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pTRANS_232

Plasmid

#91121

Purpose

Transformation, Type: T-DNA, Plant Selection: 2x35S:bar, Viral Replication: ToLCV

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

35S

Available since

Sept. 29, 2017

Availability

Academic Institutions and Nonprofits only

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pCMP-APr

Plasmid

#61184

Purpose

Fluorescent reporter for apoplast MtBCP1sp-mCherry expressed under MtPT4 promoter

Depositor

Maria Harrison

Article

Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

Insert

MtBCPsp-mCherry

Use

Tags

mCherry

Expression

Plant

Mutation

Promoter

MtPT4

Available since

April 23, 2015

Availability

Academic Institutions and Nonprofits only

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S13_EcoRV_UBC_AscI

Plasmid

#128477

Purpose

UBC promoter

Depositor

Lindy Barrett

Article

Hazelbaker et al Sci Rep. 2020 Jan 20;10(1):635. doi: 10.

Insert

UBC promoter

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pH7m34GW_RcCHAD-mCherry

Plasmid

#127784

Purpose

RcCHAD-mCherry expression in plants

Depositor

Michael Hothorn

Article

Lorenzo-Orts et al Life Sci Alliance. 2019 May 27;2(3). pii

Insert

Ubi10p:RcCHAD-mCherry

Use

Tags

mCherry

Expression

Plant

Mutation

Promoter

Ubiquitin10 promoter + 5' UTR

Available since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pKLV2-EF1aMS2p65HSF1hyg-W

Plasmid

#112920

Purpose

Lentiviral vector expressing MS2p65HSF1 under the control of the long human EF1a promoter

Depositor

Kosuke Yusa

Article

Chong et al Genome Biol. 2018 Nov 26;19(1):205. doi:

Insert

MS2p65HSF1-T2Ahyg

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Human EF1a promoter

Available since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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TfRΔEctoR1-eGFP A206K

Plasmid

#113554

Purpose

Transmembrane model receptor to probe receptor internalization via clathrin mediated pathway

Depositor

Jeanne Stachowiak

Article

DeGroot et al Biophys J. 2018 Mar 27;114(6):1377-1388.

Insert

TfRΔEctoR1-eGFP A206K

Use

Tags

Expression

Mammalian

Mutation

eGFP A206K

Promoter

Available since

July 25, 2018

Availability

Academic Institutions and Nonprofits only

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VAMP8-SuperNova

Plasmid

#131855

Purpose

Expresses SuperNova fused to endosomal SNARE VAMP8

Depositor

Geert van den Bogaart

Article

Paardekooper et al Int J Mol Sci. 2019 Aug 25;20(17). pii:

Insert

SuperNova

Use

Tags

Myc-tag and VAMP8 (mouse sequence)

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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MTK2_013

Plasmid

#123705

Purpose

Encodes a spacer (for multicistronic) as a Type 2 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

pSpacer multicistronic

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pc3XB-ZF102

Plasmid

#13236

Depositor

Daniel Voytas

Article

Wright et al Nat Protoc. 2006;1(3):1637-52. doi: 10.1

Insert

ZF102

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 31, 2006

Availability

Academic Institutions and Nonprofits only

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EZ-L767

Plasmid

#131773

Purpose

PADH1_sFUS_Fusion Red_PixD_TACT1

Depositor

Jared Toettcher

Article

Zhao et al Nat Chem Biol. 2019 Jun;15(6):589-597. d

Insert

PADH1_sFUS_Fusion Red_PixD_TACT1

Use

Integration into δ-sites

Tags

Expression

Yeast

Mutation

Promoter

PADH1

Available since

Sept. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pTNT-pT7-tetR-sfGFP

Plasmid

#140868

Purpose

T7 promoter driven synthesis of C-terminal fusion of sfGFP to TetR repressor (TetR-sfGFP)

Depositor

Neal Devaraj

Article

Niederholtmeyer et al Nat Commun. 2018 Nov 28;9(1):5027. doi:

Insert

TetR-sfGFP expressed with T7 promoter

Use

Synthetic Biology

Tags

sfGFP

Expression

Bacterial

Mutation

Promoter

T7

Available since

May 7, 2020

Availability

Academic Institutions and Nonprofits only

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MTK3b_004

Plasmid

#123785

Purpose

Encodes mAzamiGreen as a Type 3b part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

mAzamiGreen

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pISH-Neurod1

Plasmid

#74396

Purpose

Use to synthesize riboprobes for in situ hybridization (ISH) probe for Neurod1. Not for gene expression.

Depositor

Peter Mombaerts

Article

Chang et al Gene Expr Patterns. 2016 Jul;21(2):69-80

Insert

neurogenic differentiation 1 (Neurod1 Mouse)

Use

Tags

Expression

Mutation

Promoter

Available since

April 13, 2016

Availability

Academic Institutions and Nonprofits only

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pCFJ1656

Plasmid

#51483

Purpose

miniMos transposable element with HygroR and peel-1, R4-R3 Gateway cloning

Depositor

Erik Jorgensen

Article

Frokjaer-Jensen et al Nat Methods. 2014 May;11(5):529-34. doi:

Type

Empty backbone

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

Feb. 28, 2014

Availability

Academic Institutions and Nonprofits only

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pLR5-CAG-NLS-tdiRFP670

Plasmid

#82621

Purpose

Expresses NLS-tdiRFP670 in mammalian cells

Depositor

Hiroshi Ochiai

Article

Seirin-Lee et al PLoS Comput Biol. 2019 Sep 11;15(9):e100

Insert

NLS-tdiRFP670

Use

Piggybac vector

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 12, 2019

Availability

Academic Institutions and Nonprofits only

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pHH0103_ARFGEF2_Sec7

Plasmid

#109924

Purpose

Protein expression and purification of ARFGEF2_Sec7

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

ARFGEF2_Sec7

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pECFP-Sec23A

Plasmid

#66610

Purpose

CFP fusion of Sec23A

Depositor

David Stephens

Article

Stephens et al J Cell Sci. 2000 Jun;113 ( Pt 12):2177-8

Insert

Sec23A (SEC23A Human)

Use

Tags

ECFP

Expression

Mammalian

Mutation

Promoter

Available since

July 28, 2015

Availability

Academic Institutions and Nonprofits only
pSLIK 3XFLAG-EGFP-NLS hygro

Plasmid

#87781

Purpose

Inducible expression of EGFP with a C-terminal NLS sequence

Depositor

Kevin Janes

Article

Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

Insert

eGFP

Use

Lentiviral

Tags

3xFLAG and NLS

Expression

Mammalian

Mutation

Promoter

Available since

May 10, 2017

Availability

Academic Institutions and Nonprofits only

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CAG::PSAMQ79G,Q139G:5HT3HC-IRES-GFP

Plasmid

#32474

Depositor

Scott Sternson

Article

Magnus et al Science. 2011 Sep 2;333(6047):1292-6.

Insert

PSAMQ79G,Q139G:5HT3 HC

Use

Tags

IRES-EGFP

Expression

Mammalian

Mutation

Promoter

CAG

Available since

April 10, 2012

Availability

Academic Institutions and Nonprofits only

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pISH-Neurog1

Plasmid

#74395

Purpose

Use to synthesize riboprobes for in situ hybridization (ISH) probe for Neurog1. Not for gene expression.

Depositor

Peter Mombaerts

Article

Chang et al Gene Expr Patterns. 2016 Jul;21(2):69-80

Insert

neurogenin 1 (Neurog1 Mouse)

Use

Tags

Expression

Mutation

Promoter

Available since

April 13, 2016

Availability

Academic Institutions and Nonprofits only

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