Addgene

We narrowed to 13,023 results for: CAG

Showing: 4541 - 4560 of 13023 results

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Bxb1-GA_AIO-GZV-SynZiFTR_Down-Tandem donor

Plasmid

#229787

Purpose

Bxb1-GA donor plasmid with downstream tandem syntax for all-in-one grazoprevir-inducible expression (empty)

Depositor

Richard Davis

Article

Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

Insert

LacZ; ZF10-NS3-p65

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Feb. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pPBbsr-JNK KTR-mCherry

Plasmid

#115493

Purpose

Expression vector for the JNK activity reporter JNK KTR-mCherry in mammalian cells

Depositor

Kazuhiro Aoki

Article

Miura et al Cell Rep. 2018 Sep 4;24(10):2658-2668. d

Insert

JNK KTR-mCherry

Use

Tags

mCherry

Expression

Mammalian

Mutation

mCherry G229L, mCherry Y298 deleted

Promoter

CAG

Available since

Sept. 26, 2018

Availability

Academic Institutions and Nonprofits only

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pPBbsr2-Lifeact-miRFP703

Plasmid

#178530

Purpose

Expresses Lifeact-miRFP703 in mammalian cells

Depositor

Kazuhiro Aoki

Article

Yamamoto et al Nat Commun. 2021 Dec 8;12(1):7145. doi:

Insert

Lifeact-miRFP703

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Dec. 21, 2021

Availability

Academic Institutions and Nonprofits only

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#1.4 pre-R-SynapShot-TagBFP

Plasmid

#214719

Purpose

Expresses red #1.4 pre-SynapShot and TagBFP

Depositor

Won Do Heo

Article

Son et al Nat Methods. 2024 Jan 8. doi: 10.1038/s4

Insert

#1.4 pre-R-SynapShot

Use

AAV

Tags

TagBFP

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Nov. 22, 2024

Availability

Academic Institutions and Nonprofits only

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#1.4 post-R-SynapShot-Lyn-miRFP670

Plasmid

#214718

Purpose

Expresses red #1.4 post-SynapShot and Lyn-miRFP670

Depositor

Won Do Heo

Article

Son et al Nat Methods. 2024 Jan 8. doi: 10.1038/s4

Insert

#1.4 post-R-SynapShot

Use

Tags

Lyn-miRFP670

Expression

Mammalian

Mutation

Promoter

CAG

Available since

March 21, 2024

Availability

Academic Institutions and Nonprofits only

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AAV-GfaABC1D-Cre-4x6T

Plasmid

#196410

Purpose

Astrocytic expression of Cre recombinase in AAV; astrocyte specificity enhanced with 4x6T miRNA targeting cassette

Depositor

Stanley Thomas Carmichael

Article

Gleichman et al Nat Commun. 2023 Nov 16;14(1):7426. doi:

Has Service

AAV5

Insert

Cre

Use

AAV; Astrocyte-selective

Tags

Expression

Mammalian

Mutation

Promoter

CAG and GfaABC1D

Available since

Feb. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pWN_U6-B2M-miniGag

Plasmid

#228957

Purpose

miniEDV production plasmid. Expresses the miniGag (CAG promoter) and sgRNA (human U6 promoter)

Depositor

Jennifer Doudna

Article

Ngo et al Proc Natl Acad Sci U S A. 2025 Jan 7;122

Insert

miniGag

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 2, 2025

Availability

Academic Institutions and Nonprofits only

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AAV-FLEX-EGFP-mir30(Scn9a)

Plasmid

#79672

Purpose

Cre-dependent knockdown of Scn9a (Nav1.7)

Depositor

Scott Sternson

Article

Branco et al Cell. 2016 Jun 16;165(7):1749-61. doi: 1

Insert

EGFP (Scn9a Mouse, jellyfish)

Use

AAV and Cre/Lox

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

July 27, 2016

Availability

Academic Institutions and Nonprofits only

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pCX-SpiCee-NLS

Plasmid

#140900

Purpose

mammalian expression of SpiCee-NLS, a nucleus-restricted variant of the calcium buffer SpiCee that prevents downstream signaling

Depositor

Xavier Nicol

Article

Ros et al Cell Rep. 2020 Jul 21;32(3):107934. doi:

Insert

SpiCee-NLS

Use

Tags

mRFP

Expression

Mammalian

Mutation

Promoter

CAG

Available since

July 24, 2020

Availability

Academic Institutions and Nonprofits only

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pCX-SpiCee-NES

Plasmid

#140901

Purpose

mammalian expression of SpiCee-NES, a nucleus-excluded variant of the calcium buffer SpiCee that prevents downstream signaling

Depositor

Xavier Nicol

Article

Ros et al Cell Rep. 2020 Jul 21;32(3):107934. doi:

Insert

SpiCee-NES

Use

Tags

mRFP

Expression

Mammalian

Mutation

Promoter

CAG

Available since

July 24, 2020

Availability

Academic Institutions and Nonprofits only

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#2.4 post-G-SynapShot-mScarlet

Plasmid

#214720

Purpose

Expresses green #2.4 post-SynapShot and mScarlet

Depositor

Won Do Heo

Article

Son et al Nat Methods. 2024 Jan 8. doi: 10.1038/s4

Insert

#2.4 post-G-SynapShot

Use

AAV

Tags

mScarlet

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Jan. 23, 2025

Availability

Academic Institutions and Nonprofits only

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pCX-Lyn-SpiCee

Plasmid

#140837

Purpose

mammalian expression of Lyn-SpiCee, a lipid raft-targeted variant of the calcium buffer SpiCee that prevents downstream signaling

Depositor

Xavier Nicol

Article

Ros et al Cell Rep. 2020 Jul 21;32(3):107934. doi:

Insert

Lyn-SpiCee

Use

Tags

mRFP

Expression

Mammalian

Mutation

Promoter

CAG

Available since

July 24, 2020

Availability

Academic Institutions and Nonprofits only

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pWN_U6-TRAC-miniGag

Plasmid

#228960

Purpose

miniEDV production plasmid. Expresses the miniGag polypeptide (CAG promoter) and sgRNA (human U6 promoter)

Depositor

Jennifer Doudna

Article

Ngo et al Proc Natl Acad Sci U S A. 2025 Jan 7;122

Insert

miniGag

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pCas9D10A_GFP

Plasmid

#44720

Purpose

Co-expression of human codon-optimized Cas9 (D10A) mutant nickase and GFP, plasmid optimized for expression in human pluripotent stem cells

Depositor

Kiran Musunuru

Article

Ding et al Cell Stem Cell. 2013 Apr 4;12(4):393-4.

Insert

Cas9D10A-2A-GFP

Use

CRISPR

Tags

2A-GFP

Expression

Mammalian

Mutation

D10A mutation- nickase mutant

Promoter

CAG

Available since

May 8, 2013

Availability

Academic Institutions and Nonprofits only

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pT076

Plasmid

#137880

Purpose

Donor vector for integration at the human AAVS1 safe harbor locus of Doxicycline-inducible dCas9-VPR-T2A-EGFP

Depositor

Lindy Barrett

Article

Hazelbaker et al Sci Rep. 2020 Jan 20;10(1):635. doi: 10.

Insert

TRE3G-dCas9-VPR-T2A-EGFP-CAG-TetON-NeoR-SA

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 6, 2020

Availability

Academic Institutions and Nonprofits only

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pT2ADW-lox-tdKeima-NES-lox-6011nls(ERK)-P2Av3-RGECO1

Plasmid

#162486

Purpose

For generating transgenic mice expressing ERK biosensor and RGECO1

Depositor

Michiyuki Matsuda

Article

Imanishi et al Am J Pathol. 2020 Oct 15. pii: S0002-944

Insert

ERK biosensor based on FRET, and RGECO1

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Dec. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pCX-NTOM-SpiCee

Plasmid

#140902

Purpose

mammalian expression of NTOM-SpiCee, a mitochondria-targeted variant of the calcium buffer SpiCee that prevents downstream signaling

Depositor

Xavier Nicol

Article

Ros et al Cell Rep. 2020 Jul 21;32(3):107934. doi:

Insert

NTOM-SpiCee

Use

Tags

mRFP

Expression

Mammalian

Mutation

Promoter

CAG

Available since

July 24, 2020

Availability

Academic Institutions and Nonprofits only

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AAV-GfaABC1D-ERCreER-4x6T

Plasmid

#196411

Purpose

Astrocytic expression of ERT2-Cre-ERT2 recombinase in AAV; astrocyte specificity enhanced with 4x6T miRNA targeting cassette

Depositor

Stanley Thomas Carmichael

Article

Gleichman et al Nat Commun. 2023 Nov 16;14(1):7426. doi:

Insert

ERT2-Cre-ERT2

Use

AAV; Astrocyte-selective

Tags

Expression

Mammalian

Mutation

Promoter

CAG and GfaABC1D

Available since

Feb. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pCIG2-Flag-mNuak1 WT

Plasmid

#168507

Purpose

For expression of Nuak1 (AMPK-related kinase also called ARK5/Omphk1)

Depositor

Franck Polleux

Article

Courchet et al Cell. 2013 Jun 20;153(7):1510-25. doi: 1

Insert

Nuak1 (Nuak1 Mouse)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

CAG

Available since

April 20, 2021

Availability

Academic Institutions and Nonprofits only

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pT077

Plasmid

#137879

Purpose

Donor vector for integration into the human AAVS1 safe harbor locus of Doxicycline-inducible KRAB-dCas9-IRES-EGFP

Depositor

Lindy Barrett

Article

Hazelbaker et al Sci Rep. 2020 Jan 20;10(1):635. doi: 10.

Insert

TRE3G-KRAB-dCas9-IRES-EGFP-CAG-TetON-NeoR-SA

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 6, 2020

Availability

Academic Institutions and Nonprofits only

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