Alpha A-crystallin (cryaa Zebrafish)

Use

Tags

Expression

Bacterial

Mutation

Changed Valine 62 to Threonine

Promoter

T7

Available since

Oct. 21, 2013

Availability

Academic Institutions and Nonprofits only

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Danio rerio cryaa T147V

Plasmid

#47628

Purpose

Recombinant protein production

Depositor

Mason Posner

Article

Posner et al PLoS One. 2012;7(3):e34438. doi: 10.1371

Insert

Alpha A-crystallin (cryaa Zebrafish)

Use

Tags

Expression

Bacterial

Mutation

Changed Theronine 147 to Valine

Promoter

T7

Available since

Sept. 30, 2013

Availability

Academic Institutions and Nonprofits only

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Danio rerio cryaa C143S

Plasmid

#47619

Purpose

Recombinant protein production

Depositor

Mason Posner

Article

Posner et al PLoS One. 2012;7(3):e34438. doi: 10.1371

Insert

Alpha A-crystallin (cryaa Zebrafish)

Use

Tags

Expression

Bacterial

Mutation

Cysteine 143 to Serine

Promoter

T7

Available since

Sept. 30, 2013

Availability

Academic Institutions and Nonprofits only

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pLEX_970_puro_DEST_V5_TLOC1 (220aa)

Plasmid

#45287

Depositor

William Hahn

Article

Hagerstrand et al Cancer Discov. 2013 Jun 13. [Epub ahead

Insert

TLOC1 (SEC62 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

Promoter

Available since

May 22, 2013

Availability

Academic Institutions and Nonprofits only
pUASp YFP Rab14 DN

Plasmid

#37715

Depositor

Matthew Scott

Article

Zhang et al Genetics. 2007 Jun;176(2):1307-22. Epub

Insert

Rab14 (Rab14 Fly)

Use

Drosophila

Tags

YFP

Expression

Insect

Mutation

S49N (dominant negative)

Promoter

GAL4

Available since

March 13, 2013

Availability

Academic Institutions and Nonprofits only
MET FLAG72QDProCFPp303

Plasmid

#15590

Depositor

Susan Lindquist

Article

Duennwald et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

Insert

htt 72Q Delta Pro (HTT Human)

Use

Tags

CFP and FLAG

Expression

Yeast

Mutation

Proline-rich region removed

Promoter

Available since

Aug. 15, 2007

Availability

Academic Institutions and Nonprofits only
pmCell-Free_KA2_ZIKV NS2B

Plasmid

#145364

Purpose

Cell-free/mammalian expression vector for Zika virus NS2B protein with N-term mCherry

Depositor

Rob Parton

Article

Varasteh Moradi et al J Proteome Res. 2020 Apr 3;19(4):1522-15

Insert

ZIKV NS2B

Use

Tags

8xHis and mCherry

Expression

Bacterial

Mutation

Promoter

T7- CMV/CAG

Available since

Availability

Academic Institutions and Nonprofits only

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p3xFLAG-EYFP

Plasmid

#34582

Depositor

Celeste Nelson

Article

Lee et al EMBO J. 2011 May 24;30(13):2662-74. doi:

Type

Empty backbone

Use

Tags

3xFLAG and EYFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 3, 2012

Availability

Academic Institutions and Nonprofits only

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pM5MF529

Plasmid

#17686

Depositor

David Shalloway

Article

Lin et al Oncogene. 1995 Jan 19. 10(2):401-5.

Insert

c-src Y529F (Src Mouse)

Use

Tags

Expression

Mammalian

Mutation

Tyr 529 changed to Phe .

Promoter

Available since

April 24, 2008

Availability

Academic Institutions and Nonprofits only

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pWZ414-F22

Plasmid

#23080

Depositor

Sharon Dent

Article

Matangkasombut et al Mol Cell. 2003 Feb . 11(2):353-63.

Insert

yeast Histone H3-2 and Histone H4-2

Use

Tags

Expression

Yeast

Mutation

Histone H4 changed Lysine 5 to Arginine, H4 K5R.

Promoter

Available since

Feb. 17, 2010

Availability

Academic Institutions and Nonprofits only
pWZ414-F24

Plasmid

#23081

Depositor

Sharon Dent

Article

Matangkasombut et al Mol Cell. 2003 Feb . 11(2):353-63.

Insert

yeast Histone H3-2 and Histone H4-2

Use

Tags

Expression

Yeast

Mutation

Histone H4 changed Lysine 16 to Arginine, H4 K16R.

Promoter

Available since

Feb. 17, 2010

Availability

Academic Institutions and Nonprofits only
pWZ414-F25

Plasmid

#23082

Depositor

Sharon Dent

Article

Matangkasombut et al Mol Cell. 2003 Feb . 11(2):353-63.

Insert

yeast Histone H3-2 and Histone H4-2

Use

Tags

Expression

Yeast

Mutation

Histone H4 changed lysine 16 to Glutamine, H4 K16…

Promoter

Available since

Feb. 17, 2010

Availability

Academic Institutions and Nonprofits only
pWZ414-F26

Plasmid

#23083

Depositor

Sharon Dent

Article

Matangkasombut et al Mol Cell. 2003 Feb . 11(2):353-63.

Insert

yeast Histone H3-2 and Histone H4-2

Use

Tags

Expression

Yeast

Mutation

Histone 4 changed Lysine 16 to glycine, H4 K16G.

Promoter

Available since

Feb. 17, 2010

Availability

Academic Institutions and Nonprofits only
pFuse-STOP

Plasmid

#217677

Purpose

Empty vector control plasmid containing a stop codon prior to the Fc encoding region

Depositor

Stephen Blacklow

Article

Dates et al bioRxiv 2022.07.14.500097

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 16, 2024

Availability

Academic Institutions and Nonprofits only

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pAN1717

Plasmid

#74696

Purpose

RPU standard plasmid

Depositor

Christopher Voigt

Article

Nielsen et al Science. 2016 Apr 1;352(6281):aac7341. d

Insert

Insulated J23101-YFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 2, 2016

Availability

Academic Institutions and Nonprofits only

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pAsylum

Plasmid

#24754

Depositor

Christopher Buck

Article

Schowalter et al Cell Host Microbe. 2010 Jun 25;7(6):509-

Type

Empty backbone

Use

Cloning vector

Tags

Expression

Mutation

Promoter

Available since

June 21, 2010

Availability

Academic Institutions and Nonprofits only

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pAM238

Plasmid

#226639

Purpose

S. cerevisiae ubiquitin

Depositor

Andreas Martin

Article

Lander et al Nature. 2012 Jan 11;482(7384):186-91. do

Insert

Ubiquitin (UBI4 Budding Yeast)

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Oct. 29, 2024

Availability

Academic Institutions and Nonprofits only

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RT-1

Plasmid

#220434

Purpose

Base construct used to create PUP2/3HA fusion proteins in S. cerevisiae. (pBSII/PUP2/3HA/URA3)

Depositor

Marvin Wickens

Article

Lapointe et al Nat Methods. 2015 Dec;12(12):1163-70. do

Type

Empty backbone

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

June 21, 2024

Availability

Academic Institutions and Nonprofits only

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SNAP-Sec61B

Plasmid

#141152

Purpose

Fluorescent labeling of ER membrane in mammalian cells

Depositor

Gia Voeltz

Article

Abrisch et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

Insert

Sec61 beta (SEC61B Human)

Use

Tags

SNAP tag

Expression

Mammalian

Mutation

L79M on Sec61B

Promoter

CMV

Available since

Aug. 31, 2020

Availability

Academic Institutions and Nonprofits only

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